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BioHPLC Column Selection Guide Cover - Agilent Technologies

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Capillary and Nano <strong>Column</strong>s<br />

ZORBAX Bio-SCX Series II<br />

provides more retention of small peptides<br />

<strong>Column</strong>: ZORBAX Bio SCX Series II<br />

5065-9912<br />

0.3 x 35 mm, 3.5 µm<br />

Mobile Phase: 95% 40 mM NaCl: 5% ACN,<br />

0.3% Formic acid<br />

Flow Rate: 5 µL/min<br />

Detector: 230 nm<br />

Sample: Synthetic Dipeptides<br />

114 www.agilent.com/chem/biohplc<br />

mAU<br />

40<br />

20<br />

0<br />

1<br />

2<br />

1. Trp - Tyr<br />

2. Mct - Trp<br />

0 2<br />

4<br />

6 8 10<br />

Time (min)<br />

mAU<br />

40<br />

20<br />

0<br />

1 2<br />

0 2<br />

4<br />

6 8 10<br />

Time (min)<br />

The new ZORBAX Bio-SCX Series II column retains smaller peptides more strongly than some other SCX columns. The result is increased<br />

resolution of more hydrophilic peptides fragments and more accurate identification when these columns are used in 2-D HPLC analysis.<br />

LCIE002

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