BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
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USP DESIGNATIONS – BIOHPLC COLUMNS ONLY<br />
For a full listing of USP designations for all HPLC columns, please consult the <strong>Agilent</strong> catalog or www.agilent.com.<br />
The US Pharmacopeia (USP) is a standard source for many pharmaceutical methods that specifies columns by packing materials rather than<br />
by manufacturer. Listed below are the recommended <strong>Agilent</strong> <strong>Technologies</strong> columns suitable for most LC methods listed with the USP.<br />
Appendices<br />
USP Method USP Packing Materials <strong>Column</strong> Particle Size (µm)<br />
L1 Octadecyl silane chemically bonded to porous silica or ceramic micro-particles, Poroshell 120 EC-C18 2.7<br />
1.5 to 10 µm in diameter<br />
Poroshell 120 SB-C18 2.7<br />
ZORBAX 300SB-C18 1.8, 3.5, 5, 7<br />
ZORBAX 300 Extend C18 3.5, 5<br />
L7 Octyl silane chemically bonded to totally porous microsilica particles,<br />
Poroshell 120 EC-C8 2.7<br />
1.5 to 10 µm in diameter<br />
ZORBAX 300SB C-8 1.8, 3.5, 5, 7<br />
L10 Nitrile groups chemically bonded to porous silica particles, 3 to 10 µm in diameter ZORBAX 300 SB-CN 3.5, 5, 7<br />
L14 Silica gel 10 µm in diameter with a chemically bonded, strongly basic quaternary<br />
ammonium anion-exchange coating<br />
ZORBAX SAX 5<br />
L17 Strong cation-exchange resin consisting of sulfonated cross-linked<br />
Hi-Plex H 8<br />
styrene-divinylbenzene copolymer in the hydrogen form, 7 to 11 µm in diameter<br />
Bio SCX NP10 10<br />
L21 A rigid, spherical styrene-divinylbenzene copolymer, 5 to 10 µm in diameter PLRP-S 100Å 3, 5, 8, 10<br />
PLRP-S 300Å 3, 5, 8, 10<br />
PLRP-S 1000Å 5, 8, 10<br />
PLRP-S 4000Å 5, 8, 10<br />
L25 Packing having the capacity to separate compounds with a MW range from<br />
1,000 to 5,000 da (as determined by the polyethylene oxide), applied to neutral,<br />
ionic and cationic water-soluble polymers<br />
PL aquagel-OH 5, 8<br />
L33 Packing having the capacity to separate proteins by molecular size over a range ZORBAX GF-250 4<br />
of 4,000 to 400,000 da. It is spherical, silica-based, and processed to provide<br />
pH stability<br />
Bio SEC-3 3<br />
Bio SEC-5 5<br />
ProSEC 300S 5<br />
L35 A zirconium-stabilized spherical silica packing with a hydrophilic (diol-type) ZORBAX GF-250 4<br />
molecular monolayer bonded phase<br />
ZORBAX GF-450 6<br />
L50 A strong cation-exchange resin made of porous silica with sulfopropyl groups,<br />
5 to 10 µm in diameter<br />
ZORBAX 300SCX 5<br />
L56 Propyl silane chemically bonded to totally porous silica particles,<br />
3 to 10 µm in diameter<br />
ZORBAX SB-C3 1.5, 3, 5<br />
APPENDICES<br />
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