BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
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Reversed-Phase HPLC<br />
Protein elution pattern<br />
on ZORBAX Poroshell 300SB-C8<br />
<strong>Column</strong>: Poroshell 300SB-C8<br />
660750-906<br />
2.1 x 75 mm, 5 µm<br />
Mobile Phase: A: 0.1% TFA in H20 B: 0.1% TFA in ACN<br />
Flow Rate: 1.0 mL/min<br />
Gradient: B: 20 to 70% in 3 min<br />
Detector: UV (214 nm)<br />
Poroshell 300<br />
Hardware Description Size (mm)<br />
34 www.agilent.com/chem/biohplc<br />
<strong>Column</strong> Temperature 40 ºC<br />
500<br />
453<br />
406<br />
359<br />
312<br />
265<br />
218<br />
234<br />
5<br />
6<br />
7<br />
8<br />
1. Glycoprotein X, MW ~ 22 kDa<br />
2. Protein I, MW ~ 4 kDa<br />
3. Glucagon, MW ~ 3.5 kDa<br />
4. Biosynthetic human insulin, MW ~ 6 kDa<br />
5. Protein J, MW ~ 3 kDa<br />
6. Protein K, MW ~ 6 kDa<br />
7. Glycoprotein Y, MW ~ 45 kDa<br />
171<br />
8. Glycoprotein Z, MW ~ 30 kDa<br />
124<br />
77<br />
1<br />
30<br />
0 21 42 63 84 105<br />
Time (s)<br />
126 147 168 189 210<br />
<strong>Column</strong> Temperature 60 ºC<br />
490<br />
444<br />
398<br />
2 3<br />
4<br />
5<br />
6<br />
7<br />
352<br />
306<br />
260<br />
214<br />
168<br />
8<br />
122<br />
76<br />
30<br />
1<br />
0 21 42 63 84 105<br />
Time(s)<br />
126 147 168 189 210<br />
<strong>Column</strong> Temperature 75 ºC<br />
480<br />
435<br />
390<br />
345<br />
3 2<br />
5<br />
4<br />
6<br />
7<br />
8<br />
300<br />
255<br />
210<br />
165<br />
120<br />
75<br />
30<br />
1<br />
0 21 42 63 84 105<br />
Time (s)<br />
126 147 168 189 210<br />
Response (mV)<br />
Response (mV)<br />
Response (mV)<br />
Particle<br />
Size (µm)<br />
Poroshell<br />
300SB-C18<br />
Poroshell<br />
300SB-C8<br />
Prot_Elution<br />
Poroshell<br />
300SB-C3<br />
Poroshell<br />
300Extend-C18<br />
Narrow Bore 2.1 x 75 5 660750-902 660750-906 660750-909 670750-902<br />
MicroBore 1.0 x 75 5 661750-902 661750-906 661750-909 671750-902<br />
Capillary 0.5 x 75 5 5065-4468<br />
Guard Cartridge, 4/pk 2.1 x 12.5 5 821075-920 821075-918 821075-924<br />
Guard Hardware Kit 820999-901 820999-901 820999-901<br />
MicroBore Guard, 3/pk 1.0 x 17 5 5185-5968 5185-5968 5185-5968 5185-5968
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