BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
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affinity Chromatography<br />
Protein Fractionation of Complex Samples on the mRP <strong>Column</strong><br />
4.6 x 50 mm mRP-C18<br />
mAU<br />
7<br />
6<br />
5<br />
4<br />
3<br />
2<br />
1<br />
0<br />
mAU<br />
25<br />
20<br />
15<br />
10<br />
5<br />
0<br />
0<br />
10 20 30 40 50 60 70 80<br />
Time (min)<br />
HeLa Membrane Prep<br />
10 20 30<br />
Time (min)<br />
40 50<br />
"Top-6" Depleted Human Serum<br />
9 www.agilent.com/chem/biohplc<br />
Highest Recovery<br />
mAU<br />
8<br />
7<br />
6<br />
5<br />
4<br />
3<br />
2<br />
1<br />
0<br />
0<br />
mAU<br />
25<br />
20<br />
15<br />
10<br />
5<br />
0<br />
0<br />
10 20 30<br />
Time (min)<br />
40 50 60<br />
HeLa Cell Lysate (352 µg)<br />
10 20 30 40 50 60<br />
Time (min)<br />
Lipids<br />
Human Brain Membrane Lipid Raft Prep (500 µg)<br />
MRPC18
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