ON TESTIS AND EPlDlDYMlS OF RATS - Pondicherry University ...

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to Fig. 15b; Table 29 and Fig. 19a lo I'if I9b; 'l'ablc 32). Administralion of'1'CL)I) along with vitamin E did not show any significant change in the activities of superoxide dismutase, catalase, glutathione reductase and glutathione peroxidase (Fig 12a to IZd, Table 26a to 26d; Fig. 16a to Fig. 16d; Table 30 and Fig. 20a to Fig. 2Od; Table 33a to 33c) as well as the level of lipid peroxidation (Fig.13; Table 27; Fig. 17; Table 30; and Fig. 21; Table 33a to 33c) in the crude homogenate, mitochondria1 and microsome-rich fractions of testis. epididymal sperm and various regions of the epididymis of rats as compared to the corresponding groups of control animals. The results suggested that vitamin E could protect testis and epididymis against TCDDinduced reactive oxygen species mediated toxicity. 5.3.7 Effect of co-administration of TCDD and vitamin E on antioxidant system in kidney Administration of TCDD along with vitamin E did not cause any significant change In the production of superoxide anion. nitric oxide and hydrogen peroxide in the kidney of rats as was shown to decrease in the TCDD-treated rats at 100 ng dose level (Fig. 22a to 22c; Table 34a). The levels of glutathione and a-tocopherol in the kldney of rats treated with TCDD and vitamin E remained unchanged (Fig.23a to 23b; 'Table 34b). There were, however, no changes in the levels of antioxidants in TCDD alone treated groups at lower doses such as I and I0 ng levels. Co-administration of TCDD and vitamin E did not cause any significant change in the activities of superoxide dismutase, catalase, glutathione reductase and glutathione peroxidase (Fig. 24a to 24d; Table 34c) as well as the level of lipid peroxidation in the kidney of rats as compared to the corresponding groups of control animals (Fig. 25; Table 34c). However, no significant changes were observed in the animals administered with TCDD at 1 and 10 ng dose levels. Low dose of TCDD were nor able to induce oxidative stress in kidney of rat thereby suggesting that thc antioxidant mechanism is higher in kidney as well as the toxicity threshold is lower in testis and epididymis than in kidney in terms of oxidative stress. The results
suygcstccl that vivamin 1: conld imp;lrt p~.otcclivc cI'(Lc~s i~gi~itlst 'I'('I>Il lo 11o11- reproductive tissue as well. 5.4 Epilogue On the basis of the foregoing discussion it can be summarized that exposure to TCDD increases oxidative stress in rats as evidenced by increase in reactive oxygen species, lipid peroxidation and suppression of antioxidant system in testis. epididymis, sperm and kidney. The generation of oxidative stress is triggered by low doses of TCDD in the male reproductive system as compared to kidney where it is generated only at high doses. Thus male reproductive system appears to be more susceptible to the damage caused by environmental contaminants like TCDD. Co-administration of TCDD along with vitamin E imparts protective effects against TCDD-induced reactive oxygen species mediated toxicity in the reproductive and non-reproductive tissues. Thus vitamin E could be uscd as a protective measure against TCDD-induced toxicity.
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FUNCTIONAL STUDIES ON THE EFFECTS O
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ACKNOWLEDGEMENTS I wish to express
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TABLE OF CONTENTS Page No ACKNOWLED
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TABLE OF CONTENTS (Continued) Deter
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TABLE OF CONTENTS (Continued) Effec
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TAULIC OF CON'I'ISN'I'S (C'untinued
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xi LIST OF TABLES (Continued) I'agc
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Table 24a Table 24b Table 24c Table
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Fig. 1 Fig. 2 Effect of TCDD on the
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Fig. 12c Fig. 12d Fig. 13 Fig. 14a
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LIST OF FIGURES (Continued) Fig. 23
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LH M PL mg mRNA N AD NADH NADP NADP
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layers, an outer layer of visceral
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tubular epithelium and continually
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lmn~unization of monkeys against FS
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and this reaction is catalyzed by t
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extratubular environment or synthes
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the most active in protein secretio
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1958). A rapid fall in serum testos
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have also been reported in the rats
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Effect of TCDD on heart muscle has
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1.4 Effect of oxidative stress on m
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2 SCOPE OF THE PRESENT STUDY There
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3 MATERIALS AND METHODS 3.1 Animals
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Subgroup III: Administration of TCD
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choppcd with the help of sharp razo
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anti-FSH antibody. After incubation
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lust sped (500 to 700 rpm) on an or
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3.13.4 Quantitative determinntiun u
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The plates were dried by inverting
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centrifuged at 3,500 g for I5 min o
