ON TESTIS AND EPlDlDYMlS OF RATS - Pondicherry University ...

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cbniuis 11~11 b"nci:~lc ~wlive oxygen 01 5 .(I mvkg buJy wdght/JPy ra 3) drys. The control IROS)(~~. animals &ved a dmilor volw or lk whiele h. ROS nto M impumnt p;m or hu & r e m- ~wcnty-r~ hours slkr ~hc lat -I ~hc nisn iyninq inrmum. bt excnive gwwlion ol rm u+hd and killed ming mahdc akr. T*, opi. Oxygm dials #my ILunogwfc lisw. ROS arc fwmal in dkJymis, anid rrsickx and vcmd wee n. b h phys'rdogiul d pahlogicol mndilhs in mm- mod. c!mdofIhc.dhaingriaus.nlusi@. A mlim ti-. ROS inclula supmxidc mion. hydmryl 10% Igcia*rhmagar*.gnawinmmulsalin r;rlicll, hydncm pmai(k and r,aypcn ion, nll or which wing IWW hampim ml [he hmmwge~le WT ;I= u~w~ahk i(ld rnllly wwliw It1 9111~2 LJIv1m b Ik ~t111ri1ubpI 01 HIXI x 8 fir X) min at .I?. The .wp - huly mlting ill WOS mwmh lo subk sun* end IIuMl wa5 lgcd lor vnria. biachcmi mays. Raein ~10luuks~'~. When hu balm bawepl ROs and m- war eslimted by the mthd of lawyI1". Euimstions tioxibnt .spyrtar is losl ' oxidalive m' multsiq). Re- of auprmidc dLmdd8l. ~.ld'~). gluliuhim m. wliw ox&! wies has tun dawn to be inn44 in d~xnd"~ end hhydmBan -ids mian rcpylzlJ inlatilily due lo drreuive f ~ ~ l ' n and ~ " in ~ wac daa. 'he aivicies d -ic mzymcs 3p. ClyFlorchiistn a upm upmum! lo toxic &mi- hydmxyW rd 17phydmaysbroid Culs"','~J. I1 lu7 ha\n shown Ih hu (hc m dchdlqmm vsc eaimld irmrding 10 &gmyeozymrr 01 hu Semi&&' pathway ux mdccular oxy- dP1, The cxaCan md d.caminnciar or DNA end gen Jnd ekama mfu fmm NADR1 lo hydmxylnre RNA nwe CdWW M fdkwing lk lshique d the mh~nld"'. In chis pmxxs, rupmxide mion or ~chnidd"' Mher oxygen fm dials wre pmmad a e rcrult ci elecuon leakage in normal wlim or hr lo inlcracfion o f s t a v i d ~ ~ o r a with h hum- a ~ thelhcdmpmmprrnavdntts. Stalirialmlyzymt 1%) &r pviuur vmk ha% dawn rhol cndaulrvl sit wn &amd wing Stu(a('a ' I' test ~nsdomr["l. oltus tcui~ular furujons of pubcror mtst"' Md Signi~mnccordiff~uwse~~ pc0.05. mhxychla i- restive oxym ud &- u r n g,idiily~~ul ~ ~ m*i~,xkliull eri~yma in dull IWIO rab ~ ~ ~ i r W d a L 1 ) . T l r ~ 1 ~ ~ 1 ~ r o evalusreIhecllslofLindYronlk~~idru lh bqdy wigha or !inbae-lwcd rarq did rm spm and it? inn- on geroidoprsis in Ihc helaun. h a y n@fi dugesung cflk mamen. Ho*crrr. lhs bady &gku uar algniWy 2 Mamink and m e W ~.rlkendo~lkuamanammpndtoIhc ~ i n g ~ d m o(Ftgurr v I). d the ~ mi*ofdIbc,enb,,pllinl~)and vaunl pawc werr M dpifdy han thmc ofhumnPd~mupdninuh(T.MeI). Lildac (7-huphlaocyslohaae) was b giR fmn layakridm Rslicidg, Sah. Tmil Ndu, India. Andmsmmc 3, 17 dim, p-niuophcnyl -. p-niv o p h e n d M d d e l l 7 w - fmnSigmaUankaI6. (Sl.Louis, Mo.. USA). All thc drmblr ud wn. d mlylial gdc obW [nwn kwl m m b l aurEs. 2.2 ~Lrandrdrrrmmmr Wirvrmkmu(10-12wr+ktdgc)nweobwizd Cmn Ihc CaurJl Anid Fsiiily d Um lsWdllPl llrrll~lc or PBcgndujc WiI Eduoslm nd bmdl (JIPMER). Wichary. I*. Ths m .me mdnwlwd undn a wll-roguQtal li@ uJ darL (1%: IZh) ululukrZ4tR't~~~~~lr.aa~M crsiuy dm* and mp Wa. Lindrme M dial4 in o~~vc oil iu a amx~mtmirni or 5.0 464. ml Ike tau ~nwp W" giw> by crd iauhuritnl u( S i u a hap
ILXlll hnl, .I 121; 1.0 -/.a . Ill CinvrJ . - -.- -- ...- uuly wigla (8) nl * 16 1at9 Tcxw (111g) llXP t 50 &%$id I b .W5t10 SI~~,@ t$*I,"i~ (#I+) .ULltttB :2u + ah 0 I b . w l WJtW %*& Sonin*l &kd < 11%) (YL3*70 575 * Sb - hlal Wwwim Cuvol Twcd Slrpau.* Jlxnuslc' 23.41 t5.68 11.2ltZ.W ChILJ;lr' e.05+0.~5 1.n*0.ul1 Oldur~ n&w Ill .let 3.P U~.Iulf 2.7dk HPhiP w L5.16+0.05 ZI.GStZ.@ d - n my' Rucin' &5.25+0.3 38,16tO.& DNA' Z.PiO.11 O.B7oO.Lt? WAS 4.Wt0.01 3.46+0.l@ W W W ~ &).J)t8.(U 61.%3*2.YP M~y*lrln.l~ur* 17l~hyruwnul s,.a+d.a U.W*~.~JF ',,ml( mwnl~ wmaWmin pa nu pusin d 32F 5nd ycb, m-~.rvnlin p.r s. w#&n 6 IT. bs.4 NAWW ~xaa.V#9rn pr a&! ~ ld X?t. n Crrol~~,awna~n~,ponlspadn~~~. *hy( a &$I dm. 'brtml NAD 011tnnal 10 NMmin pa F a n d 32%. 