BioHPLC Column Selection Guide Cover - Agilent Technologies

Capillary and Nano Columns
ZORBAX Nano columns
for high sensitivity protein digest
analysis by LC/MS
Column: ZORBAX 300SB-C18
5065-9911
0.075 x 150 mm, 3.5 µm
Mobile Phase: A: Water + 0.1% Formic acid,
B: ACN + 0.1% Formic acid
Flow Rate: 600 nL/min
Gradient: 2% B to 52% B in 25 min
Detector: Positive Ion Nano Electrospray MS
Sample: 100 fm (1 µL) Digest of 8 Proteins
A ZORBAX Nano HPLC column, 0.075 mm id,
is used for high sensitivity LC/MS analysis of
a protein digest sample.
High sensitivity with capillary columns
Column: ZORBAX SB-C18
5064-8255
0.3 x 150 mm, 5 µm
Column: ZORBAX SB-C18
5064-8256
0.5 x 150 mm, 5 µm
Column: ZORBAX SB-C18
863600-902
1.0 x 150 mm, 3.5 µm
Column: ZORBAX SB-C18
883975-902
4.6 x 150 mm, 5 µm
Sample: 200 ng Biphenyl
108 www.agilent.com/chem/biohplc
Intens.
1.5
1.0
0.5
mAU
1400
1200
1000
800
600
400
300
200
0
2.5 5.0 7.5 10.0 12.5
Time (min)
15.0 17.5 20.0 22.5
0.3 mm
0.5 mm
1.0 mm
0 2.5 5.0 7.5 10.0
Time (min)
12.5 15.0 17.5
Sample-limited applications require capillary column dimensions to minimize on-column sample dilution and to enhance sensitivity. The 0.3 mm
capillary in this example provides 100 times more sensitivity than the standard 4.6 mm column. Agilent Nanobore (0.1 mm to 0.075 mm id)
columns can provide up to 2,000 times more sensitivity for your most limited sample applications.
4.6 mm
LCCN002
LCSB008