ON TESTIS AND EPlDlDYMlS OF RATS - Pondicherry University ...
ON TESTIS AND EPlDlDYMlS OF RATS - Pondicherry University ...
ON TESTIS AND EPlDlDYMlS OF RATS - Pondicherry University ...
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3 MATERIALS <strong>AND</strong> METHODS<br />
3.1 Animals<br />
Male rats of Wistar strain obtained from the Central Animal House, Jawaharlal<br />
Institute of Postgraduate Medical Education and Research (JIPMER), <strong>Pondicherry</strong><br />
were used in the study.<br />
3.2 Maintenance<br />
Animals were housed in polypropylene cages ( l8x 10"x 8") lined with paddy<br />
husk. under a well regulated light and dark (l2h: 12h) scheduled. Rats were fed with<br />
pelleted food (Kamedhenu Agencies. Bangalore, India) and water ad libitum. Growth<br />
of'the animals was monitored regularly and animals displaying poor growth rate were<br />
discarded from the experiments.<br />
3.3 Chemicals<br />
2.3.7.8-tetrachlorodibenzo-p-dioxin (TCDD) was a gift from Dr. Stefen Safe,<br />
Department of Veterinary Anatomy and Public Health. A & M <strong>University</strong>, Texas.<br />
USA. Trichloroacetic acid, dehydrocpiandrosterone and androsterone 3, 17-dione<br />
were purchased from Sigma Chemical Company, MO, USA. Thiobarbituric acid and<br />
malondialdehyde were obtained from E-Merck, Germany.<br />
For hormone assays.<br />
ELSA kits were obtained from Diagnostic Systems Laboratories, Inc. Webster,<br />
Texas, USA. Ham's F12 medium, vitamin E (DL-tocopherol, 99%), horseradish<br />
peroxidase (HRP), niwtinamide adenine dinucleotide (NAD), nicotinamide adenine<br />
dinucleotide reduced (NADH), nicotinamide adenine dinucleotide phosphate<br />
WADP), bovine =rum albumin (BSA), deoxyribonucleic acid (DNA). ribonucleic<br />
acid (RNA) and 2,6dichlorophenol indophenol (DCPIP) were obtained from Himedia<br />
Laboratory Pvt. Ltd., Mumbai, India. Nicotinamide adenine dinucleotide phosphate<br />
reduced (NADPH) and N-(I-naphthyl) ethylene diamine di-hydrochloride were