BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
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<strong>Column</strong> Length<br />
size exclusion Chromatography (seC)<br />
Where the separation time is a critical parameter shorter columns packed with the higher efficiency 3 µm media are used. With the shorter<br />
columns higher flow rates are used to reduce the analysis time but without compromising the quality of the data – quantitation of monoclonal<br />
antibody monomer and dimer.<br />
<strong>Agilent</strong> Bio SEC-3 column length comparison, 150 mm<br />
<strong>Column</strong>: Bio SEC-3, 300Å<br />
5190-2512<br />
7.8 x 150 mm, 3 µm<br />
Mobile Phase: 150 mM sodium phosphate<br />
Flow Rate: 1.0 mL/min (56 bar), 1.5 mL/min (75 bar)<br />
Detector: 220 nm<br />
Sample: MAb (2 mg/mL)<br />
150 mm 300 mm<br />
1.5 mL/min 1.0 mL/min<br />
Monomer<br />
Dimer<br />
Monomer<br />
Dimer<br />
4 min<br />
<strong>Agilent</strong> Bio SEC-3 column length comparison, 300 mm<br />
<strong>Column</strong>: Bio SEC-3, 300Å<br />
5190-2511<br />
7.8 x 300 mm, 3 µm<br />
Mobile Phase: 150 mM sodium phosphate + 100 mM Na sulfate (with salt)<br />
150 mM sodium phosphate (without salt)<br />
Flow Rate: 1.0 mL/min<br />
Detector: 220 nm<br />
Sample: MAb (2 mg/mL)<br />
With salt<br />
Without salt<br />
Monomer<br />
Dimer<br />
8 min<br />
UHPLC/HPLC
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