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BioHPLC Column Selection Guide Cover - Agilent Technologies

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PL-SAX AND PL-SCX<br />

FOR PREP TO PROCESS<br />

Prep to Process PL-SAX and PL-SCX<br />

• Ion-exchange purifications over a wider pH range extend applications<br />

• HPLC flow rates and rapid equilibration reduce purification cycle times<br />

• Large pore size for improved mass transfer delivers high speed, high resolution purifications<br />

These rigid, strong ion-exchange materials are extremely hydrophilic and are designed for purification<br />

of biomolecules. The PL-SAX and PL-SCX materials are totally polymeric and are chemically and<br />

thermally stable over a full range of HPLC conditions. The strong ion-exchange functionalities,<br />

covalently linked to a chemically stable polymer, facilitate ion-exchange purifications over a wider<br />

pH range. This stability can be exploited for column sanitation and clean-up. Thermal stability<br />

also enables the use of denaturing conditions and stabilizing/solubilizing agents for the purification<br />

of target compounds, as encountered in the purification of synthetic oligonucleotides with<br />

self-complementary sequences.<br />

Both the 1000Å and 4000Å wide-pore materials are mechanically stable and robust and can be<br />

operated over a wide range of linear velocities, with fast loading of dilute solutions and wash cycles.<br />

HPLC flow rates and rapid equilibration reduces purification cycle times.<br />

Packing in dynamic axial compression (DAC) column hardware is straightforward and high<br />

efficiency columns are achieved with excellent reproducibility and lifetimes. The 1000Å pore size<br />

is for high-capacity purifications and the 4000Å gigaporous particles with improved mass transfer<br />

are intended for large biomolecules and high-speed, high-resolution purifications.<br />

PURIFICATION<br />

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