ON TESTIS AND EPlDlDYMlS OF RATS - Pondicherry University ...

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Mt~~m(10-lZsu&)vacob- Wfmn Ihehml ~ n i m l H a a o l h ~ ~ & ~ InUilUc d Rnlpdue Medi 6Judion nd Rkirch (JIPMER). hdiehey, Ida. Mmb *as mintnincd in pMic cya u*la 8 dl-qiwd S i d drlr(lZh:lZh)~k*(#t5)~.Sadrd m m r r d a l ~ d n w a d l Q ~ * c n r ~ e d l k . Undnc (5.0 m&hL, h o h dl) wm @vcn dlyMa~of~.Om&!xdyrdgpr&y14 ad. fhcmmmllnimhnoind8rbnibrrdvrrol chevehick. BodywighQmt~oncnyIlcanale dny mil IC ad d Ihe qahml. 'Ibo mhmb ux killal by Uhcr 24 h llla OD Y -. Bpld i vim mval, dcnsd dl Ihe ldahy ligucr md wished. Thc epidi qona a- ad d h y weir. dm bmrd*cty.nd mddq qam vac cdkc4alMduscdfn~m)n.
WnCr, inyminn~nt of spmri motility, nducsd futiliza- dicnlg (hsl lLdan inducts oxidative s w in epididymis licm ability, lbnamal ~pum in mcn and and cpididymal sprm. ?hs significant incresrc in Lhs wildlifd"' . Our @OUS Uudia on onc of be avimn- lipid puoxidation of cpididymal spem in linjarr.tmled maurl mnminanta adosulfan - have shaMl to esusc im- rnts has dm brm ascciaLed with the dsnsscd sprm primant of tentkulur furrcionr Pnd k t the Bndrogmic- mocilily. 'Ihc gcnascion of ROS has bm mrnlated with ityinrsul". AQr&kanlinIhcpcsmtuudy (5.0 f&alogicaI cprm ~ o r as i oligosprmis ~ tanned as ~oflindac/~~bodywdght)h~becnrmridacdas defeaivc spmns' with Rduced cprm motility, futility No Obaaved meu Led (~05)['). Lindsrr treat- Md span-mcyfc In the prrrcnt atudy, tke he. muH haa dmvn a tignirmnt in thc body wight pididymd spam mts and spm motility wn &- ud thc 4ghU of mtia. vcnlnl pmsfate and 6aninal crraccd in IindPnc-treaud rats as cwparod to the mnml ~eoickr('~. Ln thc -I .udicr Ibc epididw wight anhla ilxlbiq thst epididymia was undsrgoing oxidaof lindnne-W IUIO was deacsrd eignifu.nntly and liw rmss. Illis my be due to lk drrravd biavaihbility and produccim of albgas(~~. Ro-oxidon1 onl ~ hiQIidPI1( bdum k viml r~ nornvllbidoplcPlrunaioningorlk~. lrsnyofthc can&, ampmu arh rc cllvirmnccltll motsminonta nff~Urhl.nacanpmvukeuecssiwpoduc(ion of ROS that is effaivcly wawgcd by ~~ antioxidant defapt apbn"). In 7, sewrnl an- (w paein) (mg mu) SuprmkCdim?Smb (ma padn) (mg DNA) Glu*hions-c (ma W) (ma DNA) aunhDncpm*duce (nlg ~m(cin) (w DNA) &or w -9 (ms Imuin) (mg mu) wpaojdsaon' (W &) 5.28tO.!a 5.61tO.M' (mo DNA) 4.Pr0.37 5.74ro.39' PC0.05 W m a d u a m liOxi&"l *lg P6 glutahhe pauxidasI"', supmai* ciismu~py[al catadm) u. kmwn to w. Cytqbn of spmnmm is uuanly liud in volume In mnclusicm. fhe pent studies mflcct that linand WWi, a the polpmauwd fmty acids that dune indm oliiduiw smas in rat epididym* Md epibound in Ihe spurn p l w mmbnrr are wy sumbk lo RQS d~Y'. els or an~ioxidmt sraym~l with a pdbie raductiMl in e- didw spm, by inacasing ROS snd dsming levkubxidut auym rmrrliurce a mutually support- pididymal sprm mnD Md qididyd sperm molility. in ICM of defence against ROS. In Ua pocnt sudy thc rpoific rtivitic. of atnlar, rupaoxidc dimtase, #lulnthnrr tuk@nsE onl gluwi pcrmk WM lurd lo hc k& whik nn inumff in thc hydmgen m ilulhas h1k UT SW~T of Bioinfmtics CenpaoxWcpaxmcionMdlipid~wuc~ UP., Pmlichcrry <strong>University</strong>. P~dichury lw pviding in~Md@did~rpnnoflklindPno~- -a. fdtia. KCC vluawicdges My Tata MMOed n(r. Tlnm in Ua pwuu thc incrasc in lipid rhl Twt. W, India fw the Junior Sehol~6hip. -Mi with a rrduction in .n(iori atym in- a Pcxmwkdgu Mian Cauril of Medical Ra+arrh,
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FUNCTIONAL STUDIES ON THE EFFECTS O
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ACKNOWLEDGEMENTS I wish to express
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TABLE OF CONTENTS Page No ACKNOWLED
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TABLE OF CONTENTS (Continued) Deter
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TABLE OF CONTENTS (Continued) Effec
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TAULIC OF CON'I'ISN'I'S (C'untinued
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xi LIST OF TABLES (Continued) I'agc
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Table 24a Table 24b Table 24c Table