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3.17 Preparation of tiasue humogena
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supcrnutant was taken. Hlunk was pr
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3.21.4 Assay of glutathione peroxid
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4 RESULTS 4.1 Administration of TCD
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seminiferous tubules was found to b
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fractions of rat testis as compared
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4.1.11.1 Effect of TCDD on the prod
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4.2 Co-administration of TCDD and v
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4.2.8 Effect of co-administration o
Page 82 and 83: 4.2.11.5 Effect of co-administratio
Page 84 and 85: Table I b. Effect of TCDD on the we
Page 86 and 87: Table 4. Effect of TCDD on the seru
Page 88 and 89: Table 7b. Effect of TCDD on the pro
Page 90 and 91: Table 8c. Effect of TCDD on the lev
Page 92 and 93: Table 9d. Effect of TCDD on the act
Page 94 and 95: Table 13. Effect of TCDD on the ant
Page 96 and 97: Table 15. Effect of TCDD on the lev
Page 98 and 99: Table 16c. Effcct of TCDD on the an
Page 100 and 101: Table 18a. Effect of co-administrat
Page 103 and 104: Table 21. Effect of co-administrati
Page 105 and 106: Table 24b. Effect of co-administrat
Page 107 and 108: Table 25c. Effect of co-administrat
Page 109 and 110: Table 26d. Effect of co-administrat
Page 111 and 112: Table 30. Effect of co-administrati
Page 113 and 114: Table 32. Effect of caadrninistrati
Page 115 and 116: Table 33c. Effcct of co-administrut
Page 117 and 118: 5 DISCUSSION 5.1 Significance of th
Page 119 and 120: Measurement of weights of the acces
Page 121 and 122: compartment of the cells (Chainy el
Page 123 and 124: the reduction in daily sperm produc
Page 125 and 126: eported in the TCDD-treated rats (M
Page 127 and 128: lilnction is to ililcrccpt lipid pc
Page 129 and 130: in the animals administered with TC
Page 131: protective effects on serum testost
Page 136 and 137: Fig.4~. ENect of TCDD or TCDD and v
Page 138: tE W d In w it I I (,OCX) 3s PUB (,
Page 142 and 143: Fig. 7c. Effect of TCDD or TCDD and
Page 145 and 146: u 3 f ~ = m w * N O .- ~Slew aew en
Page 147 and 148: Fig.lOb. ENmt of TCDD or TCDD and v
Page 151: Fig. 1Ir Effect of TCDD or TCDD md
Page 158 and 159: Fig. 14c. Effect of TCDD or TCDD an
Page 160 and 161: Fig. 16b. Effect of TCDD or TCDD an
Page 164 and 165: Fig.18~. EKect of TCDD or TCDD and
Page 166 and 167: 1 1.2 l4 Fig. 19b. ENect of TCDD or
Page 168 and 169: Fig. ZOb. Elkt of TCDD or TCDD and
Page 171 and 172: Fig. 21. Effect olTCDD or TCDD and
Page 173 and 174: Fig. 22c. Effect of TCDD or TCDD an
Page 175 and 176: Fig. 24b. Eflect ofTCDD or TCDD and
Page 177 and 178: 7 REFERENCES Aitken RJ, Irvine DS,
Page 179 and 180: Chitm KC, Latchoumycandanc,C, Mathu
Page 181 and 182: Flsher JS, Turner KJ, Brown D, Shar
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Hornsby PJ. Regulation of cytochrom
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Latcboumycandane C, Chitra KC, Math
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Moore RW, Panona JA, Bookstaff RC,
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Rommerts FFG, Cooke BA, Van Der Kem
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Slaunwhite WR, Burgett MJ. h virro
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1.1 Preparation of dosing solution
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1.4.6 Wash concentrate One bottle,
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1.7.3 Estradiol controls Two vials.
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1.1 1.2 Orcinol(6%) 6 g of orcinol
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1.16.2 Horseradish peroxidase (8 Un
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1.20 Superoxide dismutase 1.20.1 Tr
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1.24.2 Thiobarbituric acid (TBA; 0.
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Chronic effect: of enclosulfan on t
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enbw1h1.W mu IUY Wcab unpiliml~t at
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cbniuis 11~11 b"nci:~lc ~wlive oxyg
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DNA, RNA ;uxl Wcin wcrr abarval, wh
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Mt~~m(10-lZsu&)vacob- Wfmn Ihehml ~
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New Delhi, llrliil Ib SCI~KK Rcm& M
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tiii~lc rcprmluction or rut (Cllitr
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wciglit (b.wt.) per day for 45 days
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0.1 00 01 " 0.8 I . .lo Dl* rcnn IW
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Chilr:~. K.C.. L!olnn!myci!~dum. C.
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C. hteboumyeradlos . KC. CMtn . P.P
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I. 1 Ilal 01 2 1.7 # I c I ~ ~ L I
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Fin. L Corrclatwn bclwfcn "perm cou
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Iionshii nepmdvctivc mxicity ad upt
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o. Glutathione reductage mg pmWn mg
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IOWAME -. Tor. #558517 PME: 9 SESS:
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JOBNAME: Rqm. Tm. M5W7 PAOB: 11 SBS
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t 02 ELSEVIER Toricology OM) (2W1)
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---- - wcre obtu~ncd from E-Monk. G
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3.2. A,III~AILNI syrlo~t of ,nIroch
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Uc,#!dy. SC. N;tdcru. S , 1% Conlrb
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