ON TESTIS AND EPlDlDYMlS OF RATS - Pondicherry University ...

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Table 13. Effect of TCDD on the antioxidant enzymes and lipid peroxidation in the epididymal sperm of adult rats TCDDI Kg body weight Control I ng 10 ng I00 ng Superoxide dismutase' (mg protein) 22.405 1.43 20.30i2.85' 18.50i 1.51. 16.90k 1.57' (mg DNA) 18.08+ 0.61 13.64+ 1.14' 12.48i 0.72* 11.385 1.22* catalaseb (ing protein) 2.49f 0.13 2.33 i 0.15' 2.305 0.14' 2.03 i0.13' (mg DNA) 2.01 i 0.05 1.45 i 0.06. 1.44 + 0.08* 1.35 + 0.05' Glutathione reductase' (mg protein) 40.00* 2.17 31.20f 2.41. 28.20i 2.49* 27.10f 1.57' (mg DNA) 32.41 f 0.86 19.46i 0.53. 20.l8i 0.83' 18.07i 0.76* Glutathione peroxidase' (mg protein) 71.20i 3.87 64.50i 4.88' 56.605 4.41. 48,OOi 3.97. (mg DNA) 57.58 i 1.52 40.17 i 1.05' 38.24 i 0.88. 31.94 + 1.79. Lipid peroxidatton' (trig protein) 2.17 i 0.20 4.24 i0.418 4.80f 0.38' 6.08+ 0.20' (mg DNA) 1.75 + 0.82 2.W+ 0.13. 3.24i 0.08' 4.05i 0.12. nlnol/ tninl mg protein or DNA pmoll minl rng protein or DNA moll mg protein or DNA The data are expressed as meaniSD for six animals! dose *p
Table 14. Effect of TCDD on the production of superoxide anion, nitric oxide and hydrogen peroxide in the caput, corpus and cauda epididyrnides of adult rats. TCDDl Kg body weight Control I ng 10 ng I00 ng Cuput cl~ididyrrri.~ Superoxide anion' 2.01 i 0.41 2.1 1 1 0.1 I* 2.91 f 0.17. 3.14 * 0.31- Nitric oxideb 1.46-t 0.13 2.66-t 0.41. 2.71 f 0.34' 2.93 * 0.23* Hydrogen peroxideb 11.52 * 1.66 19.98 f 3.31' 36.25 + 2.05' 75.42 k 5.7IL Superoxide anion0 1.31 i 0.30 2.49* 0.10' 2.71 f 0.19' 3.01 i 0.17. Nitric oxideb 1.36* 0.21 2.01 f 0.18. 2.46-t 0.41' 2..91 i 0.14' Hydrogen peroxideb 20.50 f 1.68 37.70 13.87' 58.25 2 6.17: 109.6 i 19.3' Superoxide anionn 2.07 + 0.2 I 2.71 + 0.26. 2.97 i 0.40. 3.29 i 0.14. Nirric oxideb 1.91 -t 0.33 2.61 + 0.40. 2.93 + 0.17' 3.40* 0.16. tlydrogen peroxideb 15.76 f 1.40 20.11 i 1.42, 31.86 i 3.43. 52.8 + 5.28* nrnoll mid rng protein; mmoll mid mg protein nrnol Hz02 generatedl mid mp protein The number of animals per group is six 'pc0.05 against the control group
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FUNCTIONAL STUDIES ON THE EFFECTS O
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ACKNOWLEDGEMENTS I wish to express
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TABLE OF CONTENTS Page No ACKNOWLED
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TABLE OF CONTENTS (Continued) Deter
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TABLE OF CONTENTS (Continued) Effec
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TAULIC OF CON'I'ISN'I'S (C'untinued
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xi LIST OF TABLES (Continued) I'agc
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Table 24a Table 24b Table 24c Table
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Fig. 1 Fig. 2 Effect of TCDD on the
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Fig. 12c Fig. 12d Fig. 13 Fig. 14a
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LIST OF FIGURES (Continued) Fig. 23
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LH M PL mg mRNA N AD NADH NADP NADP
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layers, an outer layer of visceral
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tubular epithelium and continually
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lmn~unization of monkeys against FS
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and this reaction is catalyzed by t
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extratubular environment or synthes
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the most active in protein secretio
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1958). A rapid fall in serum testos
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have also been reported in the rats
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Effect of TCDD on heart muscle has
Page 43 and 44: 1.4 Effect of oxidative stress on m
Page 45 and 46: 2 SCOPE OF THE PRESENT STUDY There
Page 47 and 48: 3 MATERIALS AND METHODS 3.1 Animals
Page 49 and 50: Subgroup III: Administration of TCD
Page 51 and 52: choppcd with the help of sharp razo
Page 53 and 54: anti-FSH antibody. After incubation
Page 55 and 56: lust sped (500 to 700 rpm) on an or
Page 57 and 58: 3.13.4 Quantitative determinntiun u
Page 59 and 60: The plates were dried by inverting
Page 61 and 62: centrifuged at 3,500 g for I5 min o
Page 63 and 64: 3.17 Preparation of tiasue humogena
Page 65 and 66: supcrnutant was taken. Hlunk was pr
Page 67 and 68: 3.21.4 Assay of glutathione peroxid
Page 69: 4 RESULTS 4.1 Administration of TCD
Page 72 and 73: seminiferous tubules was found to b
Page 74 and 75: fractions of rat testis as compared
Page 76 and 77: 4.1.11.1 Effect of TCDD on the prod
Page 78 and 79: 4.2 Co-administration of TCDD and v
Page 80 and 81: 4.2.8 Effect of co-administration o
Page 82 and 83: 4.2.11.5 Effect of co-administratio
Page 84 and 85: Table I b. Effect of TCDD on the we
Page 86 and 87: Table 4. Effect of TCDD on the seru
Page 88 and 89: Table 7b. Effect of TCDD on the pro