1111;l1ion (WHO Repon) under lhc wagoly or whnchnlcal pmluCls a% being moJMlely hplnrdous Md it is still uscd arnpslicidcinmywwnuiw.. Adoseof5rngof liWkg body weight ha? been consi- by WHO LV No Chscrved Elk4 Level (NOEL). Bul in the present study the testis might8 of lindane-treated rat8 were significanlly dsueascd. Thc tcrlis hat been shown to be highly susceptible to lin& a8 it C- bid-testis h e r ilnd deprrsse ynmwlogemis["l. The d~cnose in the lcaticulor weight ul lindam-lrenled me my be due lo d u d Iubule sizc, spmatogenic m$t and inhib~lion 01 steroid biosynbis af Lcydig I l l g h . . Tiftt.fl 4W+& The mighe of cpididymis. wninnl vaicles md VLI*~~ Inuiuc. venml pwle ill lindnne-1mLed me wen decreased. Oll6) lUU*Pl IzutIP (m~ttllyb.~.) Ill taO 9)oP Sevual stdi have sham chat !he epididymis and ac- =\wry KX orgnns quire n wntinuws Yld~genic slimuintion fw vwntion ol their noml smcturnl and 7hc qxcific oelivilics of supemxi& dimuIn%. functid int~~ril$~'. l%us Lhe slight rtduction in the uwlw sul gluulhicne dubwc domd while the levels or hydmgm peroxide was found lo be elcvald in mlcd rats when mpand to the corrapcnding grwp of mlrol animals. 7hC wific oetivities of ~Midocenic weight ol che cpididymis and ncctasoly sex orgm in be vcated rats may be due 10 low bioavdlabilicy of andm- The mtioxidnnt sysm plays an errective role in arg~~xrr. :I&hyJruxywuid Jchydmgcw nnd I7khy. pmwcting Lcrm onl Mher biologicul tissu*; below a cricdruxyslwid kl~ydm~m~~w uue deumd signiliwrlly id lhrrxhvld or wdve oxygen specie? thus prrventilrg (P cO.a5). 'Iherr ms u sipiftam dswEpsc in the lev. Icrticulor dysl~nctionl''~. Anlioxidmt ciuymc$ constiUte cis of lcviculv DNA. RNA md win in mtal goup a rnudly suppoRive ltam or defence ngninsl Mctive W~II urn@ lo Ilr mud mim11I6 (ToMe 2). oxygen npcicr (RChS). In ~hc present study IhC acdviticq T.h*Z. of antioxidant wym, wpemride di.unulpsc, malase E i T a d l i n l v n m ~ ~ i n W e ~ o l ~nuknu, ,,=!a. A*S. bP< 1l.a vrlhccmrulp~up. iuvl glutnlhiw redwin.. were (!ecreuced in limlune.wl. ed me. Thc levels of hydmgen pmxide gencmlion were loud (o be elevaed which ma ar a h e r of in. crud ROS. Thus in he p m l study m increaw In hydrogen peroxide wilh a duction in snrioxidnnt cnzym idiw LhC.oxi&live Ues. induced hy lindime. Ihc dsreDsnl aivities of slemidogenic enrymer 3&hydmrysWuiJ dchydmpa~ and ll~hydmxystemid dehydrogenas were indicative of the duced Isticulx slemidopunsis, Lidme hns been shown to inhibit h lo thc puthqu'wis or li&r inducal rrpmlwtive dysfundionl". 7he Wiun 01 supruxide dint~ulw suggestis that it is involved in antioxidant defmce and it h;r$ ken Nwm~ to WI ns an aitcwa ngulalury swikb in lnticulilr nwidogelursidZ']. Ilr cyld~nulc PC41 alzymcs of Lhe sluuicbgenic plhway M known to produce rtte radiEals. Thesc free mdids M pmluad s s resull of elecm kSP.p due to Ik inlem~iun ol steroid pmJ- WL$ or & pwwbubxlmlc? wilh thc cnayllrcq. lhc inability of LhC ~ ~ lo be orygwtd l phmola e the relmse or wtive oxygen spie~(~"I. In lildururuld 1x1s IhC (!ecm in IhC teSli~vlx NnIelllS of
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FUNCTIONAL STUDIES ON THE EFFECTS O
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ACKNOWLEDGEMENTS I wish to express
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TABLE OF CONTENTS Page No ACKNOWLED
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TABLE OF CONTENTS (Continued) Deter
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TABLE OF CONTENTS (Continued) Effec
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TAULIC OF CON'I'ISN'I'S (C'untinued
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xi LIST OF TABLES (Continued) I'agc
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Table 24a Table 24b Table 24c Table
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Fig. 1 Fig. 2 Effect of TCDD on the
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Fig. 12c Fig. 12d Fig. 13 Fig. 14a
