ON TESTIS AND EPlDlDYMlS OF RATS - Pondicherry University ...

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Tindall DJ, MiUer DA, Means AR. Characterization of androgen receptor in Sertoli cell enriched testis. Endocrinology 1977; 101 : 13-23. Tramer F, Rocco F, Micalli G, Sandri G, Panfili E. Antioxidant systems in rat epididyrnal spermatozoa. Biol Reprod 1998; 59: 735-758. Turner JN, Collins DN. Liver morphology in guinea pigs administcred eithcr pyrolysis products of a polychlorinated biphenyls transformer fluid or 2.3,7,8- tctrachlorodibenzo-p-dloxin. Toxicol Appl Pharmacol. 1983; 67: 417429. Turner TT, Jonar CE, Howards SS, Ewing LL, w eye B, Gunsalus GL. On the androgen microenvironment of maturing spermatozoa. Endocrinology 1984; 1 IS: 1925-1932. US Environmental Protection Agency. OPPTS Hormonited test guidelines. 870.3800. Reproduction and fertility effects. USA EPA (1998), Washington DC. Verhoeven G. Androgen receptor in cultured interstitial cells derived from immature rat testis. J Steroid Biochem 1980; 13: 469-474. Verhoeven G, Cailleau J. Specificity and partial purification of a-factor in spent media from Sertoli cell-enriched culttues that stimulates steroidogenesis in Leydig cells. J Steroid Biochem 1986; 25: 393-402. Weinbauer GF, Gockeler E, Nieschlag E. Testosterone prevents complete suppression of spermatogenesis in the gonadotrophin releasing hormone (GnRH) antagonistic-treated nonhuman ~rirnate (Mococu f~tcicularis). J Clin Endocrinol Weinbauer GF, Behre HM, Fingscheidt U, Nieschlag E. Human follicle stimulating hormone exerts a stimulatory effect on spermatogenesis. testicular size, and senun inhibin levels in the gonadotrophin releasing hormone antagonisttreated nonhuman primate (Mucucu fuscicukuri.~). Endocrinology 1991 ; 129: 1831-1839. WHO Laboratory Manual. (1999). - for the examination of human semen and sperm-cervical mucus interaction. Cambridge University Press. Wilker CE. Disruption of the development and function of the male reproductive system by 2.3.7.8-tetrachlorodibem-p-dioxin and indole-3- carbinol. Diss Abst Int Sci 1996; 57: 3694B. Wing TY. Christensen AK. Morphometric studies on rat seminiferous tubules. Am 3 Anat 1982; 165: 13-25. Worley RTS, Nicbobon HD, Pickering BT. Testicular oxytocin: an initiator of seminiferous tubule movement?. Coll MSERM 1984; 123 : 205-2 12. Zipf WE, Payne AH, Kelch RP. Prolactin. grorowth hormone, and luteinizing hormone in the maintenance of testicular luteinizing hormone receptors. Endocrinology 1978; 103: 595400.
1.1 Preparation of dosing solution APPENDIX 1 PREPARATION OF REAGENTS 1.1.1 2.3.7.8-Tetrachlorodibem-p-dioxin (TCDD) 100 pg of TCDD was dissolved in acetone and olive oil (1:19) and used as stock solution. The stock solution was serially diluted and used for treatments. 1.1.2 Vitamin E 200 mg of vitamin E was made up to 10 mL with olive oil and stored in amber coloured bottle at 4OC. This solution contained 20 mg vitamin E/ mL. 1.2 Epididymal sperm counts 1.2.1 Diluents The diluents for sperm counts were prepared by the addition of 50 g sodium bicarbonate, 10 mL 35% formalin and 0.25 g trypan blue in a volumetric flask and made up to a final volume of 1 L with distilled water. 1.3 Serum FSH 1.3.1 Anti-FSH-Coated microtitration strips One strip holder contained 96 microtitration wells coated with anti-FSH antibody. Stored at 2-8'C in the resealable pouch with a desiccant to protect from moisture. 1.3.2 FSH standards One vial, 2 mL, labeled A contained 0 mlU1 mL and five vials. 1 mL each, labeled B-F, contained concentrations of approximately 1.5, 4.5, 15, 45 and 150 mIU/ mL FSH (from human pituitary gland) in porcine serum with a nonmercury preservative. 1.3.3 FSH controls Two vials, I rnL each, Levels I and 11. contained low and high concentrations of FSH (from human pituitary gland) in porcine serum with a non-mercury preservative.
