BioHPLC Column Selection Guide Cover - Agilent Technologies

Purification
Purification Column Selection
Application Technique Notes Agilent Columns
Proteomics Reversed-Phase A specialist high recovery column for proteomics applications. It is designed for µg
scale purifications with maximum recovery.
mRP-C18
All Biomolecules Reversed-Phase High efficiency 300Å silica-based particles. ZORBAX PrepHT 300SB
Synthetic Peptides Reversed-Phase Polymeric material designed for the purification of synthetic peptides. It is a high
efficiency single-column solution for the full range of synthetic peptides, acidic, basic,
hydrophobic and hydrophilic, and covers the size range of peptides produced by both
solution and solid phase synthesis.
VariTide RPC
All Biomolecules Reversed-Phase The premium polymeric reversed-phase family with a range of pore sizes and particle
sizes to enable high efficiency laboratory scale purification using small particle prep
column, and scale-up to high yield production purification with larger particles at the
process scale. Use PLRP-S when purification will be scaled up to produce APIs and will
need regulatory documentation.
PLRP-S
All Biomolecules Ion-Exchange
� 3 µm and 5 µm for high efficiency
� 8 µm, 10 µm, 10-15 µm, 15-20 µm, 30 µm and 50 µm particles for larger scale
and low pressure purification
A fully porous strong anion-exchanger PL-SAX
120 www.agilent.com/chem/biohplc
� 5 µm particle size for high efficiency separations
� 8 µm, 10 µm and 30 µm particles for larger scale medium and low pressure
purification
A fully porous strong cation-exchanger PL-SCX
� 5 µm particle size for high efficiency separations
� 8 µm, 10 µm and 30 µm particles for larger scale medium and low pressure purification
Tips & Tools
Further information can be found in the following publication:
Biomolecule Purification (5990-8335EN)
www.agilent.com/chem/library