ON TESTIS AND EPlDlDYMlS OF RATS - Pondicherry University ...

fractions of rat testis as compared to the corresponding groups of control animals
(Table 812).
4.1.10 Effect of TCDD on the antioxidant enzymes and lipid pcroxidation in
crude homogenate, mitochondrial and microsome-rich fractions of testis
The activity of superoxide dismutase decreased significantly in the crude
homogenate as well as in the mitochondrial and microsome-rich fractions of rat testis
as compared to the corresponding group of control animals (Table 9a).
The activity of catalase decreased significantly in the crude homogenate as
well as in the mitochondrial and microsome-rich fractions of rat testis as compared to
the corresponding group of control animals (Table 9b).
The activity of glutathione reductase decreased significantly in the crude
homogenate as well as in the mitochondrial and microsome-rich fractions of rat testis
as compared to the corresponding group of control animals (Table 9c).
The activity of glutathione peroxidase decreased significantly in the crude
homogenate as well as in the mitochondrial and microsome-rich fractions of rat testis
as compared to the corresponding group of control animals (Table 9d).
The level of lipid peroxidation increased significantly in the crude homogenate
as well as in the mitochondria1 and microsome-rich fractions of rat testis as compared
to the corresponding group of control animals (Table 10).
4.1.1 1 Effcct of TCDD on the cpididymnl spcrm and cpididymis
002661.
A positive correlation (r = 0.95; n = 24) was observed between sperm counts
and DNA content in the epididymal sperm (Fig. 2). The DNA contents were routinely
used as an indicator of sperm counts. The production of reactive oxygen species.
levels of antioxidants, activities of antioxidant enzymes and lipid peroxidation were
expressed both in terms of protein and DNA.