Page 90 and 91: Table 8c. Effect of TCDD on the lev
Page 92 and 93: Table 9d. Effect of TCDD on the act
Page 96 and 97: Table 15. Effect of TCDD on the lev
Page 98 and 99: Table 16c. Effcct of TCDD on the an
Page 100 and 101: Table 18a. Effect of co-administrat
Page 103 and 104: Table 21. Effect of co-administrati
Page 105 and 106: Table 24b. Effect of co-administrat
Page 107 and 108: Table 25c. Effect of co-administrat
Page 109 and 110: Table 26d. Effect of co-administrat
Page 111 and 112: Table 30. Effect of co-administrati
Page 113 and 114: Table 32. Effect of caadrninistrati
Page 115 and 116: Table 33c. Effcct of co-administrut
Page 117 and 118: 5 DISCUSSION 5.1 Significance of th
Page 119 and 120: Measurement of weights of the acces
Page 121 and 122: compartment of the cells (Chainy el
Page 123 and 124: the reduction in daily sperm produc
Page 125 and 126: eported in the TCDD-treated rats (M
Page 127 and 128: lilnction is to ililcrccpt lipid pc
Page 129 and 130: in the animals administered with TC
Page 131 and 132: protective effects on serum testost
Page 133: suygcstccl that vivamin 1: conld im
Page 136 and 137: Fig.4~. ENect of TCDD or TCDD and v
Page 138: tE W d In w it I I (,OCX) 3s PUB (,
Page 142 and 143: Fig. 7c. Effect of TCDD or TCDD and
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u 3 f ~ = m w * N O .- ~Slew aew en
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Fig.lOb. ENmt of TCDD or TCDD and v
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Fig. 1Ir Effect of TCDD or TCDD md
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Fig. 14c. Effect of TCDD or TCDD an
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Fig. 16b. Effect of TCDD or TCDD an
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Fig.18~. EKect of TCDD or TCDD and
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1 1.2 l4 Fig. 19b. ENect of TCDD or
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Fig. ZOb. Elkt of TCDD or TCDD and
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Fig. 21. Effect olTCDD or TCDD and
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Fig. 22c. Effect of TCDD or TCDD an
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Fig. 24b. Eflect ofTCDD or TCDD and
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7 REFERENCES Aitken RJ, Irvine DS,
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Chitm KC, Latchoumycandanc,C, Mathu
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Flsher JS, Turner KJ, Brown D, Shar
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Hornsby PJ. Regulation of cytochrom
Page 185 and 186:
Latcboumycandane C, Chitra KC, Math
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Moore RW, Panona JA, Bookstaff RC,
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Rommerts FFG, Cooke BA, Van Der Kem
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Slaunwhite WR, Burgett MJ. h virro
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1.1 Preparation of dosing solution
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1.4.6 Wash concentrate One bottle,
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1.7.3 Estradiol controls Two vials.
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1.1 1.2 Orcinol(6%) 6 g of orcinol
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1.16.2 Horseradish peroxidase (8 Un
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1.20 Superoxide dismutase 1.20.1 Tr
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1.24.2 Thiobarbituric acid (TBA; 0.
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Chronic effect: of enclosulfan on t
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enbw1h1.W mu IUY Wcab unpiliml~t at
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cbniuis 11~11 b"nci:~lc ~wlive oxyg
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DNA, RNA ;uxl Wcin wcrr abarval, wh
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Mt~~m(10-lZsu&)vacob- Wfmn Ihehml ~
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New Delhi, llrliil Ib SCI~KK Rcm& M
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tiii~lc rcprmluction or rut (Cllitr
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wciglit (b.wt.) per day for 45 days
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0.1 00 01 " 0.8 I . .lo Dl* rcnn IW
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Chilr:~. K.C.. L!olnn!myci!~dum. C.
Page 228 and 229:
C. hteboumyeradlos . KC. CMtn . P.P
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I. 1 Ilal 01 2 1.7 # I c I ~ ~ L I
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Fin. L Corrclatwn bclwfcn "perm cou
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Iionshii nepmdvctivc mxicity ad upt
Page 240 and 241:
o. Glutathione reductage mg pmWn mg
Page 242 and 243:
IOWAME -. Tor. #558517 PME: 9 SESS:
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JOBNAME: Rqm. Tm. M5W7 PAOB: 11 SBS
Page 246 and 247:
t 02 ELSEVIER Toricology OM) (2W1)
Page 248 and 249:
---- - wcre obtu~ncd from E-Monk. G
Page 250:
3.2. A,III~AILNI syrlo~t of ,nIroch
Page 253 and 254:
Uc,#!dy. SC. N;tdcru. S , 1% Conlrb
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