ON TESTIS AND EPlDlDYMlS OF RATS - Pondicherry University ...

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DECLARATION I hereby declare that the work presented in this thesis has been carried out by me under the supervision of Dr. P.P. Mathur, Professor, School of Life Sciences, Pondicherry University. To the best of my knowledge, no part of this thesis has been submitted for the award of a research degree of any other University. J c. i. Lwd-+'- (C. l,A~~CliO~lMY('ANl)ANl~) Candidate
ACKNOWLEDGEMENTS I wish to express my sincere gratitude to Dr. P. P. Mathur, Professor, School of Life Sciences, Pondicheny University, Pondicherry for his invaluable guidance, unceasing help, unerring supervision, critical suggestions, encouragement and the freedom with which I could work in his laboratory throughout the course of my research work. I express my sincere gratitude to Dr. E. Vijayan, Professor and Head, School of Life Sciences, Pondicherry University, Pondicheny for providing me laboratory facilities. My thanks are due to the other faculty members of my department Dr. B. Ka~abiran, Dr. A. Ramachandra Reddy and Dr. K. Srikumar, School of Life Sciences, Pondicherry University for their encouragement. My sincere gratitude to Dr. Stefen Safe, Department of Veterinary Anatomy and Public Health, A & M University, Texas, USA for the gift of TCDD without which I could not complete my work successfully. My sincere thanks to Dr. Dheer Singh, Scientist, National Dairy Research Institute, Karnal and Dr. R.R. Manimaran, Department of Pharmacology and Therapeutics. McGill University, Montreal. Canada, for their valuahlc suggcslions and encouragements. 1 would also thank Dr. S.C. Parija, Officer in-Charge and other staff of the Central Animal House. JIPMER, Pondicherry for providing animals. I am highly thankful to Dr. P.P. Mathur, Officer in-Charge and Mr. M. Sundaramohan and Ms. V. Amoudha Staff of Bioinformatics Centre, Distributed Information Sub-centre, Pondicherry University, Pondicheny for providing me the necessary facilities for literature search and other help. I sincerely thanks to Ms. K.C. Chitra, Research Scholar, School of Life Sciences, Pondicheny University for her continuous help throughout my experiments. I also thank Ms. P. Poonkothai, Ms. R. Sujatha, Mr. M. Arul Murugan, Ms. Bindhu
Page 1: FUNCTIONAL STUDIES ON THE EFFECTS O
Page 5 and 6: TABLE OF CONTENTS Page No ACKNOWLED
Page 7 and 8: TABLE OF CONTENTS (Continued) Deter
Page 9 and 10: TABLE OF CONTENTS (Continued) Effec
Page 11 and 12: TAULIC OF CON'I'ISN'I'S (C'untinued
Page 13 and 14: xi LIST OF TABLES (Continued) I'agc
Page 15 and 16: Table 24a Table 24b Table 24c Table
Page 17 and 18: Fig. 1 Fig. 2 Effect of TCDD on the
Page 19 and 20: Fig. 12c Fig. 12d Fig. 13 Fig. 14a
Page 21 and 22: LIST OF FIGURES (Continued) Fig. 23
Page 23 and 24: LH M PL mg mRNA N AD NADH NADP NADP
Page 25 and 26: layers, an outer layer of visceral
Page 27 and 28: tubular epithelium and continually
Page 29 and 30: lmn~unization of monkeys against FS
Page 31 and 32: and this reaction is catalyzed by t
Page 33 and 34: extratubular environment or synthes
Page 35 and 36: the most active in protein secretio
Page 37 and 38: 1958). A rapid fall in serum testos
Page 39 and 40: have also been reported in the rats
Page 41 and 42: Effect of TCDD on heart muscle has
Page 43 and 44: 1.4 Effect of oxidative stress on m
Page 45 and 46: 2 SCOPE OF THE PRESENT STUDY There
Page 47 and 48: 3 MATERIALS AND METHODS 3.1 Animals
Page 49 and 50: Subgroup III: Administration of TCD
Page 51 and 52: choppcd with the help of sharp razo
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anti-FSH antibody. After incubation
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lust sped (500 to 700 rpm) on an or
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3.13.4 Quantitative determinntiun u
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The plates were dried by inverting
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centrifuged at 3,500 g for I5 min o
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3.17 Preparation of tiasue humogena
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supcrnutant was taken. Hlunk was pr
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3.21.4 Assay of glutathione peroxid
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4 RESULTS 4.1 Administration of TCD
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seminiferous tubules was found to b
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fractions of rat testis as compared
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4.1.11.1 Effect of TCDD on the prod
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4.2 Co-administration of TCDD and v
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4.2.8 Effect of co-administration o
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4.2.11.5 Effect of co-administratio
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Table I b. Effect of TCDD on the we
