ON TESTIS AND EPlDlDYMlS OF RATS - Pondicherry University ...

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JOBNAME: Rqm. Tm. M5W7 PAOB: 11 SBS: 5 OUmJT Tuc an 29 IO:W:~I zm2 MsbZla-m+m,iwasw-m cpididyrnis against lipid peraxidation mduced by me. thoxychlor by inCreasing antioxidant enzymes md de. crcains ROS geamtion. 1n addition, tosopbcml sup- PrcsEM lhe prcducdon of reactive oxidants and pmly impmvcl s pm motility (401. It has bsaa repartcd that vitamin E raises superoxide dismutasc activity in bovine sprm [411. Out results suggcst that exposure to melhoxychlor induces depletion in antioxidant defenrc system in the cpldidyrnie and opididymal sperm indisarlng methorychlor-induced oxidative smss in the epidrdymia. Thtr effect may lead to disruption in the functional integrity of cell orgmcller. We present study also suggests thal coadnunisvation of vitamin E with methorychlor prevents the advcne cffecfa of methorychlor on antioxidant status 8s well ar improving sperm motility in adult rats. In conflvrion the adverse effect of mcthorychior on male reproduction of rats may be due to the induction of oxidative rmrr. These effects could be nilevistcd by the antioxidant viumin E. IM authors aclmowledgc Dr. Ufe Tiem. Germany for lhe gifl of mcthoxychlor md the sfaff of the Bioinformat>ci Ccnlr. Pondichcny <strong>University</strong> for various foeilltier. C. L. thank8 lo thc Indian Council of Medical Rwrch. Ncw Delhi for Senior Research Fcilowrtup and P.P.M, acknawledger the Populntlon Council. New Yark for finunclul it*. r,stunce (Gnni Nu~.DY~.~U~P-Y~CMC and II1)P,lUYI
(=I 23. w- C Olln (LC W W. Ubrth d-1. Mrrmiaul.sWdmd-dmke-m w.7.1. v A = b T o l * o i . b p r r . 1261 W O ~ ~ ( a h r ~ d h m m s , - a d ~--I999.UhBdlt(Pr~U~. -.-,---. 1321 WLI + v- WN. sw*. oa guda* w gllimw - d ~ ~ p l o i ( d n I l . b Q i n Med 1967:701SB-69. P31 Fid; E K dPi Y. S-M boa ad MIL -a br . . Anr LU. 19z-ml 1351 MSh. PP. Oatop.dgq. 5.190 d- .rr r- - rn n-ldrnd m- (n rr ICL 8- ,.- 873-G ...-" 1361 om. 5 w. I, coos. RL. r 4 lops m -k ad ~--%w..*alm~llrt mdbchmarhhol*-phdt-y.)I.aLnldpDarttD in rmb DI TmM. M H..h4 IS, 37-47 1371 mloar.r-.r.-~-d-ccn- M.-.D.-J. 19W.01.18*1- d~M-mdElPUCIDni.dmbY-.nda-Wd
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FUNCTIONAL STUDIES ON THE EFFECTS O
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ACKNOWLEDGEMENTS I wish to express
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TABLE OF CONTENTS Page No ACKNOWLED
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TABLE OF CONTENTS (Continued) Deter
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TABLE OF CONTENTS (Continued) Effec
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TAULIC OF CON'I'ISN'I'S (C'untinued
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xi LIST OF TABLES (Continued) I'agc
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Table 24a Table 24b Table 24c Table
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Fig. 1 Fig. 2 Effect of TCDD on the
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Fig. 12c Fig. 12d Fig. 13 Fig. 14a
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LIST OF FIGURES (Continued) Fig. 23
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LH M PL mg mRNA N AD NADH NADP NADP
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layers, an outer layer of visceral
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tubular epithelium and continually
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lmn~unization of monkeys against FS
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and this reaction is catalyzed by t
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extratubular environment or synthes
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the most active in protein secretio
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1958). A rapid fall in serum testos
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have also been reported in the rats
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Effect of TCDD on heart muscle has
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1.4 Effect of oxidative stress on m
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2 SCOPE OF THE PRESENT STUDY There
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3 MATERIALS AND METHODS 3.1 Animals
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Subgroup III: Administration of TCD
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choppcd with the help of sharp razo
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anti-FSH antibody. After incubation
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lust sped (500 to 700 rpm) on an or
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3.13.4 Quantitative determinntiun u
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The plates were dried by inverting
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centrifuged at 3,500 g for I5 min o
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3.17 Preparation of tiasue humogena
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supcrnutant was taken. Hlunk was pr
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3.21.4 Assay of glutathione peroxid
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4 RESULTS 4.1 Administration of TCD
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seminiferous tubules was found to b
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fractions of rat testis as compared
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4.1.11.1 Effect of TCDD on the prod
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4.2 Co-administration of TCDD and v
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4.2.8 Effect of co-administration o
