ON TESTIS AND EPlDlDYMlS OF RATS - Pondicherry University ...

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Fin. L Corrclatwn bclwfcn "perm counts and DNA cootent$ or Fil. 7. Urea of TCDD on lied mlidrtion in the s~ididvrnal nl cpididymli rprm. A paaltivc corrclaten (r-0.9% n-24) war Grin or la.. 7hc m m adktyu in m~rdm.i" found between rprm wunl and DNA contcnl pr mtnulc and rnillignnn or mein Ot millisnm 01 DNA. n o data urn n W M munlSD Iw ax uprimcnUl and am control mum. PcO.05 lm~erldx) il lo indiate a rienificant activity (Kono and Fridovich 1982). Cataiasc or glutathione peroxidae rails to eliminate HZ02 from the cell, and the accumulated H202 has been shown to cause inactivation of superoxidc dismutase (Sine1 and Garber 1981). Griveau and Lannou (1997) reported lhal ROS dovl of TCDD (0.15 ndkg per day on 5 days/week for 13 weeks) wuld induce oxidative stmu in liver, spleen, lung and kidncy following rubchronic exposure in mice (Skwk ct ill. WOO). In tho orrscat rtudv administration of TCDD cuuml II ducll& In the sperin wunts, which 1s wnrrclcnl wtth esrlter reporu (Fwt el al 1991. Gra) cl uI 1997) S~nce a wry ugn~hcrat wrnlauon (r- 0 95. 18-24) was ohncrvfd bitwan cprm counb and DNA conlcnb in cpididyntnl,sperm. DNA wnlcnt wus routinely ucsd M an indicator of sprm wunt (Mukherja cl ul. 19921. Adminiatnlion of TCDD decreased the hydrogen peroxide and lipid peroddauon roo in the enididvmal soerm of mla in tams of boLb ~mlcin and ~ N *LI~~I; A doso-response wsr not obscr;od wtl! re. galrd lo .any ofllr hit)~.hw~luI ptmmctcn In our rlvdx\. which is auito similar to (he mulb obrwed in studies of the ctTcct.of TCDD on the hepatic and braln tluues of nu ( Hmun el d. 2001). M~tochondnPl mplntlon tr the main biolo+icnl mum of tuamxidc anion radials in physiolopi&l conditions. 7% antioxidant snrymc superoxide dimuusc lsaknla the diimuulion of Ihc runeroxidc anion into hvdroncn mxidc. Cnlal;~se ilc- such as HIOl appear to be key agents in the cytoloxic elTects in spennalozoa, and in addition lo their direct erect on the cellular wnstimcnt, to produce oxida~ve stress by darcasing the enzymatic defenses. Thc rise in the kvels of hydrogen peroxide generation by 160-170% reflccls the induction bv TCDD of the oroduction of mctlve oxygen rpcctcs~lhat arc no1 e.ttn'~nated b\ lhr ant~oxtdanc enzyme, rush as catalase and g.u~ath~one reductase/proxidase systems, and this can induce lipid oeroxidation. The wssibilitv of eflects other than the uppnrcnt darease In unt!oa~d.lnt actl\lltcs tn caus ng 1hc tncw m peroude product.on also cdnnol bs r~lcd our. Cytoplasm of the spermatozoa is extremely limited in volume and localization. so that the ~olvunsaturated fatty aads lhsl nhound In Ihe eprm plkm'a mcmnrd.w urc vcry suscept~blc to ROS ultdck (Al.kcn et ul tYSJ, When ROS &nczntrationr are high, predamaged spcrmatozoa are exposed to lipid peroxidation by polyunsatunlcd fatty acids (Ichikawa el al. 1999). The sperm membranes have been teported to undergo permeabilily chanua followinn enhanced linid mroxidation and glut~htone deplc
IIR h r k a ~ r Ilw ~ ;njllmr~ l ~ Ilnionk Dr. Stcrhcn Sitrc. TEXIIX. USA, li,r Ihr: gctwmu\ gn uITCUU, and Ihc rlail of thc 01otnr0rm:ltin Ccnlcr. Pondichcrry Uncvcnily lot ~riow Iscilain. C L tr the Iholder oli, Setunr Rwurch Fcli~w~hip Im thc Indian ~ ...., . ", ('rrhg iron? tk LnJy Tala Memorial Tnul. Mumhi; w t r and bn~n ((PYOI d nu Inn eubshronic 1; ilnd P.P M hi83 refcivd Ilwndal ;,nmtlnn: rmm tk Popvialion m~nlumo~TCDD.ndlU n IApplTrmcol2l~2lI-219 Tclt~ml. Nrv Yolk r$mnla nn. RW YlP.P/ICMC.nd BPP.MRRI lehok.m T . ~ o d l ~ . ~ h mWB(1999) o n ~ R- oxyen Il'M('1 'Ihc ia~*~llvn LILYI:IP tlnill IIK cx~ri~ncnln done Jurin~ won tnfluaa Ik o a m a raa.on hut nn r.ain anans. tllru. ato~lnz R)III~~Y wvlh llle cl4rrelll 181~1 or lhr co~ntry. J&,G&:fi
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FUNCTIONAL STUDIES ON THE EFFECTS O
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ACKNOWLEDGEMENTS I wish to express
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TABLE OF CONTENTS Page No ACKNOWLED
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TABLE OF CONTENTS (Continued) Deter
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TABLE OF CONTENTS (Continued) Effec
