ON TESTIS AND EPlDlDYMlS OF RATS - Pondicherry University ...

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Effect of lindane on testicular antioxidant system and steroidogenic enzymes in adult rats K. Sujeh. K.C. ChiVd, C. L utchoumyct, P.P. Mahur *hd MLp 5clcnw, Pndrclvny Un,uniIy, Pmdichny 6% ill4 . Ida Abreact &: To fird wl hc efiozl of lib on Ic~iiculw arlioxihl system nnd lesiiculer rlcmidupmsis in dull male r.B. Methods: Adult male rats wus orally dminislued wilh iindan at a dm of 5.0 rng/kg body wighl per day fiu RO duyr. Twenty-[our hours nRCr LhC 13Y Mllmenl Ulc mLs w a kilkd ~ using n d i c &r. Testes, cpididymis. wnlinttl v~%iclc% ed wtml pmtake wcrr mwd nnd wigM. A 10% (alicuk lrgenute wa prcpDlsd ud mn- 1lirug4 nl ,IT. Ihc wpcmacun~ w 4 tor biwlmn~wl eslimntions. Results: 'Ihe buly wiyhl MII he weights of ~sler, qdddymis, reminal &la end vend p w ware red& in limhw-Mlllcd mls. Then was a rignikm klinc in rhc Daivilia of Mlioxhnt cmyms mpuoxidc dismutasc (SOD). nnd glubthiar Rduc- L= dlik cut it- in hfi- pcmxide (YO,) gencmlion wa* churned. 7he spwiiic activilie% or Lerlicular urnihqar av.ria:+hrJRUwd ddlyil~~$uwp nnd llp-hydmxyslemid dehydquwsc wn 4324. Tk kuslr o( DNA. RNA anl pmdn wcrr alsn dsnased in lindmr-vmtcd ram. Conclusion: Lindme indum oxi&- liw.mxrMddoourstaPntiO*dPIIf~~~~in&ltmalcm~. (kiv1JAnlml2WI: 3: 135-138) V+ on rqxodunive functions in wildlife ad hunw~"~. Lin- 1 InmJdvction dam has ban pviwly shown to muse loxic cfiozls on The orpwchlolinc insmid& lih ( 7-hu- tcrres of m. In fanulc rats li& exem anlialm- Irhlorocyc~) is widcly 4 m a pacicidc in genic sctivity by diwping ulmus cycle with a reduction m y muwria. In India it b widcly used in agriculhln in rhc ulaine vhi$~[~). Lindsm hss been rcponod to al- Md puhlic MUI 7. Lindm WUWx lcci rhc dcvelymeru d MLW crnbv in vifm in e lip*bilk: ClPMQ MJ enm Inlo Ur food chin mull- dosbdcpndcnl rmnd'). ing in bbmmdmim in Iho kdy Iixue6, Mood rind L i h when given orally lo mia during wrly, brrwtmiikofhuwrMdrvi~~ilc"~. L i h d o k mid Md lalo ppmm& hna been repfled lo muse IMal nlxac of imlnnmtion aim, locnl rcfiaption of fHusC.. d low binh'weighc of plp. wilh M i-v in number n*'adoaitm &n ad my cut dvslst of dad faw, rrspclinly[", Thc m1m arc highly nnaplib* D lindm r It the blaod-ds bsrricr Cl%-al a. P.P. k(l.h.. SkddWa%m=. ud with s n d c Rduction . IWdq WW. RwwImYaw. ha. Td, + P14~2lZ Rr, +9lW912 Ed. ~ O p . p a . d c . h o ~ O ~ . u a \ YIWW SILI-17 rntllwl
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FUNCTIONAL STUDIES ON THE EFFECTS O
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ACKNOWLEDGEMENTS I wish to express
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TABLE OF CONTENTS Page No ACKNOWLED
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TABLE OF CONTENTS (Continued) Deter
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TABLE OF CONTENTS (Continued) Effec
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TAULIC OF CON'I'ISN'I'S (C'untinued
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xi LIST OF TABLES (Continued) I'agc
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Table 24a Table 24b Table 24c Table
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Fig. 1 Fig. 2 Effect of TCDD on the
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Fig. 12c Fig. 12d Fig. 13 Fig. 14a
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LIST OF FIGURES (Continued) Fig. 23
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LH M PL mg mRNA N AD NADH NADP NADP
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layers, an outer layer of visceral
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tubular epithelium and continually
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lmn~unization of monkeys against FS
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and this reaction is catalyzed by t
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extratubular environment or synthes
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the most active in protein secretio
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1958). A rapid fall in serum testos
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have also been reported in the rats
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Effect of TCDD on heart muscle has
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1.4 Effect of oxidative stress on m
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2 SCOPE OF THE PRESENT STUDY There
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3 MATERIALS AND METHODS 3.1 Animals
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Subgroup III: Administration of TCD
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choppcd with the help of sharp razo
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anti-FSH antibody. After incubation
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lust sped (500 to 700 rpm) on an or
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3.13.4 Quantitative determinntiun u
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The plates were dried by inverting
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centrifuged at 3,500 g for I5 min o
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3.17 Preparation of tiasue humogena
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supcrnutant was taken. Hlunk was pr
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3.21.4 Assay of glutathione peroxid
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4 RESULTS 4.1 Administration of TCD
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seminiferous tubules was found to b