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Table 4. Effect of TCDD on the seru
Page 88 and 89:
Table 7b. Effect of TCDD on the pro
Page 90 and 91:
Table 8c. Effect of TCDD on the lev
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Table 9d. Effect of TCDD on the act
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Table 13. Effect of TCDD on the ant
Page 96 and 97:
Table 15. Effect of TCDD on the lev
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Table 16c. Effcct of TCDD on the an
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Table 18a. Effect of co-administrat
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Table 21. Effect of co-administrati
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Table 24b. Effect of co-administrat
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Table 25c. Effect of co-administrat
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Table 26d. Effect of co-administrat
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Table 30. Effect of co-administrati
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Table 32. Effect of caadrninistrati
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Table 33c. Effcct of co-administrut
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5 DISCUSSION 5.1 Significance of th
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Measurement of weights of the acces
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compartment of the cells (Chainy el
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the reduction in daily sperm produc
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eported in the TCDD-treated rats (M
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lilnction is to ililcrccpt lipid pc
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in the animals administered with TC
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protective effects on serum testost
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suygcstccl that vivamin 1: conld im
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Fig.4~. ENect of TCDD or TCDD and v
Page 138:
tE W d In w it I I (,OCX) 3s PUB (,
Page 142 and 143:
Fig. 7c. Effect of TCDD or TCDD and
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u 3 f ~ = m w * N O .- ~Slew aew en
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Fig.lOb. ENmt of TCDD or TCDD and v
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Fig. 1Ir Effect of TCDD or TCDD md
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Fig. 14c. Effect of TCDD or TCDD an
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Fig. 16b. Effect of TCDD or TCDD an
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Fig.18~. EKect of TCDD or TCDD and
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1 1.2 l4 Fig. 19b. ENect of TCDD or
Page 168 and 169:
Fig. ZOb. Elkt of TCDD or TCDD and
Page 171 and 172:
Fig. 21. Effect olTCDD or TCDD and
Page 173 and 174:
Fig. 22c. Effect of TCDD or TCDD an
Page 175 and 176:
Fig. 24b. Eflect ofTCDD or TCDD and
Page 177 and 178:
7 REFERENCES Aitken RJ, Irvine DS,
Page 179 and 180:
Chitm KC, Latchoumycandanc,C, Mathu
Page 181 and 182:
Flsher JS, Turner KJ, Brown D, Shar
Page 183 and 184:
Hornsby PJ. Regulation of cytochrom
Page 185 and 186:
Latcboumycandane C, Chitra KC, Math
Page 187 and 188:
Moore RW, Panona JA, Bookstaff RC,
Page 189 and 190:
Rommerts FFG, Cooke BA, Van Der Kem
Page 191 and 192:
Slaunwhite WR, Burgett MJ. h virro
Page 193 and 194:
1.1 Preparation of dosing solution
Page 195 and 196:
1.4.6 Wash concentrate One bottle,
Page 197 and 198:
1.7.3 Estradiol controls Two vials.
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1.1 1.2 Orcinol(6%) 6 g of orcinol
Page 201 and 202:
1.16.2 Horseradish peroxidase (8 Un
Page 203 and 204:
1.20 Superoxide dismutase 1.20.1 Tr
Page 205 and 206:
1.24.2 Thiobarbituric acid (TBA; 0.
Page 207 and 208:
Chronic effect: of enclosulfan on t
Page 209:
enbw1h1.W mu IUY Wcab unpiliml~t at
Page 212 and 213:
cbniuis 11~11 b"nci:~lc ~wlive oxyg
Page 214 and 215:
DNA, RNA ;uxl Wcin wcrr abarval, wh
Page 216 and 217:
Mt~~m(10-lZsu&)vacob- Wfmn Ihehml ~
Page 218 and 219:
New Delhi, llrliil Ib SCI~KK Rcm& M
Page 220 and 221:
tiii~lc rcprmluction or rut (Cllitr
Page 222 and 223:
wciglit (b.wt.) per day for 45 days
Page 224 and 225:
0.1 00 01 " 0.8 I . .lo Dl* rcnn IW
Page 226 and 227:
Chilr:~. K.C.. L!olnn!myci!~dum. C.
Page 228 and 229:
C. hteboumyeradlos . KC. CMtn . P.P
Page 230 and 231:
I. 1 Ilal 01 2 1.7 # I c I ~ ~ L I
Page 232 and 233:
Fin. L Corrclatwn bclwfcn "perm cou
Page 235:
Iionshii nepmdvctivc mxicity ad upt
Page 240 and 241:
o. Glutathione reductage mg pmWn mg
Page 242 and 243:
IOWAME -. Tor. #558517 PME: 9 SESS:
Page 244 and 245:
JOBNAME: Rqm. Tm. M5W7 PAOB: 11 SBS
Page 246 and 247:
t 02 ELSEVIER Toricology OM) (2W1)
Page 248 and 249:
---- - wcre obtu~ncd from E-Monk. G
Page 250:
3.2. A,III~AILNI syrlo~t of ,nIroch
Page 253 and 254:
Uc,#!dy. SC. N;tdcru. S , 1% Conlrb
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