BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
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Method Development<br />
High Sensitivity Capillary <strong>Column</strong> Methods<br />
TFA is generally not used for LC/MS separations of proteins<br />
and peptides. The first step is normally to replace TFA with 0.1<br />
to 1% formic acid. Acetic acid, up to 1% can also be used as<br />
an alternative mobile phase modifier. At low pH, the best<br />
separation may still be obtained with TFA in the mobile phase.<br />
In some cases, the TFA can be displaced post-column with an<br />
alternative acid, such as propionic acid.<br />
0 www.agilent.com/chem/biohplc<br />
Mobile Phase Considerations<br />
Low pH Mid and High pH<br />
LC/MS can also be done at high pH with 10-20 mM NH 4OH<br />
as a mobile phase additive.
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