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BioHPLC Column Selection Guide Cover - Agilent Technologies

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Weak cation-exchange chromatography for P128 therapeutic protein sample<br />

on the <strong>Agilent</strong> 1260 Bio-inert Quaternary LC system using different cation-exchange columns<br />

ion-exchange Chromatography<br />

mAU<br />

400<br />

350<br />

300<br />

250<br />

200<br />

150<br />

100<br />

50<br />

0<br />

<strong>Agilent</strong> Bio MAb 5, 4.6 mm × 250 mm<br />

22 23 24 25 26 27 28 29 30 31 min<br />

24.786<br />

26.359<br />

26.618<br />

mAU<br />

350<br />

300<br />

250<br />

200<br />

150<br />

100<br />

50<br />

0<br />

<strong>Agilent</strong> Bio MAb 10, 4.6 mm × 250 mm<br />

22 23 24 25 26 27 28 29 30 31 min<br />

mAU<br />

140<br />

120<br />

100<br />

80<br />

60<br />

40<br />

20<br />

23.976<br />

25.210<br />

25.860<br />

26.867<br />

27.564<br />

Brand B WCX-10, 4.0 mm × 250 mm<br />

22 23 24 25 26 27 28 29 30 31<br />

min<br />

UHPLC/HPLC<br />

53

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