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TAULIC OF CON'I'ISN'I'S (C'untinued
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xi LIST OF TABLES (Continued) I'agc
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Table 24a Table 24b Table 24c Table
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Fig. 1 Fig. 2 Effect of TCDD on the
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Fig. 12c Fig. 12d Fig. 13 Fig. 14a
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LIST OF FIGURES (Continued) Fig. 23
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LH M PL mg mRNA N AD NADH NADP NADP
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layers, an outer layer of visceral
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tubular epithelium and continually
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lmn~unization of monkeys against FS
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and this reaction is catalyzed by t
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extratubular environment or synthes
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the most active in protein secretio
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1958). A rapid fall in serum testos
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have also been reported in the rats
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Effect of TCDD on heart muscle has
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1.4 Effect of oxidative stress on m
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2 SCOPE OF THE PRESENT STUDY There
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3 MATERIALS AND METHODS 3.1 Animals
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Subgroup III: Administration of TCD
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choppcd with the help of sharp razo
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anti-FSH antibody. After incubation
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lust sped (500 to 700 rpm) on an or
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3.13.4 Quantitative determinntiun u
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The plates were dried by inverting
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centrifuged at 3,500 g for I5 min o
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3.17 Preparation of tiasue humogena
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supcrnutant was taken. Hlunk was pr
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3.21.4 Assay of glutathione peroxid
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4 RESULTS 4.1 Administration of TCD
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seminiferous tubules was found to b
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fractions of rat testis as compared
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4.1.11.1 Effect of TCDD on the prod
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4.2 Co-administration of TCDD and v
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4.2.8 Effect of co-administration o
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4.2.11.5 Effect of co-administratio
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Table I b. Effect of TCDD on the we
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Table 4. Effect of TCDD on the seru
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Table 7b. Effect of TCDD on the pro
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Table 8c. Effect of TCDD on the lev
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Table 9d. Effect of TCDD on the act
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Table 13. Effect of TCDD on the ant
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Table 15. Effect of TCDD on the lev
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Table 16c. Effcct of TCDD on the an
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Table 18a. Effect of co-administrat
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Table 21. Effect of co-administrati
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Table 24b. Effect of co-administrat
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Table 25c. Effect of co-administrat
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Table 26d. Effect of co-administrat
