ON TESTIS AND EPlDlDYMlS OF RATS - Pondicherry University ...

1.6.2 Testosterone standards
One vial, 0.5 mL, labeled A contained 0 ng/ mL testosterone and six vials, 0.5
mL each, labeled B-G, contained concentrations of approximately 0.1. 0.5.
2.5, 5.0, 10 and 25 ngl mL testosterone in human serum with a non-mercury
preservative.
1.6.3 Testosterone controls
Two viats, 0.5 mL each, Levels I and II, contained low and high
concentrations of testosterone in human serum with a non-mercury
preservative.
1.6.4 Testosterone-Enzyme Conjugate concentrate
One vial, 0.3 mL, contained testosterone conjugated to horseradish peroxidase
in a protein-based (BSA) buffer with a non-mercury preservative. The
Antibody-Enzyme Conjugate concentrate was diluted at a ratio of 1 part into
50 parts of the assay buffer.
1.6.5 Testosterone antiserum
One vial, I I mL, contained rabbit anti-testosterone (polyclonal) serum in
protein based (BSA) buffer with a non-mercury preservative.
1.6.6 Tetramethylbenzidine chromogen solution (TMB)
One amber bottle. I I mL contained a solution of TMB in citrate buffer with
hydrogen peroxide.
1.6.7 Wash concentrate
One bottle, 60 mL contained buffered saline with a nonionic detergent. Diluted
25-fold with deionized water prior to use.
1.7 Serum estmdiol
1.7.1 Anti-Esuadiol-Coated microtitration strips
One strip holder contained 96 polystyrene microtitre wells coated with rabbit
anti-estradiol IgG immobilized to the inside wall of each well. Stored at 2-8°C
in the resalable pouch with a desiccant to protect from moisture.
1.7.2 Esuadiol standards
One vial, 3 mL, labeled A contained 0 pgl mL and six vials, 1 rnL each,
labeled B-G, contained concentrations of approximately 20, 50, 250, 750.
2000 and 6000 pgl mL estradiol in human serum with a non-mercury
preservative.