BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
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Prep to Process PL-SAX and PL-SCX<br />
Purification of a large oligonucleotide<br />
<strong>Column</strong>: PL-SAX 1000Å, 8 µm<br />
Mobile Phase: A: 93% 0.1 M TEAA, pH 7:7% ACN<br />
B: 93% 0.1 M TEAA, 3.24 M ammonium<br />
acetate, pH 7:7% ACN<br />
Gradient: 0-100% B in 20 min<br />
Flow Rate: 1.5 mL/min<br />
Temperature: 60 °C<br />
Detector: UV, 290 nm<br />
Preparative fractionation of a culture<br />
filtrate containing amyloglucosidases<br />
on <strong>Agilent</strong> PL-SAX 4000Å<br />
<strong>Column</strong>: PL-SAX<br />
PL1551-1803<br />
4.6 x 50 mm, 8 µm<br />
Mobile Phase: A: 0.01 M Tris HCI, pH 8<br />
B: A + 0.5 M NaCl, pH 8<br />
Flow Rate: 4.0 mL/min<br />
Gradient: Linear 0-100% B in 2 min<br />
Detector: UV, 280 nm<br />
128 www.agilent.com/chem/biohplc<br />
<strong>Column</strong> Specifications<br />
PL-SAX PL-SCX<br />
Matrix Fully polymeric Fully polymeric<br />
Pore Sizes 1000Å, 4000Å 1000Å, 4000Å<br />
Particle Sizes 10 µm, 30 µm 10 µm, 30 µm<br />
Bead Form Rigid spherical Rigid spherical<br />
Functionality Quaternary amine Sulfonic acid<br />
Pressure Stability 3000 psi 3000 psi<br />
Temperature Stability 80 °C 80 °C<br />
pH Range 1-14 1-14<br />
Eluent Compatibility All anion-exchange buffers All cation-exchange buffers<br />
Packed Bed Density 0.39 g/mL 0.39 g/mL<br />
80<br />
%B<br />
0<br />
0<br />
min<br />
Preparative fraction<br />
91 mer<br />
35<br />
VLC0054<br />
0 min<br />
30
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