ON TESTIS AND EPlDlDYMlS OF RATS - Pondicherry University ...

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tiii~lc rcprmluction or rut (Cllitru ct HI., 1999: Suj:ltl181 ct ill.. 2(K)I: Cliilnl Ct el.. 2001). Onc of lllc mtist ptcnt cnvironmcnlnl contaminants is 2..1.7.R-tctr;1chlo1~dibenzo-p-tlioxi11 (TCDD). wl~iclis Ihrnicd ;IS ii by.ptoducl in the manuracrllrc ol'chlorini~lcd Iiydrmrbons. This compound is :!lso Fcirnicd in the incineration p m of niu. ~~iciliill ri3i~slc, pilpcr and pulp hlcilching, cmissio~~ Ijt)lll \Iccl li~~~~iilrics i~nd lnotor vcliiclcs (Skc~ic ct ill.. 1'189). Tlie lipphilicily and low rate or rncli~h~ilism of TCDD leads to its accumuhtion ;lncI ~~rsistc~icc in udipor tissues (Enan et HI.. I0'18) It II;IS lrcl~ rcptirtd 1Iii11 niillc rills cxpml to TCI)D displ;~y rcditccd fcrtility, dcliiyed pebcrly ;lnJ altcrcd rcprductivc organ weights (Cri~y ct ;~l.. 1997). Millc MIS cxpoxd to TCDD sllt~wcil dwrc;~snl spcrln cou~irs (Gray ct ill.. 1997) ~IIIJ IIII ~I~L~ICCJ numbcr or ubnonnsl svrlli (I::~qi ct ill.. 1997). Scvec~l niwhi~nis~ns llilvc Iwcn prop~rcd to cxplilin llic toxicity or TCI)I) i111c1 irs coIigcncn (SIR. 1990). Thc most co~isis~cnt biwhcmic;~l cnht or TCDD hi~r been sliown lo be the induction of cytcchrome P450 iasax~tll~tl nnlonnoxygcnas ilnd rcletcd protcin (Pal:~~~il i~nil Kimbrough. 1984). Reantly nryl I~ydrwi~rbon (Ah) rwplor mdinled xnnrliinc ox. idi~x and xunthine dchydrogcnasc system has been rclated lo oxidative stma in vivo in the TCDD.tra11cd rats (Sugiharn et nl.. 2001). Acule highdore exposure to TCDD results in oxidative strcss in mulriplc tissua and species (Mohammnd. pur CI al.. 1988). TCDD has ken nporled to indacc suproxidc anion, lipid peroaidution und DNA damage in the hepai~ and brain lhua of rat (Hassoun et al.. 2001). Mitochondrial and niicros
I kl
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FUNCTIONAL STUDIES ON THE EFFECTS O
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ACKNOWLEDGEMENTS I wish to express
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TABLE OF CONTENTS Page No ACKNOWLED
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TABLE OF CONTENTS (Continued) Deter
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TABLE OF CONTENTS (Continued) Effec
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TAULIC OF CON'I'ISN'I'S (C'untinued
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xi LIST OF TABLES (Continued) I'agc
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Table 24a Table 24b Table 24c Table
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Fig. 1 Fig. 2 Effect of TCDD on the
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Fig. 12c Fig. 12d Fig. 13 Fig. 14a
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LIST OF FIGURES (Continued) Fig. 23
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LH M PL mg mRNA N AD NADH NADP NADP
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layers, an outer layer of visceral
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tubular epithelium and continually
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lmn~unization of monkeys against FS
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and this reaction is catalyzed by t
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extratubular environment or synthes
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the most active in protein secretio
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1958). A rapid fall in serum testos
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have also been reported in the rats
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Effect of TCDD on heart muscle has
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1.4 Effect of oxidative stress on m
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2 SCOPE OF THE PRESENT STUDY There
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3 MATERIALS AND METHODS 3.1 Animals
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Subgroup III: Administration of TCD
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choppcd with the help of sharp razo
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anti-FSH antibody. After incubation
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lust sped (500 to 700 rpm) on an or
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3.13.4 Quantitative determinntiun u
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The plates were dried by inverting
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centrifuged at 3,500 g for I5 min o
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3.17 Preparation of tiasue humogena
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supcrnutant was taken. Hlunk was pr
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3.21.4 Assay of glutathione peroxid
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4 RESULTS 4.1 Administration of TCD
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seminiferous tubules was found to b
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fractions of rat testis as compared
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4.1.11.1 Effect of TCDD on the prod
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4.2 Co-administration of TCDD and v
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4.2.8 Effect of co-administration o
