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Yoshida - 1981 - Fundamentals of Rice Crop Science

Yoshida - 1981 - Fundamentals of Rice Crop Science

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NUTRITIONAL DISORDERS 187<br />

Table 4.8. Methods for available soil nutrients. a<br />

Nutrient<br />

Methods <strong>of</strong> analysis<br />

N<br />

P<br />

Incubation method<br />

Bray-Kurtz No. 2 method<br />

K<br />

Exchangeable form<br />

Si<br />

1N Na-acetate (pH 4.0) extraction<br />

B<br />

Hot water<br />

Cu DTPA + CaCI 2 (pH 7.3)<br />

Fe DTPA – CaCI 2 (pH 7.3)<br />

NH 4 -acetate (pH 4.8)<br />

Mn DTPA + CaCI, (pH 7.3)<br />

0.1 N H 3 PO 4 and 3 N NH 4 H 2 PO 4<br />

Mo NH 4 -oxalate (pH 3.3)<br />

Zn<br />

0.05 N HCI<br />

Dithizone + NH 4 -acetate<br />

EDTA + (NH 4 ) 2 CO 3<br />

DTPA + CaCI 2 (pH 7.3)<br />

a Kawaguchi and Kyuma (1977) for macronutrients; Randhawa et<br />

al (1978) for micronutrients.<br />

band to a chlorotic leaf. Within a week, the band will become green if the chlorosis<br />

is due to iron deficiency. For suspected zinc deficiency, 1 or 2 plants may be<br />

sprayed with a 0.1% aqueous solution <strong>of</strong> zinc sulfate or zinc chloride with a small<br />

amount <strong>of</strong> sticker. If the symptoms are due to zinc deficiency, the plant will begin<br />

to recover in a week or so.<br />

4.4.2. Greenhouse experiment<br />

A small amount <strong>of</strong> soil may be collected from the problem field and tested under<br />

controlled conditions in the greenhouse for various means <strong>of</strong> correcting the<br />

problem.<br />

Potted conditions in the greenhouse may differ from field conditions. For<br />

example, pot experiments in the greenhouse occasionally fail to reproduce symptoms<br />

while the problem field suffers severe zinc deficiency. In such a case, the<br />

addition <strong>of</strong> cellulose powder assures the reproduction <strong>of</strong> zinc deficiency by<br />

aggravating the problem (<strong>Yoshida</strong> and Tanaka 1969).<br />

4.4.3. Field experiment<br />

A final assessment <strong>of</strong> the diagnosis and the practical means <strong>of</strong> correcting the<br />

problem must be tested at the problem site. Various forms and amounts <strong>of</strong><br />

chemicals, and methods and times <strong>of</strong> application can be tested by the field<br />

experiment (<strong>Yoshida</strong> et al 1971, 1973).<br />

The diagnosis is assumed to be correct when the treatment corrects the problem.<br />

The cause-effect relationship is thus established. This may not always be true,<br />

however. In the alkali soil areas <strong>of</strong> California, a severe chlorosis called alkali<br />

disease occurs on water-sown rice. The symptoms are indicative <strong>of</strong> iron deficiency<br />

caused by high pH. In field experiments, the application <strong>of</strong> ferric sulfate was

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