ON TESTIS AND EPlDlDYMlS OF RATS - Pondicherry University ...

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Table 31. Effect of TCDD on the production of superoxidc anion. nitric oxide and hydrogen peroxide in the caput, corpus and cauda epididyunides of adult rats TCDD and vitamin E Control Ing+20mg IOng+20mg 100ng+20mg Capur epididymh Superoxide anion' 1.69tt 0.09 1.76i 0.12 1.79k 0.18 1.76 + 0.17 Nitric oxideb 1.39i 0.19 1.43* 0.09 1.47i 0.16 1.49 i 0.11 Hydrogen peroxidec 13.43 i 1.40 11.75 i 1.07 11.73 * 1.39 11.47 2 0.73 Corpus epididymb Superoxide aniona 1.71 + 0.11 1.76+ 0.21 1.792 0.13 1.81 + 0.12 N~tric oxideb 1.372 0.13 1.37+ 0.09 139i 0.08 1.442 0.30 Hydrogen peroxidec 17.88 + 0.93 15.84 1.41 14.66 + 1.16 13.18 + 1.32 Coudo epidi+mis Superoxide anion' 1.82 + 0.09 1.92 * 0.13 1.87 1 0.36 1.84 i 0.14 Nitric oxideb 1.29f 0.21 1.37+ 0.09 1.33 1- 0.16 1.35+ 0.13 Hydrogen peroxidec 15.85 i 0.49 15.22 i 1.08 14.89 i 0.86 14.89 + 0.74 a nmole/ mini rng protein; mrnolel rnin/ mg protein nmol Hz02 generated1 minl mg protein The data are expressed as rneanYSD for six animals/ dose
Table 32. Effect of caadrninistration of TCDD and vitamin E on the levels of antioxidants in the caput, corpus and cauda epididymides of adult rats TCDD and vitamin E Control 1 ng+20mg 10ng+20mg 100ng+2Omg Glutathione' 1675399 15654.90 15826.31 160i4.11 a-~ocopherol~ 0.83i 0.1 1 0.84i 0.09 0.84t 0.40 0.84i 0.17 Corpus epididymis Glutathione' 149 f 6.14 152 i 2.62 150 i 3.21 151 i 4.26 a-Tocopherolb 0.84 i 0.16 0.844 2 0.21 0.84 2 0.20 0.82 i 0 I l Glutathione' 160 * 5.26 163 i 6.16 166 i 7.11 162 5 7.36 a-~oco~herol~ 0 85 f 0.1 l 0.86 i 0.26 0.85 + 0.21 0.85 + 0.32 nmoldmg protein; pg mg protein The data are expressed as rneanfSD for six animals/ dose
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FUNCTIONAL STUDIES ON THE EFFECTS O
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ACKNOWLEDGEMENTS I wish to express
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TABLE OF CONTENTS Page No ACKNOWLED
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TABLE OF CONTENTS (Continued) Deter
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TABLE OF CONTENTS (Continued) Effec
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TAULIC OF CON'I'ISN'I'S (C'untinued
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xi LIST OF TABLES (Continued) I'agc
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Table 24a Table 24b Table 24c Table
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Fig. 1 Fig. 2 Effect of TCDD on the
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Fig. 12c Fig. 12d Fig. 13 Fig. 14a
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LIST OF FIGURES (Continued) Fig. 23
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LH M PL mg mRNA N AD NADH NADP NADP
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layers, an outer layer of visceral
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tubular epithelium and continually
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lmn~unization of monkeys against FS
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and this reaction is catalyzed by t
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extratubular environment or synthes
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the most active in protein secretio
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1958). A rapid fall in serum testos
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have also been reported in the rats
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Effect of TCDD on heart muscle has
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1.4 Effect of oxidative stress on m
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2 SCOPE OF THE PRESENT STUDY There
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3 MATERIALS AND METHODS 3.1 Animals
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Subgroup III: Administration of TCD
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choppcd with the help of sharp razo
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anti-FSH antibody. After incubation
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lust sped (500 to 700 rpm) on an or
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3.13.4 Quantitative determinntiun u
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The plates were dried by inverting
Page 61 and 62: centrifuged at 3,500 g for I5 min o
Page 63 and 64: 3.17 Preparation of tiasue humogena
Page 65 and 66: supcrnutant was taken. Hlunk was pr
Page 67 and 68: 3.21.4 Assay of glutathione peroxid
Page 69: 4 RESULTS 4.1 Administration of TCD
Page 72 and 73: seminiferous tubules was found to b
Page 74 and 75: fractions of rat testis as compared
Page 76 and 77: 4.1.11.1 Effect of TCDD on the prod
Page 78 and 79: 4.2 Co-administration of TCDD and v
Page 80 and 81: 4.2.8 Effect of co-administration o
Page 82 and 83: 4.2.11.5 Effect of co-administratio
Page 84 and 85: Table I b. Effect of TCDD on the we
Page 86 and 87: Table 4. Effect of TCDD on the seru
Page 88 and 89: Table 7b. Effect of TCDD on the pro
