ON TESTIS AND EPlDlDYMlS OF RATS - Pondicherry University ...

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IOWAME -. Tor. #558517 PME: 9 SESS: 5 OUTPUT: Tuc Jan 29 10:00:51 2002 hieblW-mM1-#mOlm/msr26-02. - 1- in th opididYIni1 Md a dcuura in the weight of thaxychlor into Its mujar melaboiitrr and CYPlA2 hrr Ihc 80% Orom8 my dn lea biwv&ility bccn shown lo play a predominant role bn rhlr reaction Or pmdvaiw Of Mdrapsn in wtbozychlor--mi rau. IIZI. The ability of the cytochrome P450 syslcm to in- Tho weigh1 of tho Uvu UKI Udvy dld not ihow any duce the pmdvction of ROS has ken wcll documented lloniacMI in tho MbmIr lmald~wid Moxy. 1131. In the present study, admnisuatian of methaxychla. OnY Q II. npottcd IhQ ldmiairrruion of mc- chlor dccrurrcd the activrtics of catslare, superoxids dis- IhOxyshlor Q dce4 &@ng from 200 c 400 mglkdday murase. plutath~onc reduclass, and giutslh~one pcroxifar 11 nwr& dlarod tho W y wci@ htd veiphtr of the darc, and concom3untly increased the Levels of hydrogen live# Md Udmy 1361. Thc ebomr & of admininn- perondc and lipid . . proridstion in eddidymnl rwrm 85 tion in out uwiy-nuy -I fcxiho I.ek of o b ~ ~ e d wcll as in the cpididymis. Suprox~dc dismutasc ia cunetion on (b..o a* wciphu. Thc acb.niim of momol;ychla-mediated oxidative rtdered the fimt line of defense against deleterious cffccrr of oxyradicsll in Ihe cell by coulynng the d~rmuiot$on ~irfflc~butilhu~lbowncbomsdiaudby superoxide radicals to hydrogen peromde und molecuivr Ihc vdvuloll of nkxumnl moaoorygenue, which is involved in tho WII~!QXI of mDZboxysbior iaw iu re. vdn mmbliccr 1101. Dudly Uhir nrtioa. mtivc oxygen. The reduction in thc aclivity of catalase m;ly reflect an inability of cpididymal sperm md the epidid. ymir lo eliminate hydropcn pmxide prcduced by the M $ V & poulbly fno ndiul:, bind covllently to activation of methoxychlor and iu mclabolilcr or lo enmi- mmp~wmr 1101. ~stioxid.n~fioe r.dic~.i zyme mscuvauon caused by excess ROS production In luvenlar lad hirulfhydiyisonuini~ compounds Inhibit COV&lll Mlldiq of maborycNa in hwnrn llver microcpsdidymai sperm and the cpididymls 1371 The nnrioxldant enzymes caalase and peroiidaic prolocl ruperoude unna, ruwdng Uul UT M~VC inlediate is I. flK dirmutssc agatnst ~nactlvation by hydrogen proxidc. Rendtul 1101. It hu been rhown that human cylahmme P450 my- po rolporulblc of rhc converrion of mcctpmcally. 1hC rvperoxide d~rmulasc protects cntalasc and prnxidase against inhrbitian by superoxide anlon.
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FUNCTIONAL STUDIES ON THE EFFECTS O
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ACKNOWLEDGEMENTS I wish to express
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TABLE OF CONTENTS Page No ACKNOWLED
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TABLE OF CONTENTS (Continued) Deter
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TABLE OF CONTENTS (Continued) Effec
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TAULIC OF CON'I'ISN'I'S (C'untinued
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xi LIST OF TABLES (Continued) I'agc
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Table 24a Table 24b Table 24c Table
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Fig. 1 Fig. 2 Effect of TCDD on the
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Fig. 12c Fig. 12d Fig. 13 Fig. 14a
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LIST OF FIGURES (Continued) Fig. 23
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LH M PL mg mRNA N AD NADH NADP NADP
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layers, an outer layer of visceral
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tubular epithelium and continually
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lmn~unization of monkeys against FS
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and this reaction is catalyzed by t
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extratubular environment or synthes
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the most active in protein secretio
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1958). A rapid fall in serum testos
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have also been reported in the rats
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Effect of TCDD on heart muscle has
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1.4 Effect of oxidative stress on m
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2 SCOPE OF THE PRESENT STUDY There
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3 MATERIALS AND METHODS 3.1 Animals
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Subgroup III: Administration of TCD
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choppcd with the help of sharp razo
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anti-FSH antibody. After incubation
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lust sped (500 to 700 rpm) on an or
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3.13.4 Quantitative determinntiun u
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The plates were dried by inverting
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centrifuged at 3,500 g for I5 min o
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3.17 Preparation of tiasue humogena
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supcrnutant was taken. Hlunk was pr
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3.21.4 Assay of glutathione peroxid
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4 RESULTS 4.1 Administration of TCD
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seminiferous tubules was found to b
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fractions of rat testis as compared
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4.1.11.1 Effect of TCDD on the prod