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4.2.11.5 Effect of co-administratio
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Table I b. Effect of TCDD on the we
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Table 4. Effect of TCDD on the seru
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Table 7b. Effect of TCDD on the pro
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Table 8c. Effect of TCDD on the lev
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Table 9d. Effect of TCDD on the act
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Table 13. Effect of TCDD on the ant
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Table 15. Effect of TCDD on the lev
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Table 16c. Effcct of TCDD on the an
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Table 18a. Effect of co-administrat
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Table 21. Effect of co-administrati
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Table 24b. Effect of co-administrat
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Table 25c. Effect of co-administrat
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Table 26d. Effect of co-administrat
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Table 30. Effect of co-administrati
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Table 32. Effect of caadrninistrati
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Table 33c. Effcct of co-administrut
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5 DISCUSSION 5.1 Significance of th
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Measurement of weights of the acces
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compartment of the cells (Chainy el
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the reduction in daily sperm produc
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eported in the TCDD-treated rats (M
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lilnction is to ililcrccpt lipid pc
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in the animals administered with TC
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protective effects on serum testost
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suygcstccl that vivamin 1: conld im
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Fig.4~. ENect of TCDD or TCDD and v
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tE W d In w it I I (,OCX) 3s PUB (,
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Fig. 7c. Effect of TCDD or TCDD and
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u 3 f ~ = m w * N O .- ~Slew aew en
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Fig.lOb. ENmt of TCDD or TCDD and v
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Fig. 1Ir Effect of TCDD or TCDD md
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Fig. 14c. Effect of TCDD or TCDD an
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Fig. 16b. Effect of TCDD or TCDD an
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Fig.18~. EKect of TCDD or TCDD and
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1 1.2 l4 Fig. 19b. ENect of TCDD or
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Fig. ZOb. Elkt of TCDD or TCDD and
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Fig. 21. Effect olTCDD or TCDD and
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Fig. 22c. Effect of TCDD or TCDD an
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Fig. 24b. Eflect ofTCDD or TCDD and
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7 REFERENCES Aitken RJ, Irvine DS,
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Chitm KC, Latchoumycandanc,C, Mathu
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Flsher JS, Turner KJ, Brown D, Shar
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Hornsby PJ. Regulation of cytochrom
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Latcboumycandane C, Chitra KC, Math
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Moore RW, Panona JA, Bookstaff RC,
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Rommerts FFG, Cooke BA, Van Der Kem
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Slaunwhite WR, Burgett MJ. h virro
Page 193 and 194: 1.1 Preparation of dosing solution
Page 195 and 196: 1.4.6 Wash concentrate One bottle,
Page 197 and 198: 1.7.3 Estradiol controls Two vials.
Page 199 and 200: 1.1 1.2 Orcinol(6%) 6 g of orcinol
Page 201 and 202: 1.16.2 Horseradish peroxidase (8 Un
Page 203 and 204: 1.20 Superoxide dismutase 1.20.1 Tr
Page 205 and 206: 1.24.2 Thiobarbituric acid (TBA; 0.
Page 207 and 208: Chronic effect: of enclosulfan on t
Page 209: enbw1h1.W mu IUY Wcab unpiliml~t at
Page 212 and 213: cbniuis 11~11 b"nci:~lc ~wlive oxyg
Page 214 and 215: DNA, RNA ;uxl Wcin wcrr abarval, wh
Page 216 and 217: Mt~~m(10-lZsu&)vacob- Wfmn Ihehml ~
Page 218 and 219: New Delhi, llrliil Ib SCI~KK Rcm& M
Page 220 and 221: tiii~lc rcprmluction or rut (Cllitr
Page 222 and 223: wciglit (b.wt.) per day for 45 days
Page 224 and 225: 0.1 00 01 " 0.8 I . .lo Dl* rcnn IW
Page 226 and 227: Chilr:~. K.C.. L!olnn!myci!~dum. C.
Page 228 and 229: C. hteboumyeradlos . KC. CMtn . P.P
Page 230 and 231: I. 1 Ilal 01 2 1.7 # I c I ~ ~ L I
Page 232 and 233: Fin. L Corrclatwn bclwfcn "perm cou
Page 235: Iionshii nepmdvctivc mxicity ad upt
Page 240 and 241: o. Glutathione reductage mg pmWn mg
Page 242 and 243: IOWAME -. Tor. #558517 PME: 9 SESS:
Page 246 and 247: t 02 ELSEVIER Toricology OM) (2W1)
Page 248 and 249: ---- - wcre obtu~ncd from E-Monk. G
Page 250: 3.2. A,III~AILNI syrlo~t of ,nIroch
Page 253 and 254: Uc,#!dy. SC. N;tdcru. S , 1% Conlrb
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