BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
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Capillary and Nano <strong>Column</strong>s<br />
ZORBAX Bio-SCX Series II<br />
provides more retention of small peptides<br />
<strong>Column</strong>: ZORBAX Bio SCX Series II<br />
5065-9912<br />
0.3 x 35 mm, 3.5 µm<br />
Mobile Phase: 95% 40 mM NaCl: 5% ACN,<br />
0.3% Formic acid<br />
Flow Rate: 5 µL/min<br />
Detector: 230 nm<br />
Sample: Synthetic Dipeptides<br />
114 www.agilent.com/chem/biohplc<br />
mAU<br />
40<br />
20<br />
0<br />
1<br />
2<br />
1. Trp - Tyr<br />
2. Mct - Trp<br />
0 2<br />
4<br />
6 8 10<br />
Time (min)<br />
mAU<br />
40<br />
20<br />
0<br />
1 2<br />
0 2<br />
4<br />
6 8 10<br />
Time (min)<br />
The new ZORBAX Bio-SCX Series II column retains smaller peptides more strongly than some other SCX columns. The result is increased<br />
resolution of more hydrophilic peptides fragments and more accurate identification when these columns are used in 2-D HPLC analysis.<br />
LCIE002