BioHPLC Column Selection Guide Cover - Agilent Technologies

More documents

Recommendations

Info

size exclusion Chromatography (seC) 2 www.agilent.com/chem/biohplc ZORBAX GF-250 (USP L33) and GF-450 (USP L35) Gel Filtration Columns Hardware Description Size (mm) Particle Size (µm) Part No. GF-250, 150Å 9.4 x 250 4 884973-901 GF-250, 150Å 4.6 x 250 4 884973-701 GF-450, 300Å 9.4 x 250 6 884973-902 Guard Columns (hardware required) PrepHT Columns GF-250 Diol, Guard Cartridge, 2/pk 9.4 x 15 6 820675-111 GF-250 Diol, Guard Cartridge, 4/pk 4.6 x 12.5 6 820950-911 GF-450 Diol, Guard Cartridge, 2/pk 9.4 x 15 6 820675-111 GF-250 Diol, Guard Cartridge, 4/pk 4.6 x 12.5 6 820950-911 Prep Guard Hardware Kit 840140-901 Guard Hardware Kit 820999-901 PrepHT GF-250, 150Å 21.2 x 250 6 877974-901 PrepHT GF-450, 300Å 21.2 x 250 6 877974-910 PrepHT endfittings, 2/pk 820400-901 PrepHT GF-250, Guard Cartridge, 2/pk 17 x 7.5 5 820212-911 PrepHT GF-450, Guard Cartridge, 2/pk 17 x 7.5 5 820212-911 Guard Cartridge Hardware 820444-901
Agilent PL aquagel-OH SEC Columns for Biomolecule Analysis size exclusion Chromatography (seC) Biomolecule SEC with durability and versatility Aqueous size exclusion chromatography (SEC) is widely used for the determination of molecular weight distributions of naturally occurring water soluble polymers, such as macromolecular biomolecules. This includes natural products and derivatives which may be used as excipients and active ingredients in pharma applications. The PL aquagel-OH series provides a chemically and physically stable matrix for reliable aqueous SEC separations. The columns are packed with macroporous copolymer beads with an extremely hydrophilic polyhydroxyl functionality. The “neutral” surface and the capability to operate across a wide range of eluent conditions provide for high performance analyses of compounds with neutral, ionic and hydrophobic moieties, alone or in combination. PL aquagel-OH is available for analytical and preparative applications. The excellent stability of the PL aquagel-OH packing material allows the eluent to be tailored to suit the polymer, while retaining the high column efficiency. For ionic interactions, the eluent can be modified by the addition of salt and/or the adjustment of pH. For water soluble polymers with a hydrophobic character, only the addition of a weak organic solvent (methanol) is required to inhibit hydrophobic interactions. PL aquagel-OH analytical columns Available with mixed and individual pore sizes, and 5, 8 and 15 µm particle sizes, to cover a very wide range of molecular weights. PL aquagel-OH preparative columns For rapid and convenient scale-up from analytical separations. The columns are packed with the same robust macroporous particles as the analytical column range. UHPLC/HPLC 3
Page 1 and 2:
Agilent BioHPLC Column Selection Gu
Page 3 and 4:
BIOCOLUMN SELECTION GUIDELINES From
Page 5 and 6:
Column Selection Flow Chart Biocolu
Page 7 and 8:
Protein Column Selection Guide Appl
Page 9 and 10:
Separation of charge variants of hu
Page 11 and 12:
Increased resolution for peptide ma
Page 13 and 14:
Preparative scale purification of L
Page 15 and 16:
DNA and RNA Oligonucleotide Column
Page 17 and 18:
Broad Distribution Biomolecules Car
Page 19 and 20:
ReveRseD-PHase HPLC Confidently per
Page 21 and 22:
Sterically Protected 300StableBond
Page 23 and 24:
Improved reproducibility of monoclo
Page 25 and 26:
Short-chain ZORBAX 300SB-C3 is stab
