ON TESTIS AND EPlDlDYMlS OF RATS - Pondicherry University ...
ON TESTIS AND EPlDlDYMlS OF RATS - Pondicherry University ...
ON TESTIS AND EPlDlDYMlS OF RATS - Pondicherry University ...
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'lstrr,tb~xr 171 i?lYJ?I 127 /.IS Ill<br />
rcuctiw oxygen rwim in liver (Sugihilrs ct ill.,<br />
2OUI). In the pracnl aludy adminiutrution ol'<br />
TCDI) dccwd tla ttctivitim of ci~t~ilitw, xc~pr.<br />
oxide diuilutuw, &IuIathio~ic rcducttt~, itlid 8111-<br />
tutliiolw proxiduw. wl~ile the lcvcls of bydrop11<br />
peroxide and lipid peroxidntion incrwscd in tlrc<br />
mitochondrial and microrom~l fnctlonr of the<br />
testis. Mitochondrial rapintion is the main biological<br />
wurm of tuproxide anion radicals undcr<br />
physiological conditions. The mtioxidanl enzyme<br />
supemaide dimutasc hps bmn rhown to aculerute<br />
the dimutation of the superoxide anion into<br />
hydrogen peroxide (Nirscn and Knyscl. 1983).<br />
Cntulase allows degmdation of hydrogen peroxide<br />
into water und oxygcn (Jeulin el al., 1989) and llic<br />
glutathione pcroxidue/reductue system ha^ been<br />
rhown to calalysc the degradation of hydrogen<br />
peroxide and lipid hydroperoxide by using re.<br />
dud glutathione (Alvam and Storey. 1989).<br />
The reduction in the activitin of antioxidant enzymes<br />
and incruuc in hydrogen peroxide and<br />
lipid peroxidation could reflect the adverse effect<br />
of TCDD on tho untioxidunt system in testis<br />
subcellular frnctions. Oxidative stress has been<br />
shown to occur primarily in the mitochondria<br />
fol\owed by microsomn. Orievau and Lunnou<br />
11097) rcprtnl that ROS such as HIOt uppca,rs<br />
to k u key agent in the cytotoxic effects In<br />
spemlowe and in udJition to their dim effect<br />
on cellular constituent produce oxidative stress by<br />
dwrc~~sil~y the c11zymatic JeRm of the testis.<br />
Un& high ROS mnrrntrntions pre-dumuged<br />
rpcnnutowu an exposed to lipid peroxidatioli by<br />
polyunwturuted fatty acids (Ichikawrr ct al..<br />
1999). The mitochondriul and microsomal membrann<br />
have ken reported to undergo permurbility<br />
changes following enhanad Lipid peroxidation<br />
and Jutathione depletion (Chancc ct al.. 1979).<br />
One of the important upatl of anlioxidwt en.<br />
rymer ii their nature of synergistic functioning: a<br />
decnrsc in superoxide dirmulav clctivity hm been<br />
shown to increase the level of supcroride anion,<br />
which is known to inactivate catlrlrro llctivily<br />
(KOIO and Fridovich. 1982). Similarly. when<br />
wtahuc or gluuthbne psmxiduc faib to climinate<br />
H,O1 from the all, the accumulated H,O,<br />
hru been ahown to caw inactivation of suproxide<br />
dismuw (Sine1 md Garber. 1981). Reactive<br />
oxygcli spccics Iisr bceci sliown 111 d;~tiii~yc ;llliioal<br />
ill1 ~llscromolcculcr. viz. prolein, lipids. ccc.. i111d<br />
lllc cliiinpr in llic rcprtnh~ctivc orpiill wciphts<br />
111:ly slso Iw duc lo iIi~~l~ii$c ~IIIIIIIC~~I hy IUIICI~VC<br />
Uxygc11 rwiw (&l~~ultdt%rl: IDYO). 'I'llc ~.acllla<br />
sugycst thut the low doses or TCDV clicil dcplc.<br />
lion in antioxidunt defense system diflerentislly in<br />
mitmhondrial and microsonial rrilctions indici~ling<br />
TCDD-induced oxidative strcsr ill tculis suhccllular<br />
fractions. This may lend to disruption of<br />
functional integrity of cell organelles. In conclusion<br />
the adverse long. term effects or low doscs or<br />
TCDD on male reproduction of mts may be due<br />
lo the induction of oxidative stress in testis.<br />
Acknowledgments<br />
Tlie aulhors ock~~owledge Dr Slcplien Safc. TX.<br />
USA, for the generous girt of TCDD and thc still'l'<br />
or the Bioinrormutia Center. <strong>Pondicherry</strong> Unlversity.<br />
for various incilitics. C.L. acknowledges<br />
the Indian Council of Medicnl Rcseilrch. New<br />
Delhi. for Senior Research Fellowship. K.C. ilck~iowledges<br />
the Lady Tstu Meniorial Trust.<br />
Munibai for u Junior Scholursliip ~IIIJ P.I1.M.<br />
ucknowledges Ihe Population Council. New Yolk<br />
for financial assistance (Grant Nos. B99.047P-91<br />
ICMC md B99.048RIlCMC).<br />
Ra. 23. 77-90.<br />
Bury. I.A.. Ausl. S.D.. 1976. Lrclo~roridlw cill;tlyml Ilpal<br />
proriblion of micrororn~l and ~~rlilicrrl n!nn~brnnn.<br />
Biochim. Biophy+ Acts 444. 192-201.<br />
Curlbrre. I., Mmnmik. 8.. 1975. Purifimlion und chlmtctcrl.<br />
ulion d Ur Ilrvanzym plulrlhiom rrJurlax from nil<br />
livcr. J. Bbl. M. 250. Yl4-5480.<br />
Chainy, O.B.N.. S.nunwrry. 6.. Salnanla. L. 1991. Tnlov<br />
temne-induced sbnp in taticulur nnlioxiJilnl ~~(ICIII<br />
Androlopiu 29, 341-MY.<br />
Chuncc. 0.. Sie* H.. Bonrit. A,. 1979, llydrapcro~de<br />
nlslubolirm in murnmulwn orylnl. PII).riol, Kcv 59. $27-<br />
514.<br />
Chw. S.E.. 2WO. Endwrinr disruploi. innd nlalr rrpruducltsc<br />
futction - a &an ncuka. tat. 1. Andrul. 21. 45-46.