Proceedings of the 10th International Colloquium on Paratuberculosis

#25 Isolation and identification <strong>of</strong> Mycobacterium avium subsp. paratuberculosis (Map) in
milk samples from a dairy herd in Brazil
Marcos José Pereira Gomes, Elise Yamasaki, Alexandre Galvão, Bernardo Stefano Bercht, Gustavo Geraldo
Medina Snel, José Artur Bogo Chies
Universidade Federal do Rio Grande do Sul - Porto Alegre, Brazil; Universidade Federal Rural do Rio de Janeiro -
Seropédica, Brazil
Mycobacterium avium subsp. paratuberculosis (Map) can be shed in <strong>the</strong> milk, feces or semen <strong>of</strong> infected animals.
Map infections are endemic in European and North American dairy cattle. Map infections are considered
rare and <strong>of</strong> minor importance in Brazil, despite that clinical cases have been recently reported and serological
estimates bear similarity to those obtained from o<strong>the</strong>r countries. The aim <strong>of</strong> <strong>the</strong> present study was to determine
if Map were present in samples <strong>of</strong> raw and pasteurized milk. Thirty-three samples <strong>of</strong> raw milk were collected
directly from <strong>the</strong> cooling tank at farms. The samples <strong>of</strong> raw milk were obtained from three dairy herds, two <strong>of</strong>
<strong>the</strong>m located in <strong>the</strong> state <strong>of</strong> Rio Grande do Sul and one located in <strong>the</strong> state <strong>of</strong> Rio de Janeiro, in which <strong>the</strong>
pathogen was isolated from a farm with recent cases <strong>of</strong> <strong>the</strong> disease and identified. A total <strong>of</strong> 379 samples <strong>of</strong>
milk sold in retail stores in Porto Alegre, sou<strong>the</strong>rn Brazil. Monthly samples <strong>of</strong> conventional, UHT and HTST
were treated and grown on HEYM for isolation <strong>of</strong> Map. Partial results show that Map could not be isolated from
<strong>the</strong> 379 samples <strong>of</strong> milk sold in 5 different retail stores. Of <strong>the</strong> 33 samples <strong>of</strong> raw milk samples, one was isolated
on HEYM. Based on cultural, phenotypic and molecular characteristics, <strong>the</strong> sample testing positive was
deemed to contain Map.
#39 Prevalence <strong>of</strong> Mycobacterium avium subsp. paratuberculosis in dairy cows by milk
nested PCR and its association with production and reproduction in Fars Province, Iran
Masoud Haghkhah, Maryam Ansari-Lari, Fereshteh Mahmoudi
Department <strong>of</strong> Pathobiology, School <strong>of</strong> Veterinary Medicine, Shiraz University, Iran; Department <strong>of</strong> Food Hygiene
and Public Health, School <strong>of</strong> Veterinary Medicine, Shiraz University, Iran
To evaluate <strong>the</strong> prevalence <strong>of</strong> Johne's disease and its impact on production and reproduction in dairy cows,
IS900 based nested PCR was used. Fur<strong>the</strong>rmore, data about production and reproduction were collected.
Overall, milk samples from 252 animals <strong>of</strong> 21 dairy herds were examined. In nested PCR, 8 samples (3.2%,
95%Cl: 1.35.1) were positive, corresponding to herd prevalence <strong>of</strong> 23.8% (95%Cl: 6.241.4). Statistical analysis
revealed no significant differences in genetic purity, total milk and lactation length between positive and negative
cows. However, 305 days milk (P=0.005) and calving interval (P=0.04) was significantly different between
herds with and without positive animals. Considering <strong>the</strong> effect <strong>of</strong> parity and genetic purity, 305 days milk was
8040 kg in negative herds, 520 kg higher than positive herds.
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