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Table 30. Effect of co-administrati
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Table 32. Effect of caadrninistrati
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Table 33c. Effcct of co-administrut
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5 DISCUSSION 5.1 Significance of th
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Measurement of weights of the acces
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compartment of the cells (Chainy el
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the reduction in daily sperm produc
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eported in the TCDD-treated rats (M
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lilnction is to ililcrccpt lipid pc
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in the animals administered with TC
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protective effects on serum testost
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suygcstccl that vivamin 1: conld im
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Fig.4~. ENect of TCDD or TCDD and v
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tE W d In w it I I (,OCX) 3s PUB (,
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Fig. 7c. Effect of TCDD or TCDD and
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u 3 f ~ = m w * N O .- ~Slew aew en
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Fig.lOb. ENmt of TCDD or TCDD and v
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Fig. 1Ir Effect of TCDD or TCDD md
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Fig. 14c. Effect of TCDD or TCDD an
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Fig. 16b. Effect of TCDD or TCDD an
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Fig.18~. EKect of TCDD or TCDD and
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1 1.2 l4 Fig. 19b. ENect of TCDD or
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Fig. ZOb. Elkt of TCDD or TCDD and
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Fig. 21. Effect olTCDD or TCDD and
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Fig. 22c. Effect of TCDD or TCDD an
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Fig. 24b. Eflect ofTCDD or TCDD and
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7 REFERENCES Aitken RJ, Irvine DS,
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Chitm KC, Latchoumycandanc,C, Mathu
Page 181 and 182: Flsher JS, Turner KJ, Brown D, Shar
Page 183 and 184: Hornsby PJ. Regulation of cytochrom
Page 185 and 186: Latcboumycandane C, Chitra KC, Math
Page 187 and 188: Moore RW, Panona JA, Bookstaff RC,
Page 189 and 190: Rommerts FFG, Cooke BA, Van Der Kem
Page 191 and 192: Slaunwhite WR, Burgett MJ. h virro
Page 193 and 194: 1.1 Preparation of dosing solution
Page 195 and 196: 1.4.6 Wash concentrate One bottle,
Page 197 and 198: 1.7.3 Estradiol controls Two vials.
Page 199 and 200: 1.1 1.2 Orcinol(6%) 6 g of orcinol
Page 201 and 202: 1.16.2 Horseradish peroxidase (8 Un
Page 203 and 204: 1.20 Superoxide dismutase 1.20.1 Tr
Page 205 and 206: 1.24.2 Thiobarbituric acid (TBA; 0.
Page 207 and 208: Chronic effect: of enclosulfan on t
Page 209: enbw1h1.W mu IUY Wcab unpiliml~t at
Page 212 and 213: cbniuis 11~11 b"nci:~lc ~wlive oxyg
Page 214 and 215: DNA, RNA ;uxl Wcin wcrr abarval, wh
Page 216 and 217: Mt~~m(10-lZsu&)vacob- Wfmn Ihehml ~
Page 218 and 219: New Delhi, llrliil Ib SCI~KK Rcm& M
Page 220 and 221: tiii~lc rcprmluction or rut (Cllitr
Page 222 and 223: wciglit (b.wt.) per day for 45 days
Page 224 and 225: 0.1 00 01 " 0.8 I . .lo Dl* rcnn IW
Page 226 and 227: Chilr:~. K.C.. L!olnn!myci!~dum. C.
Page 228 and 229: C. hteboumyeradlos . KC. CMtn . P.P
Page 230 and 231: I. 1 Ilal 01 2 1.7 # I c I ~ ~ L I
Page 235: Iionshii nepmdvctivc mxicity ad upt
Page 240 and 241: o. Glutathione reductage mg pmWn mg
Page 242 and 243: IOWAME -. Tor. #558517 PME: 9 SESS:
Page 244 and 245: JOBNAME: Rqm. Tm. M5W7 PAOB: 11 SBS
Page 246 and 247: t 02 ELSEVIER Toricology OM) (2W1)
Page 248 and 249: ---- - wcre obtu~ncd from E-Monk. G
Page 250: 3.2. A,III~AILNI syrlo~t of ,nIroch
Page 253 and 254: Uc,#!dy. SC. N;tdcru. S , 1% Conlrb
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