BioHPLC Column Selection Guide Cover - Agilent Technologies

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Prep to Process PLRP-S 126 www.agilent.com/chem/biohplc PLRP-S Bulk Media Particle Size (µm) Unit PLRP-S 100Å PLRP-S 200Å PLRP-S 300Å PLRP-S 1000Å 50 1 kg PL1412-6K00 PL1412-6K05 PL1412-6K01 PL1412-6K02 100 g PL1412-4K00 PL1412-4K05 PL1412-4K01 PL1412-4K02 PLRP-S 4000Å 30 1 kg PL1412-6702 PL1412-6703 100 g PL1412-4702 PL1412-4703 15-20 1 kg PL1412-6200 PL1412-6201 100 g PL1412-4200 PL1412-4201 10-15 1 kg PL1412-6400 PL1412-6401 100 g PL1412-4400 PL1412-4401 10 1 kg PL1412-6100 PL1412-6101 PL1412-6102 PL1412-6103 100 g PL1412-4100 PL1412-4101 PL1412-4102 PL1412-4103 8 1 kg PL1412-6800 PL1412-6801 For larger quantities, please contact your local <strong>Agilent</strong> sales office.
PL-SAX AND PL-SCX FOR PREP TO PROCESS Prep to Process PL-SAX and PL-SCX • Ion-exchange purifications over a wider pH range extend applications • HPLC flow rates and rapid equilibration reduce purification cycle times • Large pore size for improved mass transfer delivers high speed, high resolution purifications These rigid, strong ion-exchange materials are extremely hydrophilic and are designed for purification of biomolecules. The PL-SAX and PL-SCX materials are totally polymeric and are chemically and thermally stable over a full range of HPLC conditions. The strong ion-exchange functionalities, covalently linked to a chemically stable polymer, facilitate ion-exchange purifications over a wider pH range. This stability can be exploited for column sanitation and clean-up. Thermal stability also enables the use of denaturing conditions and stabilizing/solubilizing agents for the purification of target compounds, as encountered in the purification of synthetic oligonucleotides with self-complementary sequences. Both the 1000Å and 4000Å wide-pore materials are mechanically stable and robust and can be operated over a wide range of linear velocities, with fast loading of dilute solutions and wash cycles. HPLC flow rates and rapid equilibration reduces purification cycle times. Packing in dynamic axial compression (DAC) column hardware is straightforward and high efficiency columns are achieved with excellent reproducibility and lifetimes. The 1000Å pore size is for high-capacity purifications and the 4000Å gigaporous particles with improved mass transfer are intended for large biomolecules and high-speed, high-resolution purifications. PURIFICATION 127
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Agilent BioHPLC Column Selection Gu
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BIOCOLUMN SELECTION GUIDELINES From
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Column Selection Flow Chart Biocolu
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Protein Column Selection Guide Appl
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Separation of charge variants of hu
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Increased resolution for peptide ma
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Preparative scale purification of L
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DNA and RNA Oligonucleotide Column
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Broad Distribution Biomolecules Car
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ReveRseD-PHase HPLC Confidently per
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Sterically Protected 300StableBond
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Improved reproducibility of monoclo
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Short-chain ZORBAX 300SB-C3 is stab
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ZORBAX 300Å StableBond Hardware De
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LC/MS analysis of angiotensin on Ex
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Tips & Tools ZORBAX 300Å Extend-C1
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Poroshell 300 columns separate prot
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Monoclonal IgG1 chains: Separation
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Poroshell 120 • 120Å pore size f
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0 µm 0 µm 0 µm A B C Scanning el
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Exploiting chemical stability - TFA
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Temperature as a tool to enhance ma
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ZORBaX amino acid analysis (aaa) Co
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High resolution of 24 amino acids u
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Ion-Exchange Column Selection Appli
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Consistent ion-exchange MAb separat
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Column Specifications Tips & Tools
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Weak cation-exchange chromatography
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PL-SAX Strong Anion-Exchange Column
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Analysis of representative whey pro
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Standard protein separation Column:
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Agilent Bio-Monolith HPLC Column Se
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Baseline expansion of a separation
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size exclusion Chromatography (seC)
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Column Specifications Agilent Bio S
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Comparison of Agilent Bio SEC-3 and
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Column Length size exclusion Chroma
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Column Specifications Agilent Bio S
Page 77 and 78: Calibration curves - Bio SEC-5 Colu
Page 79 and 80: ProSEC 300S • Mechanically robust
Page 81 and 82: Overlay of UV and light scattering
Page 83 and 84: Retention volume versus log (MW) fo
Page 85 and 86: Agilent PL aquagel-OH SEC Columns f
Page 87 and 88: Heparin Column: 2 x PL aquagel-OH 3
Page 89 and 90: Polyethylene Glycol/Oxide standards
Page 91 and 92: aFFiniTY CHROMaTOGRaPHY Agilent Bio
Page 93 and 94: Rapid human polyclonal IgG quantifi
Page 95 and 96: Multiple Affinity Removal System af
Page 97 and 98: Illustration of high abundance prot
Page 99 and 100: mRP-C18 High-Recovery Protein Colum
Page 101 and 102: MeTHOD DeveLOPMenT ZORBAX Column Me
Page 103 and 104: Reversed-Phase LC/MS Methods Method
Page 105 and 106: Method Development Ionic Strength:
Page 107 and 108: Method Development After the initia
Page 109 and 110: Tips & Tools Agilent offers a varie
Page 111 and 112: Human serum: Low abundance protein
Page 113 and 114: 2-D LC/MS Analyses Using ZORBAX Cap
Page 115 and 116: ZORBAX Bio-SCX Series II Capillary
Page 117 and 118: ZORBAX HPLC Capillary Columns (glas
Page 119 and 120: Microbore HPLC for sensitive peptid
Page 121 and 122: BioPharmaceutical Lifecycle Reverse
Page 123 and 124: ZORBAX 300Å StableBond ZORBAX Prep
Page 125 and 126: PLRP-S Prep to Process Application
Page 127: Prep to Process PLRP-S Size (mm) Pa
Page 131 and 132: Prep to Process PL-SAX and PL-SCX D
Page 133 and 134: Crude peptide screen Column: VariTi
Page 135 and 136: CARTRIDGE COLUMN SYSTEMS Cartridge
Page 137 and 138: USP DESIGNATIONS - BIOHPLC COLUMNS
Page 139 and 140: BioHPLC Columns Literature Title Ag
Page 141 and 142: BioHPLC Columns Literature Title An
Page 143 and 144: BioHPLC Columns Literature Title Co
Page 145 and 146: BIOPHARM DEFINITIONS A active start
Page 147 and 148: dimer A polymer made up of two iden
Page 149 and 150: K-L ligase An enzyme that causes mo
Page 151 and 152: S secondary structure In proteins,
Page 153 and 154: Poroshell 300 Citations Matilainen,
Page 155 and 156: Agilent 1260 Infinity Bio-inert Qua
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BioHPLC Column Selection Guide Cover - Agilent Technologies

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