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LIST OF FIGURES (Continued) Fig. 23
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LH M PL mg mRNA N AD NADH NADP NADP
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layers, an outer layer of visceral
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tubular epithelium and continually
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lmn~unization of monkeys against FS
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and this reaction is catalyzed by t
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extratubular environment or synthes
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the most active in protein secretio
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1958). A rapid fall in serum testos
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have also been reported in the rats
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Effect of TCDD on heart muscle has
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1.4 Effect of oxidative stress on m
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2 SCOPE OF THE PRESENT STUDY There
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3 MATERIALS AND METHODS 3.1 Animals
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Subgroup III: Administration of TCD
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choppcd with the help of sharp razo
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anti-FSH antibody. After incubation
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lust sped (500 to 700 rpm) on an or
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3.13.4 Quantitative determinntiun u
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The plates were dried by inverting
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centrifuged at 3,500 g for I5 min o
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3.17 Preparation of tiasue humogena
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supcrnutant was taken. Hlunk was pr
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3.21.4 Assay of glutathione peroxid
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4 RESULTS 4.1 Administration of TCD
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seminiferous tubules was found to b
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fractions of rat testis as compared
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4.1.11.1 Effect of TCDD on the prod
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4.2 Co-administration of TCDD and v
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4.2.8 Effect of co-administration o
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4.2.11.5 Effect of co-administratio
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Table I b. Effect of TCDD on the we
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Table 4. Effect of TCDD on the seru
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Table 7b. Effect of TCDD on the pro
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Table 8c. Effect of TCDD on the lev
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Table 9d. Effect of TCDD on the act
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Table 13. Effect of TCDD on the ant
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Table 15. Effect of TCDD on the lev
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Table 16c. Effcct of TCDD on the an
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Table 18a. Effect of co-administrat
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Table 21. Effect of co-administrati
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Table 24b. Effect of co-administrat
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Table 25c. Effect of co-administrat
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Table 26d. Effect of co-administrat
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Table 30. Effect of co-administrati
