ON TESTIS AND EPlDlDYMlS OF RATS - Pondicherry University ...

1.22.2 NADPH (0.2 pM)
16.6672 mg of NADPH was dissolved in 10 mL of distilled water
1.22.3 Glutathione oxidized (2 mM)
Glutathione oxidized of 12.2526 my was dissolved in 10 mL of distilled water
(2 mM).
1.22.4 Ethylenediaminetetraacetic acid (EDTA, 0.01 M)
37.224 mg of EDTA was dissolved in I0 mL of distilled water.
1.23 Glutathione peroxidase
1.23.1 Phosphate buffer (0.05 M. pH 7.0)
0.89 g of disodium hydrogen phosphate was dissolved in 100 mL of distilled
water (Solution A).
0.78005 g of sodium dihydrogen phosphate was dissolved in 100 mL of
distilled water (Solution B).
Take 39 mL of solution A and 61 ml of solution B was taken and diluted to
200 ml and pH was adjusted to 7.6
1.23.2 Glutathione reductase
3 1.25 mg of glutathione reductase was dissolved in 5 mL of distilled water.
1.23.3 EDTA (0.01 M)
37.224 mg of EDTA was dissolved in 10 mL of distilled water.
1.23.4 Sodium azide
6.5 rng of sodium azide was dissolved in 10 mL of distilled water.
1.23.5 Glutathione (reduced)
30.733 mg of glutathione (reduced) was dissolved in 10 mL of distilled water.
1.23.6 NADPH (0.2 pM)
16.6672 mg of NADPH was dissolved in I0 mL of distilled water.
1.24 Lipid peroxidation
1.24.1 15% v/ v trichloro acetic acid (TCA)
15 mL of 100 % TCA was made up to 100 mL with distilled water.