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4.2.11.5 Effect of co-administratio
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Table I b. Effect of TCDD on the we
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Table 4. Effect of TCDD on the seru
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Table 7b. Effect of TCDD on the pro
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Table 8c. Effect of TCDD on the lev
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Table 9d. Effect of TCDD on the act
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Table 13. Effect of TCDD on the ant
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Table 15. Effect of TCDD on the lev
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Table 16c. Effcct of TCDD on the an
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Table 18a. Effect of co-administrat
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Table 21. Effect of co-administrati
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Table 24b. Effect of co-administrat
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Table 25c. Effect of co-administrat
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Table 26d. Effect of co-administrat
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Table 30. Effect of co-administrati
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Table 32. Effect of caadrninistrati
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Table 33c. Effcct of co-administrut
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5 DISCUSSION 5.1 Significance of th
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Measurement of weights of the acces
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compartment of the cells (Chainy el
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the reduction in daily sperm produc
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eported in the TCDD-treated rats (M
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lilnction is to ililcrccpt lipid pc
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in the animals administered with TC
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protective effects on serum testost
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suygcstccl that vivamin 1: conld im
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Fig.4~. ENect of TCDD or TCDD and v
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tE W d In w it I I (,OCX) 3s PUB (,
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Fig. 7c. Effect of TCDD or TCDD and
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u 3 f ~ = m w * N O .- ~Slew aew en
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Fig.lOb. ENmt of TCDD or TCDD and v
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Fig. 1Ir Effect of TCDD or TCDD md
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Fig. 14c. Effect of TCDD or TCDD an
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Fig. 16b. Effect of TCDD or TCDD an
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Fig.18~. EKect of TCDD or TCDD and
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1 1.2 l4 Fig. 19b. ENect of TCDD or
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Fig. ZOb. Elkt of TCDD or TCDD and
Page 171 and 172: Fig. 21. Effect olTCDD or TCDD and
Page 173 and 174: Fig. 22c. Effect of TCDD or TCDD an
Page 175 and 176: Fig. 24b. Eflect ofTCDD or TCDD and
Page 177 and 178: 7 REFERENCES Aitken RJ, Irvine DS,
Page 179 and 180: Chitm KC, Latchoumycandanc,C, Mathu
Page 181 and 182: Flsher JS, Turner KJ, Brown D, Shar
Page 183 and 184: Hornsby PJ. Regulation of cytochrom
Page 185 and 186: Latcboumycandane C, Chitra KC, Math
Page 187 and 188: Moore RW, Panona JA, Bookstaff RC,
Page 189 and 190: Rommerts FFG, Cooke BA, Van Der Kem
Page 191 and 192: Slaunwhite WR, Burgett MJ. h virro
Page 193 and 194: 1.1 Preparation of dosing solution
Page 195 and 196: 1.4.6 Wash concentrate One bottle,
Page 197 and 198: 1.7.3 Estradiol controls Two vials.
Page 199 and 200: 1.1 1.2 Orcinol(6%) 6 g of orcinol
Page 201 and 202: 1.16.2 Horseradish peroxidase (8 Un
Page 203 and 204: 1.20 Superoxide dismutase 1.20.1 Tr
Page 205 and 206: 1.24.2 Thiobarbituric acid (TBA; 0.
Page 207 and 208: Chronic effect: of enclosulfan on t
Page 209: enbw1h1.W mu IUY Wcab unpiliml~t at
Page 212 and 213: cbniuis 11~11 b"nci:~lc ~wlive oxyg
Page 214 and 215: DNA, RNA ;uxl Wcin wcrr abarval, wh
Page 216 and 217: Mt~~m(10-lZsu&)vacob- Wfmn Ihehml ~
Page 218 and 219: New Delhi, llrliil Ib SCI~KK Rcm& M
Page 222 and 223: wciglit (b.wt.) per day for 45 days
Page 224 and 225: 0.1 00 01 " 0.8 I . .lo Dl* rcnn IW
Page 226 and 227: Chilr:~. K.C.. L!olnn!myci!~dum. C.
Page 228 and 229: C. hteboumyeradlos . KC. CMtn . P.P
Page 230 and 231: I. 1 Ilal 01 2 1.7 # I c I ~ ~ L I
Page 232 and 233: Fin. L Corrclatwn bclwfcn "perm cou
Page 235: Iionshii nepmdvctivc mxicity ad upt
Page 240 and 241: o. Glutathione reductage mg pmWn mg
Page 242 and 243: IOWAME -. Tor. #558517 PME: 9 SESS:
Page 244 and 245: JOBNAME: Rqm. Tm. M5W7 PAOB: 11 SBS
Page 246 and 247: t 02 ELSEVIER Toricology OM) (2W1)
Page 248 and 249: ---- - wcre obtu~ncd from E-Monk. G
Page 250: 3.2. A,III~AILNI syrlo~t of ,nIroch
Page 253 and 254: Uc,#!dy. SC. N;tdcru. S , 1% Conlrb
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