ON TESTIS AND EPlDlDYMlS OF RATS - Pondicherry University ...

1.4.6 Wash concentrate
One bottle, 105 mL contained buffered saline with a nonionic detergent.
Diluted 10-fold with deionized water prior to use.
1.5.1 Anti-Prolactin-Coated microtitration strips
One strip holder contained 96 microtitration wells coated with anti-prolactin
antibody. Stored at 2-8°C in the resealable pouch with a desiccant to protect
from moisture.
1.5.2 Prolactin standards
One vial, labeled A contained 0 ngl rnL and five vials, labeled B-F, contained
concentrations of approximately 2, 6, 20, 60 and 180 ngl mL prolactin in
porcine serum with a non-mercury preservative.
1.5.3 Prolactin serum controls
Two vials, Levels I and 11, contained low and high concentrations of prolactin
in porcine serum with a non-mercury preservative.
1.5.4 Antibody-Enzyme Conjugate solution
One vial, 0.3 mL, contained anti-prolactin antibody conjugated to the enzyme
horseradish peroxidase in a protein-based (BSA) buffer with a non-mercury
preservative. The Antibody-Enzyme Conjugate concentrate was diluted at a
ratio of 1 part into SO parts of the assay buffer.
1.5.5 Tetramethylbenzidine Chromogen solution (TMB)
One amber bottle, 11 mL contained a solution of TMB in citrate buffer with
hydrogen peroxide.
1.5.6 Wash concentrate
One bottle. 60 mL contained buffered saline with a nonionic detergent. Diluted
25-fold with deionized water prior to use.
1.6 Serum testosterone
1.6.1 GARG-Coated microtitmtion strips
One strip holder contained 96 polystyrene microtitre wells coated with goat
anti-rabbit 1gG immobilized to the inside wall of each well. Stored at 2-S°C in
the resealable pouch with a desiccant to protect from moisture.