Page 27 and 28:
ZORBAX 300Å StableBond Hardware De
Page 29 and 30:
LC/MS analysis of angiotensin on Ex
Page 31 and 32:
Tips & Tools ZORBAX 300Å Extend-C1
Page 33 and 34: Poroshell 300 columns separate prot
Page 35 and 36: Monoclonal IgG1 chains: Separation
Page 37 and 38: Poroshell 120 • 120Å pore size f
Page 39 and 40: 0 µm 0 µm 0 µm A B C Scanning el
Page 41 and 42: Exploiting chemical stability - TFA
Page 43 and 44: Temperature as a tool to enhance ma
Page 45 and 46: ZORBaX amino acid analysis (aaa) Co
Page 47 and 48: High resolution of 24 amino acids u
Page 49 and 50: Ion-Exchange Column Selection Appli
Page 51 and 52: Consistent ion-exchange MAb separat
Page 53 and 54: Column Specifications Tips & Tools
Page 55 and 56: Weak cation-exchange chromatography
Page 57 and 58: PL-SAX Strong Anion-Exchange Column
Page 59 and 60: Analysis of representative whey pro
Page 61 and 62: Standard protein separation Column:
Page 63 and 64: Agilent Bio-Monolith HPLC Column Se
Page 65 and 66: Baseline expansion of a separation
Page 67 and 68: size exclusion Chromatography (seC)
Page 69 and 70: Column Specifications Agilent Bio S
Page 71 and 72: Comparison of Agilent Bio SEC-3 and
Page 73 and 74: Column Length size exclusion Chroma
Page 75 and 76: Column Specifications Agilent Bio S
Page 77 and 78: Calibration curves - Bio SEC-5 Colu
Page 79 and 80: ProSEC 300S • Mechanically robust
Page 81 and 82: Overlay of UV and light scattering
Page 83: Retention volume versus log (MW) fo
Page 87 and 88: Heparin Column: 2 x PL aquagel-OH 3
Page 89 and 90: Polyethylene Glycol/Oxide standards
Page 91 and 92: aFFiniTY CHROMaTOGRaPHY Agilent Bio
Page 93 and 94: Rapid human polyclonal IgG quantifi
Page 95 and 96: Multiple Affinity Removal System af
Page 97 and 98: Illustration of high abundance prot
Page 99 and 100: mRP-C18 High-Recovery Protein Colum
Page 101 and 102: MeTHOD DeveLOPMenT ZORBAX Column Me
Page 103 and 104: Reversed-Phase LC/MS Methods Method
Page 105 and 106: Method Development Ionic Strength:
Page 107 and 108: Method Development After the initia
Page 109 and 110: Tips & Tools Agilent offers a varie
Page 111 and 112: Human serum: Low abundance protein
Page 113 and 114: 2-D LC/MS Analyses Using ZORBAX Cap
Page 115 and 116: ZORBAX Bio-SCX Series II Capillary
Page 117 and 118: ZORBAX HPLC Capillary Columns (glas
Page 119 and 120: Microbore HPLC for sensitive peptid
Page 121 and 122: BioPharmaceutical Lifecycle Reverse
Page 123 and 124: ZORBAX 300Å StableBond ZORBAX Prep
Page 125 and 126: PLRP-S Prep to Process Application
Page 127 and 128: Prep to Process PLRP-S Size (mm) Pa
Page 129 and 130: PL-SAX AND PL-SCX FOR PREP TO PROCE
Page 131 and 132: Prep to Process PL-SAX and PL-SCX D
Page 133 and 134: Crude peptide screen Column: VariTi
Page 135 and 136:
CARTRIDGE COLUMN SYSTEMS Cartridge
Page 137 and 138:
USP DESIGNATIONS - BIOHPLC COLUMNS
Page 139 and 140:
BioHPLC Columns Literature Title Ag
Page 141 and 142:
BioHPLC Columns Literature Title An
Page 143 and 144:
BioHPLC Columns Literature Title Co
Page 145 and 146:
BIOPHARM DEFINITIONS A active start
Page 147 and 148:
dimer A polymer made up of two iden
Page 149 and 150:
K-L ligase An enzyme that causes mo
Page 151 and 152:
S secondary structure In proteins,
Page 153 and 154:
Poroshell 300 Citations Matilainen,
Page 155 and 156:
Agilent 1260 Infinity Bio-inert Qua
show all

BioHPLC Column Selection Guide Cover - Agilent Technologies

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?