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4.2 Co-administration of TCDD and v
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4.2.8 Effect of co-administration o
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4.2.11.5 Effect of co-administratio
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Table I b. Effect of TCDD on the we
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Table 4. Effect of TCDD on the seru
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Table 7b. Effect of TCDD on the pro
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Table 8c. Effect of TCDD on the lev
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Table 9d. Effect of TCDD on the act
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Table 13. Effect of TCDD on the ant
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Table 15. Effect of TCDD on the lev
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Table 16c. Effcct of TCDD on the an
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Table 18a. Effect of co-administrat
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Table 21. Effect of co-administrati
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Table 24b. Effect of co-administrat
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Table 25c. Effect of co-administrat
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Table 26d. Effect of co-administrat
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Table 30. Effect of co-administrati
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Table 32. Effect of caadrninistrati
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Table 33c. Effcct of co-administrut
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5 DISCUSSION 5.1 Significance of th
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Measurement of weights of the acces
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compartment of the cells (Chainy el
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the reduction in daily sperm produc
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eported in the TCDD-treated rats (M
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lilnction is to ililcrccpt lipid pc
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in the animals administered with TC
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protective effects on serum testost
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suygcstccl that vivamin 1: conld im
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Fig.4~. ENect of TCDD or TCDD and v
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tE W d In w it I I (,OCX) 3s PUB (,
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Fig. 7c. Effect of TCDD or TCDD and
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u 3 f ~ = m w * N O .- ~Slew aew en
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Fig.lOb. ENmt of TCDD or TCDD and v
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Fig. 1Ir Effect of TCDD or TCDD md
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Fig. 14c. Effect of TCDD or TCDD an
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Fig. 16b. Effect of TCDD or TCDD an
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Fig.18~. EKect of TCDD or TCDD and
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1 1.2 l4 Fig. 19b. ENect of TCDD or
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Fig. ZOb. Elkt of TCDD or TCDD and
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Fig. 21. Effect olTCDD or TCDD and
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Fig. 22c. Effect of TCDD or TCDD an
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Fig. 24b. Eflect ofTCDD or TCDD and
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7 REFERENCES Aitken RJ, Irvine DS,
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Chitm KC, Latchoumycandanc,C, Mathu
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Flsher JS, Turner KJ, Brown D, Shar
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Hornsby PJ. Regulation of cytochrom
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Latcboumycandane C, Chitra KC, Math
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Moore RW, Panona JA, Bookstaff RC,
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Rommerts FFG, Cooke BA, Van Der Kem
Page 191 and 192: Slaunwhite WR, Burgett MJ. h virro
Page 193 and 194: 1.1 Preparation of dosing solution
Page 195 and 196: 1.4.6 Wash concentrate One bottle,
Page 197 and 198: 1.7.3 Estradiol controls Two vials.
Page 199 and 200: 1.1 1.2 Orcinol(6%) 6 g of orcinol
Page 201 and 202: 1.16.2 Horseradish peroxidase (8 Un
Page 203 and 204: 1.20 Superoxide dismutase 1.20.1 Tr
Page 205 and 206: 1.24.2 Thiobarbituric acid (TBA; 0.
Page 207 and 208: Chronic effect: of enclosulfan on t
Page 209: enbw1h1.W mu IUY Wcab unpiliml~t at
Page 212 and 213: cbniuis 11~11 b"nci:~lc ~wlive oxyg
Page 214 and 215: DNA, RNA ;uxl Wcin wcrr abarval, wh
Page 216 and 217: Mt~~m(10-lZsu&)vacob- Wfmn Ihehml ~
Page 218 and 219: New Delhi, llrliil Ib SCI~KK Rcm& M
Page 220 and 221: tiii~lc rcprmluction or rut (Cllitr
Page 222 and 223: wciglit (b.wt.) per day for 45 days
Page 224 and 225: 0.1 00 01 " 0.8 I . .lo Dl* rcnn IW
Page 226 and 227: Chilr:~. K.C.. L!olnn!myci!~dum. C.
Page 228 and 229: C. hteboumyeradlos . KC. CMtn . P.P
Page 230 and 231: I. 1 Ilal 01 2 1.7 # I c I ~ ~ L I
Page 232 and 233: Fin. L Corrclatwn bclwfcn "perm cou
Page 235: Iionshii nepmdvctivc mxicity ad upt
Page 240 and 241: o. Glutathione reductage mg pmWn mg
Page 244 and 245: JOBNAME: Rqm. Tm. M5W7 PAOB: 11 SBS
Page 246 and 247: t 02 ELSEVIER Toricology OM) (2W1)
Page 248 and 249: ---- - wcre obtu~ncd from E-Monk. G
Page 250: 3.2. A,III~AILNI syrlo~t of ,nIroch
Page 253 and 254: Uc,#!dy. SC. N;tdcru. S , 1% Conlrb
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