ON TESTIS AND EPlDlDYMlS OF RATS - Pondicherry University ...

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eduction in Leydig cell volume per testis (Wilker, 1996). Reduction in the weight ol' the testis could be due to the Loss of germ cells by direct inhibition of TCDD on spermatogenesis or an indirect effect through inhibition of testosterone production. The present findings are consistent with previous report in which reduction in weight of the testis may be due to the loss of germ cells and Sertoli cells in the TCDD-treated rats (Mably el al., 1992; Johnson el ul., 1992). The weights of epididymis, seminal vesicles and ventral prostate decreased significantly in adult rats while weight of the kidney remained unchanged following administration of TCDD (Fig. 4b to 4f; Table la & I b). Epididyrnis and accessory sex organs require a continuous androgenic stimulation for their normal growth and functions (Klinefelter and Hess, 1998). It has been reported that accessory sex organs are androgen dependent and thus reflect the availability of androgen over a period of time (Mathw and Chanopadhyay. 1982). TCDD has also been reported to reduce plasma testosterone and dihydrotestosterone in the rats (Mably er ul., 1992). Inhibition on the weights of the epididymis, seminal vesicles and ventral prostate may be either due to the reduction in the levels of serum testosterone or a direct action of TCDD on the epididymis and accessory sex organs. 'She observed reduction in thu weight of accessory sex organs also reflected reduced bioavailability andl or production of androgen in the TCDD-treated rats. In the present studies administration of TCDD at these doses did not affect the weights of the extra- reproductive tissues like kidney though the activity of P-gluco~nidase in kidney has been demonstrated as testosterone dependent enzyme (Malarvizhi and Mathur, 1996). 5.2.2 Effect oCTCDD oo daily spcrm production Adn>inistntion of TCDD dccrcascd siynilicunlly thc doily spcrm production as compared to the corresponding groups of control animals (Fig. 5a: Table 2). Thc observed reduction in the daily sperm production also rellects reduction in spermatogenesis. The present findings are consistent with the earlier reports in which
the reduction in daily sperm production has been shown in TCDI>-treated rats (Sommer el ul., 1996; Faqi el ul., 1997; 'l'hcohald and I'ctorson, 1997). 5.2.3 Effect of TCDD on epididymal sperm viability, motility and count The epididymal sperm viability remained unchanged while the epididymal sperm motility decreased significantly in a dose-dependent manner in the animals administered with TCDD (Fig. 5a & 5b; Table 2). Administration of TCDD decreased epididymal sperm count significantly when compared to the corresponding groups of control animals (Fig. Sa; Table 2). The present findings are consistent with the earlier report in which the reduction in daily sperm production and epididymal sperm count in the rats treated with TCDD (Sommer el al.. 1996; Faqi el al., 1997; Theobald and Peterson, 1997). 5.2.4 Effect of TCDD on seminiferous tubular and lumen diameter Diameter of the seminiferous tubular and lumen decrcased signilicantly alicr administration of TCDD as compared to the corresponding groups of control animals (Fig. 6; Table 3). The reduction in the tubular and lumen diameter of' thc seminiferous tubules may be due to a direct action of TCDD on the seminiferous tubules or an indirect effect through inhibition of testosterone production. The reduction in the tubular diameter has been correlated with decreased spermatogenic activity of the testis (Sinha Hikim cl a/., 1989). 5.2.5 EfTcct of TCDD on the levels of serum horlnoi~cs and stcrc~idogei~ic enzymes in testis The levels of serum testosterone and rstradiol were found lo be decreased in the TCDD-treated rats along with reduction in the activities of 3P-hydroxysteroid dehydrogenase and I7p-hydroxysteroid dehydrogenase in testis (Fig. 7a to 7e & 8: Table 4 & 5). A decrease in the intratesticular and serum testosterone concentration has been reported in rats following administration of TCDD (Wilker. 1996). It has
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FUNCTIONAL STUDIES ON THE EFFECTS O
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ACKNOWLEDGEMENTS I wish to express
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TABLE OF CONTENTS Page No ACKNOWLED
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TABLE OF CONTENTS (Continued) Deter
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TABLE OF CONTENTS (Continued) Effec
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TAULIC OF CON'I'ISN'I'S (C'untinued
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xi LIST OF TABLES (Continued) I'agc
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Table 24a Table 24b Table 24c Table
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Fig. 1 Fig. 2 Effect of TCDD on the
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Fig. 12c Fig. 12d Fig. 13 Fig. 14a
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LIST OF FIGURES (Continued) Fig. 23
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LH M PL mg mRNA N AD NADH NADP NADP
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layers, an outer layer of visceral
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tubular epithelium and continually
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lmn~unization of monkeys against FS
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and this reaction is catalyzed by t
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extratubular environment or synthes
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the most active in protein secretio
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1958). A rapid fall in serum testos
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have also been reported in the rats
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Effect of TCDD on heart muscle has
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1.4 Effect of oxidative stress on m
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2 SCOPE OF THE PRESENT STUDY There
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3 MATERIALS AND METHODS 3.1 Animals
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Subgroup III: Administration of TCD
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choppcd with the help of sharp razo
