BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
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size exclusion Chromatography (seC)<br />
Comparison Between <strong>Agilent</strong> Bio SEC-3 and <strong>Agilent</strong> Bio SEC-5<br />
Analysis of monoclonal antibody<br />
<strong>Column</strong>: Bio SEC-3, 300Å<br />
5190-2511<br />
7.8 x 300 mm, 3 µm<br />
<strong>Column</strong>: Bio SEC-5, 300Å<br />
5190-2526<br />
7.8 x 300 mm, 5 µm<br />
Mobile Phase: 150 mM Sodium Phosphate, pH 7<br />
Flow Rate: 1 mL/min<br />
Detector: UV @ 220 nm<br />
Sample: Humanized monoclonal antibody<br />
The 3 µm column gives higher definition of<br />
fragmentation pattern.<br />
4 www.agilent.com/chem/biohplc<br />
mAU<br />
120<br />
100<br />
80<br />
60<br />
40<br />
20<br />
0<br />
Tips & Tools<br />
0 2 4 6<br />
1<br />
2<br />
3<br />
1. Dimer<br />
2. Monomer<br />
3. Monomer fragment<br />
3 µm<br />
5 µm<br />
8 10 12 14 16 18 min<br />
agilent carries a full line of instrument parts and supplies for<br />
all agilent LC and Ms instruments. For more information go to<br />
www.agilent.com/chem/supplies<br />
Mono_Antibody
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