ON TESTIS AND EPlDlDYMlS OF RATS - Pondicherry University ...

obtained from Sisco Research Laboratories Pvt. Ltd.. Mumbai, India. Glutathione
reduced and glutathione oxidized were obtained from Sd. fine Chem Ltd. Mumbai.
All other chemicals were of analytical grade and obtained from local commercial
sources.
3.4 Handling of TCDD and animals
All materials contaminated with TCDD were treated as hazardous waste.
Gloves, goggles. mask and laboratory coats were used while handling the powdered
material and subsequent solutions. The animals were maintained and handled
according to the guidelines from the Indian Council of Medical Research, New Delhi.
3.5 Treatments
The experiments were carried out in pubertal (postnatal days 45) male rats. In
each experiments, corresponding groups of control animals, treated with vehicle alone
were maintained and killed along with the treated rats. The treatments to various
groups of rats are described below.
3.5.1 Group I: Admhlatration of TCDD to pubertal rats
TCDD was dissolved in acetone and olive oil (see Appendix 1.1) in the ratio
of 1 :I9 and administered orally for 45 days. For the administration of TCDD, the
pipette tip was gently placed just inside the mouth and the dosing solution was then
carefully expelled from the tip allowing the animal to lick the compound from the tip.
Corresponding groups of animals were administered with equal volume of vehicle
alone and served as control.
Subgroup I : Administration of TCDD at the dose of 1 nd Kg body weight/
day.
Subgroup I1 : Adminiseation of TCDD at the dose of 10 ng/ Kg body weight/
by.