ON TESTIS AND EPlDlDYMlS OF RATS - Pondicherry University ...

1.17.4 Disodium hydrogen phosphate (0.3 M)
5.34 g of disodium hydrogen phosphate was dissolved in I00 tnL of distilled
water.
1.17.5 Glutathione standard
100 mg of glutathione reduced in 100 mL distilled water and stored as
working standard. Stock was diluted to get a concentration of 100 pd mL.
1 .I 8.1 2,Z'-Dipyridyl solution (0.2%)
0.2 g of dipyridyl was dissolved in I00 mL of ethanol.
1 .I 8.2 Ferric chloride solution (0.5%)
0.5 g of ferric chloride was dissolved in 100 mL of ethanol.
1.18.3 a-Tocopherol (standard)
100 mg of DL-tocopherol was dissolved in 100 mL of distilled ethanol. Stock
solution was diluted to get a concentration of 10 pgl mL.
1.19 Ascorbic acid
1.19.1 Metaphosphoric acid (2.5%)
2.5 g of mctaphosphoric acid was dissolved in 100 mL of distilled water.
1.19.2 Dichlorophenol indophenol acetate
7.5 mg of dichlorophenol indophenol was dissolved in 100 mL of distilled
water at 85OC, filtered hot, cooled and diluted to 250 mL (Solution A).
4.5309 g of sodium acetate was dissolved in 100 mL of distilled water and pH
was adjusted to 7.0 with 0.5 M acetic acid (solution B).
Mixed equal volume of solution A and Solution B at the time of use
1.19.3 Ascorbic acid (standard)
100 mg of ascorbic acid was dissolved in 100 mL distilled water and stored as
working standard. Stock was diluted to get a concentration of 100 pgl mL.