ON TESTIS AND EPlDlDYMlS OF RATS - Pondicherry University ...
ON TESTIS AND EPlDlDYMlS OF RATS - Pondicherry University ...
ON TESTIS AND EPlDlDYMlS OF RATS - Pondicherry University ...
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ELSEVIER<br />
Toricology OM) (2W1) DM-wo<br />
wwv.el~vicr.com/lo~~le~loii~)l<br />
Effect of methoxychlor on the antioxidant system in<br />
mitochondrial and microsome-rich fractions of rat testis<br />
C. Latchoumycandane, P.P. Mathur I*<br />
S'hwl v/ LII Scicnrrr. Pvldtchrrry Un~urriry. Pondrdcrry 605 014, Indra<br />
Rccctvcd 21 N~vcmbcr ZmI, rccetvcd m rcvn~d iorm 19 h m h r 2001. a~cc~~cd 28 March 2W1<br />
Abstract<br />
Mcthoaychior. an cnvironmenlal contdmlnanl, which is widely used as a pesticide in many countries and has k n<br />
shown lo ~nducc rcproductivc abnormalities ~n mule rats The precise nature and mechanism ot action of methoxy<br />
cllior on lllc lniblc rcproducllve ryslcm is no1 clwr In tho prcxnt study, we have sought to invesligille the induction<br />
of o*ldat~vc stress In thc tesl~l of rat after expoure to methorychlor Melhoiychlor (I, 10, and IW mg kg-' body<br />
wcnghl wr Jsy) was adrninisttrsd ormlly to the rat for 45days Aitcr 24 h of the last treatment the anlmals were killed<br />
wing :~ne\.thct~c clhcr. The body welght of thc anlmalr adm~n~slcrcd with methorychlor d~d not show any s~gn~flcam<br />
chungc Thc wcaghts of the testis, cpadtdymis, seminal vn!cles and ventral prostate dscreawd significantly in 100 mg<br />
dox but rcma~ncd unchsngtd In I and 10 ma do~s<br />
Miloehondrial and microsome-rich fract~ons of the test~s were<br />
oblatned by the method or d~llcrcntlal centrifugation. The activitles of anttoridant enzymes such as superoxide<br />
dlsmutasc. cslnlarc, plutath~one rcducmre and glutnthlone prox*dasc decreued s~gnificantly ,n the animals treated<br />
well8 tnc~horyci~lor In a dose-dependent manner In thc m~lochondilal and micromme-rich fraetloer of rat testis The<br />
lcvclr of hydrogcn peroiidc pncmllon (H,OI) and lip~d peroxtdatlon increased in m~~ochondriul and m~crorome-rich<br />
lracl~ons of the lcstts of the rats tmtd with melhoxychlor The rcsulu suppaled that the low to medlum dosn ol<br />
rncthuxyci,lor clcn deplet8on of antloxtdanl enzymes and concom~lant increase In the levels or HIO* and lipid<br />
prortdiltian dtllcrcnl\ally in mitochondrial and micro~omc-nch fractions of rat testis In conclusion, the adverse<br />
cflccl 01 mcthoxychlor on malc rsproducuon could be duc to lhc lnduclion of oxidal~ve stress in lestis. Q 2002<br />
Publl,hd by Elrvicr Sclsncc Ireland Ltd<br />
lcywvrdr Mc~horychior, Tuur, Anboridanl cnqmn. L~ptd pcrondalaon. Mllochondnsl fnclionr, Mncroromcrich Irlclionl: Rat<br />
The exposure of humans to cnvironmcntal contaminants<br />
that negatlvely affccl the male reproductive<br />
svstcm is increasing -(Sham, . 1993: Chia,<br />
'Cormpondlny author Tel. +91413615.211, 81. +91. 2000,, 11;~ important to notc that many cnviron.<br />
413.bJJ.lll<br />
K.n~~a1~~d~,rcu pp~~~;~~I~ut~~!l~olm~~I,com<br />
(P.P Muthur) mcnta' like organochlor'ne<br />
' B.mwl vpnnlhurgpl pon.n~.tn, cider, accumulate in fatty tissues. Therefore,