BioHPLC Column Selection Guide Cover - Agilent Technologies

PL-SAX AND PL-SCX
FOR PREP TO PROCESS
Prep to Process PL-SAX and PL-SCX
• Ion-exchange purifications over a wider pH range extend applications
• HPLC flow rates and rapid equilibration reduce purification cycle times
• Large pore size for improved mass transfer delivers high speed, high resolution purifications
These rigid, strong ion-exchange materials are extremely hydrophilic and are designed for purification
of biomolecules. The PL-SAX and PL-SCX materials are totally polymeric and are chemically and
thermally stable over a full range of HPLC conditions. The strong ion-exchange functionalities,
covalently linked to a chemically stable polymer, facilitate ion-exchange purifications over a wider
pH range. This stability can be exploited for column sanitation and clean-up. Thermal stability
also enables the use of denaturing conditions and stabilizing/solubilizing agents for the purification
of target compounds, as encountered in the purification of synthetic oligonucleotides with
self-complementary sequences.
Both the 1000Å and 4000Å wide-pore materials are mechanically stable and robust and can be
operated over a wide range of linear velocities, with fast loading of dilute solutions and wash cycles.
HPLC flow rates and rapid equilibration reduces purification cycle times.
Packing in dynamic axial compression (DAC) column hardware is straightforward and high
efficiency columns are achieved with excellent reproducibility and lifetimes. The 1000Å pore size
is for high-capacity purifications and the 4000Å gigaporous particles with improved mass transfer
are intended for large biomolecules and high-speed, high-resolution purifications.
PURIFICATION
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