ON TESTIS AND EPlDlDYMlS OF RATS - Pondicherry University ...

Subgroup III: Administration of TCDD at the dose of 100 ng/ Kg body
weight1 day.
3.5.2 Group 11: Administration of TCDD along with vitamin E
Vitamin E was dissolved in olive oil (see Appendix 1 .I) at the dose level of 20
mg/ Kg body weight and administered orally to different groups of rats along with
various doses of TCDD. Co-administration of vitamin E and TCDD was done by the
methods as described previously (see Section 3.4.1). Corresponding groups of control
animals were administered with equal volume of vitamin E.
Subgroup I : AdminisIration of vitamin E along with TCDD at the dose of 1
ng/ Kg body weight/ day.
Subgroup I1 : Administration of vitamin E along with TCDD at the dose of
10 ng/ Kg body weight/ day.
Subgroup 111: Administration of vilamin E along with TCDD at the dosc of
100 ng/ Kg body weight/ day.
3.6 Killing of animals
AfIer 24 h of the last treatment the rats were weighed and killed using overdosage
of anesthetic ether.
3.7 Collection of serum
Blood samples were collected in glass centrifuge tubes either by cardiac
puncture method or decapitation and were kept at 4'C for 12 h and then centrifuged at
ZOO0 g for 15 min. Serum samples werc collected and stored at - 20°C i111til IISC~.
3.8 Collection of tissues
Kidney. testes and accessory sex organs (seminal vesicles and ventral prostate)
werc dissated out and washed in cold normal saline. The epididymides were
dissected out and washed in normal saline at mom temperaturn.