ON TESTIS AND EPlDlDYMlS OF RATS - Pondicherry University ...

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Filk Effect of TCDD or TCDD and vitamin E on the hydrogen peroxide generation in crude homogenatc, mitocbondrhl m d microsome-rich fnctiow of rat tatis (munfSD; *P
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FUNCTIONAL STUDIES ON THE EFFECTS O
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ACKNOWLEDGEMENTS I wish to express
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TABLE OF CONTENTS Page No ACKNOWLED
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TABLE OF CONTENTS (Continued) Deter
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TABLE OF CONTENTS (Continued) Effec
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TAULIC OF CON'I'ISN'I'S (C'untinued
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xi LIST OF TABLES (Continued) I'agc
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Table 24a Table 24b Table 24c Table
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Fig. 1 Fig. 2 Effect of TCDD on the
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Fig. 12c Fig. 12d Fig. 13 Fig. 14a
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LIST OF FIGURES (Continued) Fig. 23
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LH M PL mg mRNA N AD NADH NADP NADP
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layers, an outer layer of visceral
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tubular epithelium and continually
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lmn~unization of monkeys against FS
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and this reaction is catalyzed by t
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extratubular environment or synthes
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the most active in protein secretio
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1958). A rapid fall in serum testos
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have also been reported in the rats
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Effect of TCDD on heart muscle has
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1.4 Effect of oxidative stress on m
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2 SCOPE OF THE PRESENT STUDY There
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3 MATERIALS AND METHODS 3.1 Animals
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Subgroup III: Administration of TCD
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choppcd with the help of sharp razo
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anti-FSH antibody. After incubation
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lust sped (500 to 700 rpm) on an or
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3.13.4 Quantitative determinntiun u
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The plates were dried by inverting
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centrifuged at 3,500 g for I5 min o
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3.17 Preparation of tiasue humogena
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supcrnutant was taken. Hlunk was pr
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3.21.4 Assay of glutathione peroxid
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4 RESULTS 4.1 Administration of TCD
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seminiferous tubules was found to b
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fractions of rat testis as compared
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4.1.11.1 Effect of TCDD on the prod
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4.2 Co-administration of TCDD and v
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4.2.8 Effect of co-administration o
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4.2.11.5 Effect of co-administratio
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Table I b. Effect of TCDD on the we
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Table 4. Effect of TCDD on the seru
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Table 7b. Effect of TCDD on the pro
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Table 8c. Effect of TCDD on the lev
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Table 9d. Effect of TCDD on the act
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Table 13. Effect of TCDD on the ant
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Table 15. Effect of TCDD on the lev
Page 98 and 99: Table 16c. Effcct of TCDD on the an
Page 100 and 101: Table 18a. Effect of co-administrat
Page 103 and 104: Table 21. Effect of co-administrati
Page 105 and 106: Table 24b. Effect of co-administrat
Page 107 and 108: Table 25c. Effect of co-administrat
Page 109 and 110: Table 26d. Effect of co-administrat
Page 111 and 112: Table 30. Effect of co-administrati
Page 113 and 114: Table 32. Effect of caadrninistrati
Page 115 and 116: Table 33c. Effcct of co-administrut
Page 117 and 118: 5 DISCUSSION 5.1 Significance of th
Page 119 and 120: Measurement of weights of the acces
Page 121 and 122: compartment of the cells (Chainy el
Page 123 and 124: the reduction in daily sperm produc
Page 125 and 126: eported in the TCDD-treated rats (M
Page 127 and 128: lilnction is to ililcrccpt lipid pc
Page 129 and 130: in the animals administered with TC
Page 131 and 132: protective effects on serum testost
Page 133: suygcstccl that vivamin 1: conld im
Page 136 and 137: Fig.4~. ENect of TCDD or TCDD and v
Page 138: tE W d In w it I I (,OCX) 3s PUB (,
Page 142 and 143: Fig. 7c. Effect of TCDD or TCDD and
Page 145 and 146: u 3 f ~ = m w * N O .- ~Slew aew en
Page 147: Fig.lOb. ENmt of TCDD or TCDD and v
Page 157 and 158: Fig. 14a. Effect of TCDD or TCDD an
Page 159 and 160: Fig. 1Sb. Effect of TCDD or TCDD an
Page 161: Fig.16d. Effect of TCDD or TCDD and
Page 165 and 166: Fig. 19a. Effect of TCDD or TCDD an
Page 167 and 168: 60 Fig. 20a. Effect of TCDD or TCDD
Page 169: Fig. 2k. Ekt of TCDD or TCDD and vi
Page 172 and 173: Fig. 22a. EKect of TCDD or TCDD and
Page 174 and 175: Fig. 23b. Effect of TCDD or TCDD an
Page 176 and 177: Fig. 24d. Effect of TCDD or TCDD an
Page 178 and 179: Burkhart ER. A study of the early e
Page 180 and 181: de Kretaer DM. In: The Testis. Burg
Page 182 and 183: Griawold MD. Protein secretion by S
Page 184 and 185: June NHPM, Jansen R, Grootegoed JA,
Page 186 and 187: Mathur PP, Mo MY, Panzironi C, Silv
Page 188 and 189: Petenon RE, Scefeld MD, Christian W
Page 190 and 191: Shan LX. Hardy MP. Developmental ch
Page 192 and 193: Tindall DJ, MiUer DA, Means AR. Cha
Page 194 and 195: 1.3.4 Antibody-Enzyme Conjugate sol
Page 196 and 197: 1.6.2 Testosterone standards One vi
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22.91 pg of 4-androstcne 3,17-dione
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1.13 Subcellulnr frnctionation of t
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1.17.4 Disodium hydrogen phosphate
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1.22.2 NADPH (0.2 pM) 16.6672 mg of
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APPENDIX 2 LIST OF PUBLICATIONS OF
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~lLllc mu ~LYIC~L~ II*: ~[IL'IIII W
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Effect of lindane on testicular ant
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ILXlll hnl, .I 121; 1.0 -/.a . Ill
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Effect of lindane on antioxidant en
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WnCr, inyminn~nt of spmri motility,
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The effect of 2,3,7,8-tetrachlorodi
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I kl
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C'. I~~I~~~~IIIII~~O~,~~~~~~ R6+ 4,
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'lstrr,tb~xr 171 i?lYJ?I 127 /.IS I
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S.tlc. S.. ?IXII. Mubuli~r bioluyy
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(I't,l:~t~cl 211x1 Kin~hronglt IVX4
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t11.3. Wal 01 TCDD on !he acttnly o
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IIR h r k a ~ r Ilw ~ ;njllmr~ l ~
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C L m r ~ n . l l ~ T a d ~ 1 6 W J
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c urbmwen&n= s a( I n.pradpradpradn
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- aaarslu30 ,.*mra. L~jl;lr; -P4-w~
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(=I 23. w- C Olln (LC W W. Ubrth d-
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continuous exposure of humans to in
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pH 7.6). I00 pl of 200 mM NADPH, IW
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ioavaiiability andlor production of
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Glutnchlone irrox$dssc, rupcioridc
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