BioHPLC Column Selection Guide Cover - Agilent Technologies

MicroBore (1.0 mm id) Columns
Separation of a tryptic digest
on ZORBAX MicroBore 300SB-C18
Column: ZORBAX 300SB-C18
863630-902
1.0 x 150 mm, 3.5 µm
Mobile Phase: Gradient: 2-60% B in 60 min
A: 0.1% TFA
B: 0.075% TFA/80% ACN
Flow Rate: 50 µL/min
Temperature: 50 °C
Detector: 215 nm
Sample: 2 µL
Tryptic Digest of rhGH
This example of a tryptic digest separated on a
MicroBore column demonstrates the high sensitivity
and resolution possible with 1.0 mm id columns.
116 www.agilent.com/chem/biohplc
MICROBORE (1.0 MM id) COLUMNS
• High sensitivity for small sample sizes
• Compatible with LC/MS interfaces
• Wide variety of bonded phases
• Silica and polymeric particles
Agilent MicroBore (1.0 mm id) columns are a good choice when sample sizes are limited. They can
improve detection limits 5 times over 2.1 mm id columns when the same sample mass is used. This
increase in sensitivity can be critical. MicroBore columns use low flow rates (typically ~ 50 µL/min).
Therefore, these columns are ideal for use with detectors requiring low flow rates such as some mass
spectrometers and with capillary LC systems.
Optimum performance is achieved when MicroBore columns are used with UHPLC/HPLC
Microbore systems. A wide variety of bonded phases is available for use up to 400 bar including
StableBond, 300SB-C18, 300SB-C8, and Poroshell columns. Polymeric reversed-phase, PLRP-S,
and ion-exchange PL-SAX and PL-SCX are also available for applications requiring exceptionally
stable wide pore particles. Guard columns are also now available with an adjustable tube stop
depth to provide a perfect zero dead volume connection every time.
0 5
10 15 20 25 30 35 40 45 50 55
Time (min)
LCMB001