BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
You also want an ePaper? Increase the reach of your titles
YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.
ReveRseD-PHase HPLC<br />
Confidently perform high-resolution separations<br />
Reversed-Phase HPLC<br />
Reversed-phase UHPLC/HPLC separates solutes based on differences in hydrophobicity, with the<br />
least hydrophobic peak eluting first. This high-resolution technique is capable of separating peptides,<br />
proteins and oligonucleotides that differ by only one amino acid or nucleotide residue.<br />
Because HPLC uses organic solvents (such as acetonitrile, methanol, ethanol and propanol) it is also<br />
a denaturing technique that disrupts a biomolecule’s three-dimensional structure. This allows you to<br />
obtain information about a molecule’s primary structure and sequence, as well as variations in the<br />
sequence to be identified.<br />
<strong>Agilent</strong> offers the industry’s broadest range of wide-pore reversed-phase columns, all backed by<br />
technical support experts and application chemists around the globe. This section features the<br />
following column innovations:<br />
ZORBAX 300Å pore silica columns – an industry first for reversed-phase protein and biomolecule<br />
separations – are available in 6 phases, along with a broad array of sizes. For fast UHPLC<br />
separations, we also offer a 1.8 µm particle size option that withstands pressures up to 1200 bar,<br />
and can be used with high-pressure instruments, such as <strong>Agilent</strong>’s 1290 Infinity LC.<br />
<strong>Agilent</strong> Poroshell columns feature the industry’s first solid core/porous shell particle. Our wide-pore<br />
Poroshell 300 columns are ideal for fast chromatography, and are available in a variety of phases.<br />
<strong>Agilent</strong> PLRP-S columns contain polymer particles, and can be used to separate peptides and<br />
proteins of various sizes and DNA/macromolecular complexes. These columns are unique in that they<br />
are 100% organic modified, can withstand temperatures as high as 200 ºC, and can be used under<br />
conditions from pH 1 to pH 14.<br />
Choose from a range of column sizes, particle sizes (3-8 µm for analytical separations) and pore sizes<br />
(100Å to 4000Å). Preparative columns (10-50 µm) are also available, either prepacked in columns or<br />
as bulk material.<br />
UHPLC/HPLC
Change language