BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
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Overlay of UV and light scattering 90º<br />
for a sample of γ-globulins, illustrating<br />
monomer, dimer, and trimer peaks<br />
<strong>Column</strong>: ProSEC 300S<br />
PL1147-6501<br />
7.5 x 300 mm, 5 µm<br />
Mobile Phase: 0.1 M KH 2PO 4 containing 0.3 M NaCl, pH 8.0<br />
Flow Rate: 1.0 mL/min<br />
Temperature: 5 ºC<br />
Detector: UV at 310 nm + PL-LS 15/90º<br />
Sample: Proteins<br />
Overlay of UV and light scattering 90º<br />
for a sample of BSA, illustrating monomer,<br />
dimer, trimer and aggregate peaks<br />
<strong>Column</strong>: ProSEC 300S<br />
PL1147-6501<br />
7.5 x 300 mm, 5 µm<br />
Mobile Phase: 0.1 M KH 2PO 4 containing 0.3 M NaCl, pH 8.0<br />
Flow Rate: 1.0 mL/min<br />
Temperature: 5 ºC<br />
Detector: UV at 310 nm + PL-LS 15/90º<br />
Sample: Proteins<br />
Tips & Tools<br />
Response (mV)<br />
Response (mV)<br />
UV<br />
LS 90º<br />
UV<br />
LS 90º<br />
size exclusion Chromatography (seC)<br />
Retention time (min)<br />
Retention time (min)<br />
Further information can be found in the following publications:<br />
ProSEC 300S Protein Characterization <strong>Column</strong>s (5990- 4 en)<br />
Analysis of Globulins using <strong>Agilent</strong> ProSEC 300S <strong>Column</strong>s (5990- 5 en)<br />
Static Light Scattering Analysis of Globular Proteins with <strong>Agilent</strong> ProSEC 300S <strong>Column</strong>s (5990- 939en)<br />
www.agilent.com/chem/library<br />
UV_Overlay<br />
UV_Overlay_BSA<br />
UHPLC/HPLC<br />
9
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