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anti-FSH antibody. After incubation
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lust sped (500 to 700 rpm) on an or
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3.13.4 Quantitative determinntiun u
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The plates were dried by inverting
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centrifuged at 3,500 g for I5 min o
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3.17 Preparation of tiasue humogena
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supcrnutant was taken. Hlunk was pr
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3.21.4 Assay of glutathione peroxid
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4 RESULTS 4.1 Administration of TCD
Page 72 and 73: seminiferous tubules was found to b
Page 74 and 75: fractions of rat testis as compared
Page 76 and 77: 4.1.11.1 Effect of TCDD on the prod
Page 78 and 79: 4.2 Co-administration of TCDD and v
Page 80 and 81: 4.2.8 Effect of co-administration o
Page 82 and 83: 4.2.11.5 Effect of co-administratio
Page 84 and 85: Table I b. Effect of TCDD on the we
Page 86 and 87: Table 4. Effect of TCDD on the seru
Page 88 and 89: Table 7b. Effect of TCDD on the pro
Page 90 and 91: Table 8c. Effect of TCDD on the lev
Page 92 and 93: Table 9d. Effect of TCDD on the act
Page 94 and 95: Table 13. Effect of TCDD on the ant
Page 96 and 97: Table 15. Effect of TCDD on the lev
Page 98 and 99: Table 16c. Effcct of TCDD on the an
Page 100 and 101: Table 18a. Effect of co-administrat
Page 103 and 104: Table 21. Effect of co-administrati
Page 105 and 106: Table 24b. Effect of co-administrat
Page 107 and 108: Table 25c. Effect of co-administrat
Page 109 and 110: Table 26d. Effect of co-administrat
Page 111 and 112: Table 30. Effect of co-administrati
Page 113 and 114: Table 32. Effect of caadrninistrati
Page 115 and 116: Table 33c. Effcct of co-administrut
Page 117 and 118: 5 DISCUSSION 5.1 Significance of th
Page 119 and 120: Measurement of weights of the acces
Page 121: compartment of the cells (Chainy el
Page 125 and 126: eported in the TCDD-treated rats (M
Page 127 and 128: lilnction is to ililcrccpt lipid pc
Page 129 and 130: in the animals administered with TC
Page 131 and 132: protective effects on serum testost
Page 133: suygcstccl that vivamin 1: conld im
Page 136 and 137: Fig.4~. ENect of TCDD or TCDD and v
Page 138: tE W d In w it I I (,OCX) 3s PUB (,
Page 142 and 143: Fig. 7c. Effect of TCDD or TCDD and
Page 145 and 146: u 3 f ~ = m w * N O .- ~Slew aew en
Page 147 and 148: Fig.lOb. ENmt of TCDD or TCDD and v
Page 151: Fig. 1Ir Effect of TCDD or TCDD md
Page 158 and 159: Fig. 14c. Effect of TCDD or TCDD an
Page 160 and 161: Fig. 16b. Effect of TCDD or TCDD an
Page 164 and 165: Fig.18~. EKect of TCDD or TCDD and
Page 166 and 167: 1 1.2 l4 Fig. 19b. ENect of TCDD or
Page 168 and 169: Fig. ZOb. Elkt of TCDD or TCDD and
Page 171 and 172: Fig. 21. Effect olTCDD or TCDD and
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Fig. 22c. Effect of TCDD or TCDD an
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Fig. 24b. Eflect ofTCDD or TCDD and
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7 REFERENCES Aitken RJ, Irvine DS,
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Chitm KC, Latchoumycandanc,C, Mathu
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Flsher JS, Turner KJ, Brown D, Shar
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Hornsby PJ. Regulation of cytochrom
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Latcboumycandane C, Chitra KC, Math
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Moore RW, Panona JA, Bookstaff RC,
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Rommerts FFG, Cooke BA, Van Der Kem
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Slaunwhite WR, Burgett MJ. h virro
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1.1 Preparation of dosing solution
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1.4.6 Wash concentrate One bottle,
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1.7.3 Estradiol controls Two vials.
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1.1 1.2 Orcinol(6%) 6 g of orcinol
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1.16.2 Horseradish peroxidase (8 Un
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1.20 Superoxide dismutase 1.20.1 Tr
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1.24.2 Thiobarbituric acid (TBA; 0.
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Chronic effect: of enclosulfan on t
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enbw1h1.W mu IUY Wcab unpiliml~t at
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cbniuis 11~11 b"nci:~lc ~wlive oxyg
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DNA, RNA ;uxl Wcin wcrr abarval, wh
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Mt~~m(10-lZsu&)vacob- Wfmn Ihehml ~
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New Delhi, llrliil Ib SCI~KK Rcm& M
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tiii~lc rcprmluction or rut (Cllitr
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wciglit (b.wt.) per day for 45 days
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0.1 00 01 " 0.8 I . .lo Dl* rcnn IW
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Chilr:~. K.C.. L!olnn!myci!~dum. C.
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C. hteboumyeradlos . KC. CMtn . P.P
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I. 1 Ilal 01 2 1.7 # I c I ~ ~ L I
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Fin. L Corrclatwn bclwfcn "perm cou
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Iionshii nepmdvctivc mxicity ad upt
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o. Glutathione reductage mg pmWn mg
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IOWAME -. Tor. #558517 PME: 9 SESS:
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JOBNAME: Rqm. Tm. M5W7 PAOB: 11 SBS
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t 02 ELSEVIER Toricology OM) (2W1)
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---- - wcre obtu~ncd from E-Monk. G
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3.2. A,III~AILNI syrlo~t of ,nIroch
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Uc,#!dy. SC. N;tdcru. S , 1% Conlrb
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