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Fig. 1 Fig. 2 Effect of TCDD on the
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Fig. 12c Fig. 12d Fig. 13 Fig. 14a
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LIST OF FIGURES (Continued) Fig. 23
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LH M PL mg mRNA N AD NADH NADP NADP
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layers, an outer layer of visceral
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tubular epithelium and continually
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lmn~unization of monkeys against FS
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and this reaction is catalyzed by t
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extratubular environment or synthes
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the most active in protein secretio
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1958). A rapid fall in serum testos
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have also been reported in the rats
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Effect of TCDD on heart muscle has
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1.4 Effect of oxidative stress on m
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2 SCOPE OF THE PRESENT STUDY There
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3 MATERIALS AND METHODS 3.1 Animals
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Subgroup III: Administration of TCD
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choppcd with the help of sharp razo
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anti-FSH antibody. After incubation
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lust sped (500 to 700 rpm) on an or
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3.13.4 Quantitative determinntiun u
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The plates were dried by inverting
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centrifuged at 3,500 g for I5 min o
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3.17 Preparation of tiasue humogena
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supcrnutant was taken. Hlunk was pr
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3.21.4 Assay of glutathione peroxid
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4 RESULTS 4.1 Administration of TCD
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seminiferous tubules was found to b
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fractions of rat testis as compared
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4.1.11.1 Effect of TCDD on the prod
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4.2 Co-administration of TCDD and v
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4.2.8 Effect of co-administration o
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4.2.11.5 Effect of co-administratio
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Table I b. Effect of TCDD on the we
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Table 4. Effect of TCDD on the seru
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Table 7b. Effect of TCDD on the pro
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Table 8c. Effect of TCDD on the lev
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Table 9d. Effect of TCDD on the act
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Table 13. Effect of TCDD on the ant
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Table 15. Effect of TCDD on the lev
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Table 16c. Effcct of TCDD on the an
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Table 18a. Effect of co-administrat
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Table 21. Effect of co-administrati
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Table 24b. Effect of co-administrat
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Table 25c. Effect of co-administrat
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Table 26d. Effect of co-administrat
