ON TESTIS AND EPlDlDYMlS OF RATS - Pondicherry University ...

1.3.4 Antibody-Enzyme Conjugate solution
One amber vial, 0.3 mL, contained anti-FSH antibody conjugated to the
enzyme horse+adish peroxidase in a protein-based buffer with a non-mercury
preservative. The Antibody-Enzyme Conjugate concentrate was diluted at a
ratio of 1 part into 50 parts of the assay buffer.
1.3.5 Tctramethylbenzidine Chromogen solution (TMB)
One amber bottle, 11 mL contained a solution of TNIB in citrate buffer with
hydrogen peroxide.
1.3.6 Wash concentrate
One bottle, 105 mL contained buffered saline with a nonionic detergent.
Diluted 10-fold with deionized water prior to use.
1.4.1 Anti-LH-Coated Microtitration strips
One strip holder contained 96 microtitration wells coated with anti-LH
antibody. Stored at 2-8°C in the resealable pouch with a desiccant to protect
from moisture.
1.4.2 LH standards
One vial, 1 mL, labeled A contained 0 mlU/ mL and five vials, 0.5 mL each,
labeled B-F, contained concentrations of approximately I, 3, 10, 30 and 100
mlU/ mL LH equine serum with a non-mercury preservative.
1.4.3 LH controls
Two vials, 0.5 mL each. Levels I and 11, contained low and high
concentrations of LH in equine serum matrix with a non-preservative.
1.4.4 Antibody-Enzyme Conjugate solution
One amber vial, 0.3 mL, contained anti-LH antibody conjugated to the enzyme
horseradish peroxidase in a protein-based buffer with a non-mercury
preservative. The Antibody-Enzyme Conjugate concentrate was diluted at a
ratio of 1 part into 50 parts of the assay buffer.
1.4.5 Ternethylbemidine Chromogen solution (TMB)
One amber bottle, 11 mL contained a solution of TMB in citrate buffer with
hydrogen peroxide.