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FUNCTIONAL STUDIES ON THE EFFECTS O
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ACKNOWLEDGEMENTS I wish to express
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TABLE OF CONTENTS Page No ACKNOWLED
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TABLE OF CONTENTS (Continued) Deter
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TABLE OF CONTENTS (Continued) Effec
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TAULIC OF CON'I'ISN'I'S (C'untinued
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xi LIST OF TABLES (Continued) I'agc
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Table 24a Table 24b Table 24c Table
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Fig. 1 Fig. 2 Effect of TCDD on the
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Fig. 12c Fig. 12d Fig. 13 Fig. 14a
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LIST OF FIGURES (Continued) Fig. 23
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LH M PL mg mRNA N AD NADH NADP NADP
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layers, an outer layer of visceral
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tubular epithelium and continually
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lmn~unization of monkeys against FS
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and this reaction is catalyzed by t
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extratubular environment or synthes
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the most active in protein secretio
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1958). A rapid fall in serum testos
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have also been reported in the rats
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Effect of TCDD on heart muscle has
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1.4 Effect of oxidative stress on m
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2 SCOPE OF THE PRESENT STUDY There
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3 MATERIALS AND METHODS 3.1 Animals
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Subgroup III: Administration of TCD
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choppcd with the help of sharp razo
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anti-FSH antibody. After incubation
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lust sped (500 to 700 rpm) on an or
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3.13.4 Quantitative determinntiun u
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The plates were dried by inverting
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centrifuged at 3,500 g for I5 min o
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3.17 Preparation of tiasue humogena
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supcrnutant was taken. Hlunk was pr
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3.21.4 Assay of glutathione peroxid
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4 RESULTS 4.1 Administration of TCD
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seminiferous tubules was found to b
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fractions of rat testis as compared
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4.1.11.1 Effect of TCDD on the prod
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4.2 Co-administration of TCDD and v
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4.2.8 Effect of co-administration o
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4.2.11.5 Effect of co-administratio
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Table I b. Effect of TCDD on the we
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Table 4. Effect of TCDD on the seru
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Table 7b. Effect of TCDD on the pro
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Table 8c. Effect of TCDD on the lev
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Table 9d. Effect of TCDD on the act
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Table 13. Effect of TCDD on the ant
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Table 15. Effect of TCDD on the lev
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Table 16c. Effcct of TCDD on the an
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Table 18a. Effect of co-administrat
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Table 21. Effect of co-administrati
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Table 24b. Effect of co-administrat
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Table 25c. Effect of co-administrat
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Table 26d. Effect of co-administrat
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Table 30. Effect of co-administrati
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Table 32. Effect of caadrninistrati
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Table 33c. Effcct of co-administrut
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5 DISCUSSION 5.1 Significance of th
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Measurement of weights of the acces
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compartment of the cells (Chainy el
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the reduction in daily sperm produc
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eported in the TCDD-treated rats (M
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lilnction is to ililcrccpt lipid pc
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in the animals administered with TC
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protective effects on serum testost
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suygcstccl that vivamin 1: conld im
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Fig.4~. ENect of TCDD or TCDD and v
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tE W d In w it I I (,OCX) 3s PUB (,
Page 142 and 143: Fig. 7c. Effect of TCDD or TCDD and
Page 145 and 146: u 3 f ~ = m w * N O .- ~Slew aew en
Page 147 and 148: Fig.lOb. ENmt of TCDD or TCDD and v
Page 151: Fig. 1Ir Effect of TCDD or TCDD md
Page 158 and 159: Fig. 14c. Effect of TCDD or TCDD an
Page 160 and 161: Fig. 16b. Effect of TCDD or TCDD an
Page 164 and 165: Fig.18~. EKect of TCDD or TCDD and
Page 166 and 167: 1 1.2 l4 Fig. 19b. ENect of TCDD or
Page 168 and 169: Fig. ZOb. Elkt of TCDD or TCDD and
Page 171 and 172: Fig. 21. Effect olTCDD or TCDD and
Page 173 and 174: Fig. 22c. Effect of TCDD or TCDD an
Page 175 and 176: Fig. 24b. Eflect ofTCDD or TCDD and
Page 177 and 178: 7 REFERENCES Aitken RJ, Irvine DS,
Page 179 and 180: Chitm KC, Latchoumycandanc,C, Mathu
Page 181 and 182: Flsher JS, Turner KJ, Brown D, Shar
Page 183 and 184: Hornsby PJ. Regulation of cytochrom
Page 185 and 186: Latcboumycandane C, Chitra KC, Math
Page 187 and 188: Moore RW, Panona JA, Bookstaff RC,
Page 189 and 190: Rommerts FFG, Cooke BA, Van Der Kem
Page 191: Slaunwhite WR, Burgett MJ. h virro
Page 195 and 196: 1.4.6 Wash concentrate One bottle,
Page 197 and 198: 1.7.3 Estradiol controls Two vials.
Page 199 and 200: 1.1 1.2 Orcinol(6%) 6 g of orcinol
Page 201 and 202: 1.16.2 Horseradish peroxidase (8 Un
Page 203 and 204: 1.20 Superoxide dismutase 1.20.1 Tr
Page 205 and 206: 1.24.2 Thiobarbituric acid (TBA; 0.
Page 207 and 208: Chronic effect: of enclosulfan on t
Page 209: enbw1h1.W mu IUY Wcab unpiliml~t at
Page 212 and 213: cbniuis 11~11 b"nci:~lc ~wlive oxyg
Page 214 and 215: DNA, RNA ;uxl Wcin wcrr abarval, wh
Page 216 and 217: Mt~~m(10-lZsu&)vacob- Wfmn Ihehml ~
Page 218 and 219: New Delhi, llrliil Ib SCI~KK Rcm& M
Page 220 and 221: tiii~lc rcprmluction or rut (Cllitr
Page 222 and 223: wciglit (b.wt.) per day for 45 days
Page 224 and 225: 0.1 00 01 " 0.8 I . .lo Dl* rcnn IW
Page 226 and 227: Chilr:~. K.C.. L!olnn!myci!~dum. C.
Page 228 and 229: C. hteboumyeradlos . KC. CMtn . P.P
Page 230 and 231: I. 1 Ilal 01 2 1.7 # I c I ~ ~ L I
Page 232 and 233: Fin. L Corrclatwn bclwfcn "perm cou
Page 235: Iionshii nepmdvctivc mxicity ad upt
Page 240 and 241: o. Glutathione reductage mg pmWn mg
Page 242 and 243:
IOWAME -. Tor. #558517 PME: 9 SESS:
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JOBNAME: Rqm. Tm. M5W7 PAOB: 11 SBS
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t 02 ELSEVIER Toricology OM) (2W1)
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---- - wcre obtu~ncd from E-Monk. G
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3.2. A,III~AILNI syrlo~t of ,nIroch
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Uc,#!dy. SC. N;tdcru. S , 1% Conlrb
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