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Table 30. Effect of co-administrati
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Table 32. Effect of caadrninistrati
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Table 33c. Effcct of co-administrut
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5 DISCUSSION 5.1 Significance of th
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Measurement of weights of the acces
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compartment of the cells (Chainy el
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the reduction in daily sperm produc
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eported in the TCDD-treated rats (M
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lilnction is to ililcrccpt lipid pc
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in the animals administered with TC
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protective effects on serum testost
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suygcstccl that vivamin 1: conld im
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Fig.4~. ENect of TCDD or TCDD and v
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tE W d In w it I I (,OCX) 3s PUB (,
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Fig. 7c. Effect of TCDD or TCDD and
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u 3 f ~ = m w * N O .- ~Slew aew en
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Fig.lOb. ENmt of TCDD or TCDD and v
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Fig. 1Ir Effect of TCDD or TCDD md
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Fig. 14c. Effect of TCDD or TCDD an
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Fig. 16b. Effect of TCDD or TCDD an
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Fig.18~. EKect of TCDD or TCDD and
Page 166 and 167: 1 1.2 l4 Fig. 19b. ENect of TCDD or
Page 168 and 169: Fig. ZOb. Elkt of TCDD or TCDD and
Page 171 and 172: Fig. 21. Effect olTCDD or TCDD and
Page 173 and 174: Fig. 22c. Effect of TCDD or TCDD an
Page 175 and 176: Fig. 24b. Eflect ofTCDD or TCDD and
Page 177 and 178: 7 REFERENCES Aitken RJ, Irvine DS,
Page 179 and 180: Chitm KC, Latchoumycandanc,C, Mathu
Page 181 and 182: Flsher JS, Turner KJ, Brown D, Shar
Page 183 and 184: Hornsby PJ. Regulation of cytochrom
Page 185 and 186: Latcboumycandane C, Chitra KC, Math
Page 187 and 188: Moore RW, Panona JA, Bookstaff RC,
Page 189 and 190: Rommerts FFG, Cooke BA, Van Der Kem
Page 191 and 192: Slaunwhite WR, Burgett MJ. h virro
Page 193 and 194: 1.1 Preparation of dosing solution
Page 195 and 196: 1.4.6 Wash concentrate One bottle,
Page 197 and 198: 1.7.3 Estradiol controls Two vials.
Page 199 and 200: 1.1 1.2 Orcinol(6%) 6 g of orcinol
Page 201 and 202: 1.16.2 Horseradish peroxidase (8 Un
Page 203 and 204: 1.20 Superoxide dismutase 1.20.1 Tr
Page 205 and 206: 1.24.2 Thiobarbituric acid (TBA; 0.
Page 207 and 208: Chronic effect: of enclosulfan on t
Page 209: enbw1h1.W mu IUY Wcab unpiliml~t at
Page 212 and 213: cbniuis 11~11 b"nci:~lc ~wlive oxyg
Page 214 and 215: DNA, RNA ;uxl Wcin wcrr abarval, wh
Page 218 and 219: New Delhi, llrliil Ib SCI~KK Rcm& M
Page 220 and 221: tiii~lc rcprmluction or rut (Cllitr
Page 222 and 223: wciglit (b.wt.) per day for 45 days
Page 224 and 225: 0.1 00 01 " 0.8 I . .lo Dl* rcnn IW
Page 226 and 227: Chilr:~. K.C.. L!olnn!myci!~dum. C.
Page 228 and 229: C. hteboumyeradlos . KC. CMtn . P.P
Page 230 and 231: I. 1 Ilal 01 2 1.7 # I c I ~ ~ L I
Page 232 and 233: Fin. L Corrclatwn bclwfcn "perm cou
Page 235: Iionshii nepmdvctivc mxicity ad upt
Page 240 and 241: o. Glutathione reductage mg pmWn mg
Page 242 and 243: IOWAME -. Tor. #558517 PME: 9 SESS:
Page 244 and 245: JOBNAME: Rqm. Tm. M5W7 PAOB: 11 SBS
Page 246 and 247: t 02 ELSEVIER Toricology OM) (2W1)
Page 248 and 249: ---- - wcre obtu~ncd from E-Monk. G
Page 250: 3.2. A,III~AILNI syrlo~t of ,nIroch
Page 253 and 254: Uc,#!dy. SC. N;tdcru. S , 1% Conlrb
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ON TESTIS AND EPlDlDYMlS OF RATS - Pondicherry University ...

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