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BioHPLC Column Selection Guide Cover - Agilent Technologies

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Polyethylene Glycol/Oxide standards<br />

<strong>Column</strong>: PL aquagel-OH MIXED-H 8 µm<br />

PL1149-6800<br />

7.5 x 300 mm, 8 µm<br />

Mobile Phase: Water<br />

Flow Rate: 1.0 mL/min<br />

Detector: <strong>Agilent</strong> PL-GPC 50 (RI)<br />

Standards for Biomolecule Analysis –<br />

Polyethylene Glycol/Oxide<br />

Most popular standards for aqueous SEC<br />

size exclusion Chromatography (seC)<br />

• Available in individual, kit, and EasiVial formats<br />

• Combine glycols and oxides to extend the MW range and cover more applications<br />

• MWs selected to provide equidistant calibration points for greater accuracy<br />

These hydrophilic polymers are both suitable for aqueous SEC. The oxides are available in high<br />

molecular weights, while the glycols cover the lower molecular weight range.<br />

1<br />

Specifications<br />

1<br />

2<br />

Retention time/min<br />

3<br />

4<br />

5<br />

11<br />

VLC0041<br />

EasiVial Color EasiVial PEG/PEO EasiVial PEG<br />

Red 600 282<br />

12,000 1,000<br />

125,000 6,000<br />

1,200,000 35,000<br />

Yellow 200 194<br />

4,000 600<br />

60,000 3,750<br />

1,000,000 21,000<br />

Green 100 106<br />

1,500 420<br />

25,000 2,000<br />

460,000 12,000<br />

1. 1,702,000<br />

2. 120,000<br />

3. 12,600<br />

4. 1,470<br />

5. 106<br />

UHPLC/HPLC

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