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Table 32. Effect of caadrninistrati
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Table 33c. Effcct of co-administrut
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5 DISCUSSION 5.1 Significance of th
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Measurement of weights of the acces
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compartment of the cells (Chainy el
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the reduction in daily sperm produc
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eported in the TCDD-treated rats (M
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lilnction is to ililcrccpt lipid pc
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in the animals administered with TC
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protective effects on serum testost
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suygcstccl that vivamin 1: conld im
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Fig.4~. ENect of TCDD or TCDD and v
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tE W d In w it I I (,OCX) 3s PUB (,
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Fig. 7c. Effect of TCDD or TCDD and
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u 3 f ~ = m w * N O .- ~Slew aew en
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Fig.lOb. ENmt of TCDD or TCDD and v
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Fig. 1Ir Effect of TCDD or TCDD md
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Fig. 14c. Effect of TCDD or TCDD an
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Fig. 16b. Effect of TCDD or TCDD an
Page 164 and 165: Fig.18~. EKect of TCDD or TCDD and
Page 166 and 167: 1 1.2 l4 Fig. 19b. ENect of TCDD or
Page 168 and 169: Fig. ZOb. Elkt of TCDD or TCDD and
Page 171 and 172: Fig. 21. Effect olTCDD or TCDD and
Page 173 and 174: Fig. 22c. Effect of TCDD or TCDD an
Page 175 and 176: Fig. 24b. Eflect ofTCDD or TCDD and
Page 177 and 178: 7 REFERENCES Aitken RJ, Irvine DS,
Page 179 and 180: Chitm KC, Latchoumycandanc,C, Mathu
Page 181 and 182: Flsher JS, Turner KJ, Brown D, Shar
Page 183 and 184: Hornsby PJ. Regulation of cytochrom
Page 185 and 186: Latcboumycandane C, Chitra KC, Math
Page 187 and 188: Moore RW, Panona JA, Bookstaff RC,
Page 189 and 190: Rommerts FFG, Cooke BA, Van Der Kem
Page 191 and 192: Slaunwhite WR, Burgett MJ. h virro
Page 193 and 194: 1.1 Preparation of dosing solution
Page 195 and 196: 1.4.6 Wash concentrate One bottle,
Page 197 and 198: 1.7.3 Estradiol controls Two vials.
Page 199 and 200: 1.1 1.2 Orcinol(6%) 6 g of orcinol
Page 201 and 202: 1.16.2 Horseradish peroxidase (8 Un
Page 203 and 204: 1.20 Superoxide dismutase 1.20.1 Tr
Page 205 and 206: 1.24.2 Thiobarbituric acid (TBA; 0.
Page 207 and 208: Chronic effect: of enclosulfan on t
Page 209: enbw1h1.W mu IUY Wcab unpiliml~t at
Page 214 and 215: DNA, RNA ;uxl Wcin wcrr abarval, wh
Page 216 and 217: Mt~~m(10-lZsu&)vacob- Wfmn Ihehml ~
Page 218 and 219: New Delhi, llrliil Ib SCI~KK Rcm& M
Page 220 and 221: tiii~lc rcprmluction or rut (Cllitr
Page 222 and 223: wciglit (b.wt.) per day for 45 days
Page 224 and 225: 0.1 00 01 " 0.8 I . .lo Dl* rcnn IW
Page 226 and 227: Chilr:~. K.C.. L!olnn!myci!~dum. C.
Page 228 and 229: C. hteboumyeradlos . KC. CMtn . P.P
Page 230 and 231: I. 1 Ilal 01 2 1.7 # I c I ~ ~ L I
Page 232 and 233: Fin. L Corrclatwn bclwfcn "perm cou
Page 235: Iionshii nepmdvctivc mxicity ad upt
Page 240 and 241: o. Glutathione reductage mg pmWn mg
Page 242 and 243: IOWAME -. Tor. #558517 PME: 9 SESS:
Page 244 and 245: JOBNAME: Rqm. Tm. M5W7 PAOB: 11 SBS
Page 246 and 247: t 02 ELSEVIER Toricology OM) (2W1)
Page 248 and 249: ---- - wcre obtu~ncd from E-Monk. G
Page 250: 3.2. A,III~AILNI syrlo~t of ,nIroch
Page 253 and 254: Uc,#!dy. SC. N;tdcru. S , 1% Conlrb
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