BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
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ion-exchange Chromatography<br />
Standard ion-exchange protein separation<br />
<strong>Column</strong>: PL-SAX 1000Å<br />
PL1551-1502<br />
4.6 x 50 mm, 5 µm<br />
Mobile Phase: A: 10 mM Tris HCl pH 8<br />
B: A+0.35 M NaCl pH 8<br />
Gradient: 0-100% B in 20 min<br />
Flow Rate: 1.0 mL/min<br />
Detector: UV, 220 nm<br />
Analysis of choline kinase<br />
on PL-SAX 4000Å<br />
<strong>Column</strong>: PL-SAX<br />
PL1551-1803<br />
4.6 x 50 mm, 8 µm<br />
Mobile Phase: A: 20 mM Tris 5% ethylene<br />
glycol, pH 7.5<br />
(The following are required<br />
to retain enzyme activity)<br />
1.0 mM Ethylene glycol<br />
tetraacetic acid<br />
2.0 mM ß-Mercaptoethanol<br />
0.2 mM Phenylmethylsulfonyl<br />
fluoride<br />
B: A + 1 M KCI<br />
Flow Rate: 3.0 mL/min<br />
Detector: UV, 280 nm<br />
Separation courtesy of T Porter, Purdue University, USA<br />
5 www.agilent.com/chem/biohplc<br />
1<br />
2<br />
3<br />
0 min<br />
25<br />
VLC0070<br />
4<br />
enzyme activity<br />
1. Myoglobin<br />
2. Bovine carbonic anhydrase<br />
3. Ovalbumin<br />
4. Soybean trypsin inhibitor<br />
0 min 40<br />
%B<br />
100<br />
0
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