BioHPLC Column Selection Guide Cover - Agilent Technologies

ZORBaX amino acid analysis (aaa)
Columns and supplies
Reversed-Phase HPLC
ZORBAX Eclipse Amino Acid Analysis (AAA) Columns
• High resolution and rapid analysis of 24 amino acids
• Tested for amino acid analysis
• Uses well-known OPA and FMOC precolumn derivatization chemistry
• Easily automated using a detailed online, derivatization protocol available
for use with Agilent 1100/1200 autosampler
The Agilent ZORBAX Eclipse AAA high efficiency column rapidly separates amino acids following an
updated and improved protocol. Total analysis from injection-to-injection can be achieved in as little
as 14 min (9 min analysis time) on shorter, 75 mm length columns and 24 min (18 min analysis time)
on the 150 mm column length. Exceptional sensitivity (5-50 pmol with DAD, FLD) and reliability are
achieved using both OPA and FMOC derivatization chemistries in one fully automated procedure
using the Agilent 1100/1200 HPLC instrument.
ZORBAX Eclipse Amino Acid Analysis (AAA) Columns
Hardware Description Size (mm) Particle Size (µm) Part No.
Tips & Tools
Analytical routine sensitivity 4.6 x 150 5 993400-902
Analytical routine sensitivity,
high-resolution using FLD
Analytical routine sensitivity,
high-throughput
Solvent Saver high sensitivity,
high-resolution
Further information can be found in the following publication:
High-Speed Amino Acid Analysis (AAA) on 1.8 µm
Reversed-Phase (RP) Columns (59 9- 29 en)
www.agilent.com/chem/library
4.6 x 150 3.5 963400-902
4.6 x 75 3.5 966400-902
3.0 x 150 3.5 961400-302
Guard Catridges, 4/pk 4.6 x 12.5 5 820950-931
Guard Hardware Kit 820888-901
UHPLC/HPLC
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