BioHPLC Column Selection Guide Cover - Agilent Technologies

PL-SAX Strong Anion-Exchange Columns
ion-exchange Chromatography
• Small particles deliver excellent chromatographic performance
• Wide range of particle sizes and 2 pore sizes for flexible analysis to scale-up purification
• Exceptional stability for long column lifetime
PL-SAX -N(CH 3) 3 + is ideal for the anion-exchange HPLC separations of proteins, peptides and
deprotected synthetic oligonucleotides under denaturing conditions. The strong anion-exchange
functionality, covalently linked to a chemically stable fully porous polymer, extends the operating pH
range. In addition, the anion-exchange capacity is independent of pH. For synthetic oligonucleotides,
separations using denaturing conditions of temperature, organic solvent, and high pH are all possible.
PL-SAX delivers improved chromatography for self-complementary or G-rich sequences that may
associate to form aggregates or hairpin structures. The 5 µm material provides high efficiency
separations of n and n-1 sequences. A wide range of particle sizes and column geometries permits
analysis scale-up to purification. The strong anion-exchange functionality provides a material with
exceptional chemical and thermal stability, even with sodium hydroxide eluents, leading to long
column lifetime.
Column Specifications
Bonded Phase ID Particle Size Pore Size pH Stability
Operating
Temperature
Limit
Strong Anion-Exchange 2.1, 4.6, 5, 8, 10 1000Å 1-14 80 ºC
7.5, 25, 50
and 100
and 30 and 4000Å
UHPLC/HPLC
55