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fractions of rat testis as compared
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4.1.11.1 Effect of TCDD on the prod
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4.2 Co-administration of TCDD and v
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4.2.8 Effect of co-administration o
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4.2.11.5 Effect of co-administratio
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Table I b. Effect of TCDD on the we
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Table 4. Effect of TCDD on the seru
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Table 7b. Effect of TCDD on the pro
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Table 8c. Effect of TCDD on the lev
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Table 9d. Effect of TCDD on the act
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Table 13. Effect of TCDD on the ant
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Table 15. Effect of TCDD on the lev
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Table 16c. Effcct of TCDD on the an
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Table 18a. Effect of co-administrat
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Table 21. Effect of co-administrati
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Table 24b. Effect of co-administrat
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Table 25c. Effect of co-administrat
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Table 26d. Effect of co-administrat
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Table 30. Effect of co-administrati
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Table 32. Effect of caadrninistrati
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Table 33c. Effcct of co-administrut
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5 DISCUSSION 5.1 Significance of th
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Measurement of weights of the acces
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compartment of the cells (Chainy el
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the reduction in daily sperm produc
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eported in the TCDD-treated rats (M
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lilnction is to ililcrccpt lipid pc
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in the animals administered with TC
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protective effects on serum testost
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suygcstccl that vivamin 1: conld im
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Fig.4~. ENect of TCDD or TCDD and v
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tE W d In w it I I (,OCX) 3s PUB (,
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Fig. 7c. Effect of TCDD or TCDD and
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u 3 f ~ = m w * N O .- ~Slew aew en
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Fig.lOb. ENmt of TCDD or TCDD and v
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Fig. 1Ir Effect of TCDD or TCDD md
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Fig. 14c. Effect of TCDD or TCDD an
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Page 164 and 165: Fig.18~. EKect of TCDD or TCDD and
Page 166 and 167: 1 1.2 l4 Fig. 19b. ENect of TCDD or
Page 168 and 169: Fig. ZOb. Elkt of TCDD or TCDD and
Page 171 and 172: Fig. 21. Effect olTCDD or TCDD and
Page 173 and 174: Fig. 22c. Effect of TCDD or TCDD an
Page 175 and 176: Fig. 24b. Eflect ofTCDD or TCDD and
Page 177 and 178: 7 REFERENCES Aitken RJ, Irvine DS,
Page 179 and 180: Chitm KC, Latchoumycandanc,C, Mathu
Page 181 and 182: Flsher JS, Turner KJ, Brown D, Shar
Page 183 and 184: Hornsby PJ. Regulation of cytochrom
Page 185 and 186: Latcboumycandane C, Chitra KC, Math
Page 187 and 188: Moore RW, Panona JA, Bookstaff RC,
Page 189 and 190: Rommerts FFG, Cooke BA, Van Der Kem
Page 191 and 192: Slaunwhite WR, Burgett MJ. h virro
Page 193 and 194: 1.1 Preparation of dosing solution
Page 195 and 196: 1.4.6 Wash concentrate One bottle,
Page 197 and 198: 1.7.3 Estradiol controls Two vials.
Page 199 and 200: 1.1 1.2 Orcinol(6%) 6 g of orcinol
Page 201 and 202: 1.16.2 Horseradish peroxidase (8 Un
Page 203 and 204: 1.20 Superoxide dismutase 1.20.1 Tr
Page 205 and 206: 1.24.2 Thiobarbituric acid (TBA; 0.
Page 207 and 208: Chronic effect: of enclosulfan on t
Page 209: enbw1h1.W mu IUY Wcab unpiliml~t at
Page 213 and 214: ILXlll hnl, .I 121; 1.0 -/.a . Ill
Page 215 and 216: Effect of lindane on antioxidant en
Page 217 and 218: WnCr, inyminn~nt of spmri motility,
Page 219 and 220: The effect of 2,3,7,8-tetrachlorodi
Page 221 and 222: I kl
Page 223 and 224: C'. I~~I~~~~IIIII~~O~,~~~~~~ R6+ 4,
Page 225 and 226: 'lstrr,tb~xr 171 i?lYJ?I 127 /.IS I
Page 227 and 228: S.tlc. S.. ?IXII. Mubuli~r bioluyy
Page 229 and 230: (I't,l:~t~cl 211x1 Kin~hronglt IVX4
Page 231 and 232: t11.3. Wal 01 TCDD on !he acttnly o
Page 233: IIR h r k a ~ r Ilw ~ ;njllmr~ l ~
Page 239 and 240: C L m r ~ n . l l ~ T a d ~ 1 6 W J
Page 241 and 242: c urbmwen&n= s a( I n.pradpradpradn
Page 243 and 244: - aaarslu30 ,.*mra. L~jl;lr; -P4-w~
Page 245 and 246: (=I 23. w- C Olln (LC W W. Ubrth d-
Page 247 and 248: continuous exposure of humans to in
Page 249 and 250: pH 7.6). I00 pl of 200 mM NADPH, IW
Page 252 and 253: ioavaiiability andlor production of
Page 254: Glutnchlone irrox$dssc, rupcioridc
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ON TESTIS AND EPlDlDYMlS OF RATS - Pondicherry University ...

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