Page 90 and 91: Table 8c. Effect of TCDD on the lev
Page 92 and 93: Table 9d. Effect of TCDD on the act
Page 94 and 95: Table 13. Effect of TCDD on the ant
Page 96 and 97: Table 15. Effect of TCDD on the lev
Page 98 and 99: Table 16c. Effcct of TCDD on the an
Page 100 and 101: Table 18a. Effect of co-administrat
Page 103 and 104: Table 21. Effect of co-administrati
Page 105 and 106: Table 24b. Effect of co-administrat
Page 107 and 108: Table 25c. Effect of co-administrat
Page 109 and 110: Table 26d. Effect of co-administrat
Page 111: Table 30. Effect of co-administrati
Page 115 and 116: Table 33c. Effcct of co-administrut
Page 117 and 118: 5 DISCUSSION 5.1 Significance of th
Page 119 and 120: Measurement of weights of the acces
Page 121 and 122: compartment of the cells (Chainy el
Page 123 and 124: the reduction in daily sperm produc
Page 125 and 126: eported in the TCDD-treated rats (M
Page 127 and 128: lilnction is to ililcrccpt lipid pc
Page 129 and 130: in the animals administered with TC
Page 131 and 132: protective effects on serum testost
Page 133: suygcstccl that vivamin 1: conld im
Page 136 and 137: Fig.4~. ENect of TCDD or TCDD and v
Page 138: tE W d In w it I I (,OCX) 3s PUB (,
Page 142 and 143: Fig. 7c. Effect of TCDD or TCDD and
Page 145 and 146: u 3 f ~ = m w * N O .- ~Slew aew en
Page 147 and 148: Fig.lOb. ENmt of TCDD or TCDD and v
Page 151: Fig. 1Ir Effect of TCDD or TCDD md
Page 158 and 159: Fig. 14c. Effect of TCDD or TCDD an
Page 160 and 161: Fig. 16b. Effect of TCDD or TCDD an
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Fig.18~. EKect of TCDD or TCDD and
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1 1.2 l4 Fig. 19b. ENect of TCDD or
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Fig. ZOb. Elkt of TCDD or TCDD and
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Fig. 21. Effect olTCDD or TCDD and
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Fig. 22c. Effect of TCDD or TCDD an
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Fig. 24b. Eflect ofTCDD or TCDD and
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7 REFERENCES Aitken RJ, Irvine DS,
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Chitm KC, Latchoumycandanc,C, Mathu
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Flsher JS, Turner KJ, Brown D, Shar
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Hornsby PJ. Regulation of cytochrom
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Latcboumycandane C, Chitra KC, Math
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Moore RW, Panona JA, Bookstaff RC,
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Rommerts FFG, Cooke BA, Van Der Kem
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Slaunwhite WR, Burgett MJ. h virro
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1.1 Preparation of dosing solution
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1.4.6 Wash concentrate One bottle,
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1.7.3 Estradiol controls Two vials.
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1.1 1.2 Orcinol(6%) 6 g of orcinol
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1.16.2 Horseradish peroxidase (8 Un
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1.20 Superoxide dismutase 1.20.1 Tr
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1.24.2 Thiobarbituric acid (TBA; 0.
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Chronic effect: of enclosulfan on t
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enbw1h1.W mu IUY Wcab unpiliml~t at
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cbniuis 11~11 b"nci:~lc ~wlive oxyg
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DNA, RNA ;uxl Wcin wcrr abarval, wh
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Mt~~m(10-lZsu&)vacob- Wfmn Ihehml ~
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New Delhi, llrliil Ib SCI~KK Rcm& M
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tiii~lc rcprmluction or rut (Cllitr
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wciglit (b.wt.) per day for 45 days
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0.1 00 01 " 0.8 I . .lo Dl* rcnn IW
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Chilr:~. K.C.. L!olnn!myci!~dum. C.
Page 228 and 229:
C. hteboumyeradlos . KC. CMtn . P.P
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I. 1 Ilal 01 2 1.7 # I c I ~ ~ L I
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Fin. L Corrclatwn bclwfcn "perm cou
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Iionshii nepmdvctivc mxicity ad upt
Page 240 and 241:
o. Glutathione reductage mg pmWn mg
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IOWAME -. Tor. #558517 PME: 9 SESS:
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JOBNAME: Rqm. Tm. M5W7 PAOB: 11 SBS
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t 02 ELSEVIER Toricology OM) (2W1)
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---- - wcre obtu~ncd from E-Monk. G
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3.2. A,III~AILNI syrlo~t of ,nIroch
Page 253 and 254:
Uc,#!dy. SC. N;tdcru. S , 1% Conlrb
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