ON TESTIS AND EPlDlDYMlS OF RATS - Pondicherry University ...

1.20 Superoxide dismutase
1.20.1 Tris HCI buffer (50 mM; pH 8.2)
605.7 mg of Tris HCI was dissolved in I00 mL of distilled water (50 mM). To
this solution 0.0372 g of EDTA was added and pH was adjusted to 8.2.
12.6 mg of pyrogallol was dissolved in 50 mL of I0 mM HCI.
1.20.3 10 mM HCI
0.0416 mL or 41.6 pL of coocentrated HCI was made up to 50 mL in a
standard flask.
1.21 Catalase
1.2 1.1 Phophate buffer (0.0SM; pH 7.0)
0.89025 g of disodium hydrogen phosphate dihydrate was dissolved in 100
mL of distilled water (Solution A).
0.691 75 g of sodium dihydrogen phosphate monohydrate was dissolved in 100
mL of distilled water (Solution B).
Mixed 39 mL of solution A with 61 mL of solution B and made upto 200 mL
with distilled water and then pH was adjusted to 7.0.
1.21.2 Hydrogen peroxide (0.019M)
58pL of 30% hydrogen peroxide was made up to 100 mL with distilled water
and stored in cool and dark place.
1.22 Glutathione reductnse
1.22.1 Phosphate buffer (0.1 M, pH 7.6)
1.78 g of disodium hydrogen phosphate was dissolved in 100 mL of distilled
water (Solution A).
13.835 g of sodium dihydrogen phosphate was dissolved in 100 mL of
distilled water (Solution B).
13 mL of solution A and 87 mL of solution B was taken and diluted to 200 mL
and pH was adjusted to 7.6.