BioHPLC Column Selection Guide Cover - Agilent Technologies

size exclusion Chromatography (seC)
Size Exclusion Chromatography (SEC)
Application Agilent Columns Notes
Peptides, proteins Agilent Bio SEC-3 Higher resolution and faster separations from 3 µm particles, with 100Å,
150Å, and 300Å pore sizes.
Large biomolecules and samples
with multiple molecular weight
components
www.agilent.com/chem/biohplc
As a leading manufacturer of SEC columns and instruments for over 30 years, Agilent is continually
developing new SEC products that will provide even higher resolution and quicker separations.
This section highlights Agilent’s broad family of SEC columns for protein biopolymer analysis:
• Bio SEC-3 and Bio SEC-5 columns are available in a variety of pore sizes, and are well suited
for protein analysis – especially when determining the presence of dimers and aggregates in
therapeutic biologicals. Note that 3 µm Bio SEC-3 columns provide higher resolution than our
industry-standard 5 µm Bio SEC-5 columns.
• ProSec 300S columns work well with globular proteins under high salt conditions.
• ZORBAX GF-250 and GF-450 columns are best for preparative SEC of proteins, because
of their larger column size and higher flow rates.
• PL aquagel-OH columns can be used to analyze biopolymers of broad molecular weights,
such as PEGs, oligo- and polysaccharides, starches, and gums.
Agilent Bio SEC-5 More pore size options (100Å, 150Å, 300Å, 500Å, 1000Å, and 2000Å)
to cover a wider range of analytes.
Globular proteins, antibodies ProSEC 300S Single column option for protein analysis in high salt conditions.
Proteins, globular proteins ZORBAX GF-250/450 Higher flow rate capabilities and larger column size for SEC semi-prep
and prep.
Low MW polymers and oligomers, 2 or 3 PL aquagel-OH The PL aquagel-OH analytical series has a pH range of 2-10,
oligosaccharides, PEGs,
lignosulfonates
� PL aquagel-OH 8 µm
� PL aquagel-OH 20 5 µm
� PL aquagel-OH MIXED-M 8 µm
compatibility with organic solvent (up to 50% methanol), mechanical
stability up to 140 bar (2030 psi), and low column operating pressures.
Polydisperse biopolymers, 2 or 3 PL aquagel-OH
polysaccharides, cellulose
derivatives
� PL aquagel-OH MIXED-H 8 µm
� PL aquagel-OH 60/50/40 8 µm
Very high MW polymers,
hyaluronic acids, starches, gums
PL aquagel-OH 60/50/40 15 µm in series