BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
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Polyethylene Glycol/Oxide standards<br />
<strong>Column</strong>: PL aquagel-OH MIXED-H 8 µm<br />
PL1149-6800<br />
7.5 x 300 mm, 8 µm<br />
Mobile Phase: Water<br />
Flow Rate: 1.0 mL/min<br />
Detector: <strong>Agilent</strong> PL-GPC 50 (RI)<br />
Standards for Biomolecule Analysis –<br />
Polyethylene Glycol/Oxide<br />
Most popular standards for aqueous SEC<br />
size exclusion Chromatography (seC)<br />
• Available in individual, kit, and EasiVial formats<br />
• Combine glycols and oxides to extend the MW range and cover more applications<br />
• MWs selected to provide equidistant calibration points for greater accuracy<br />
These hydrophilic polymers are both suitable for aqueous SEC. The oxides are available in high<br />
molecular weights, while the glycols cover the lower molecular weight range.<br />
1<br />
Specifications<br />
1<br />
2<br />
Retention time/min<br />
3<br />
4<br />
5<br />
11<br />
VLC0041<br />
EasiVial Color EasiVial PEG/PEO EasiVial PEG<br />
Red 600 282<br />
12,000 1,000<br />
125,000 6,000<br />
1,200,000 35,000<br />
Yellow 200 194<br />
4,000 600<br />
60,000 3,750<br />
1,000,000 21,000<br />
Green 100 106<br />
1,500 420<br />
25,000 2,000<br />
460,000 12,000<br />
1. 1,702,000<br />
2. 120,000<br />
3. 12,600<br />
4. 1,470<br />
5. 106<br />
UHPLC/HPLC